EVALUATION OF IN VITRO THROMBOLYTIC AND ANTIPROTEINASE ACTIVITIES OF WEDELIA TRILOBATA (LINN.)

Abstract

ABSTRACT

Objective: To determine the phytoconsistutents and in vitro thrombolytic and antiproteinase activities of aqueous extracts of leaf, stem and root of Wedelia trilobata.

Methods: In vitro thrombolytic activity of aqueous extracts of different parts of W. trilobata against thrombolysis. In vitro antiproteinase activity of aqueous extracts of different parts of W. trilobata.

Results: Phytochemical screening of aqueous extracts of leaf, stem and flower of W. trilobata showed various bioactive constituents such as carbohydrates, protein, phenols, tannins, saponins, alkaloids, terpenoids and quinines in all three parts of aqueous extracts. The maximum dissolution of blood clot (thrombolysis or fibrinolysis) was observed in leaf extracts, when compared to stem and extracts of flower W. trilobata. The highest degree of proteinase activity was showed in stem of W. trilobata.

Conclusions: Since the aqueous extracts of different part of W. trilobata possess pharmacological active phytocompounds may be responsible for thrombolytic and antiproteinase activities. In further, to isolate the phytocompounds from this plant may be substantiate an effective drug in pharmacologic aspect as thrombolytic and anti inflammatory therapy.

Keywords: Wedelia trilobata, leaf, stem, flower, aqueous extracts.