FLAVONOIDS FROM SUGAR BEET LEAVES AS HEPATOPROTECTIVE AGENT

Authors

  • Souad E. El-gengaihi Medicinal& Aromatic Plants Dept
  • Manal A Hamed
  • Doha H. Aboubaker
  • Abdel-tawab H. Mossa

Abstract

Objective: This work was designed to investigate the activity of Beta vulgaris (B. vulgaris) extract against hepatotoxicity induced by (carbon tetrachloride) CC14 in male rats.

Methods: Hepatoprotective study was performed on rats, divided into different groups; control healthy rats, the group received B. vulgaris extract, intoxicated rats by CC14, CCl4 group treated with alcoholic leaves extract, and CCl4 intoxicated rats treated with silymarin. The evaluation was done through measuring liver function indices and oxidative stress markers.

Results: The activities of Alanine Transferase (ALT), Aspartate Transferase (AST), Alkaline Phosphatase (ALP), and gamma-glutamyl transferase (GGT) increased by 187.07, 52.37, 50.58, and 94.59% respectively in CCl4 group from control. Supplementation of beet extract decreased this elevation to 10.83, 26.43, 17.07 and 37.21% for the previous parameters respectively. The values obtained of the enzymes activity return nearly to that of control values, also a histopathological investigation of liver confirmed the results obtained.

Conclusion: Beet showed a remarkable anti-hepatotoxic activity against CC14 induced hepatic damage

Keywords: B. vulgaris, Hepatoprotective, Flavonoids, Liver function, Antioxidant enzyme, Histopathology

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References

Refat A, Abdul Ghaffar M. The economic impact of sugar beet cultivation in new buds. Aust J Basic Appl Sci 2010;4:1641-9.

Prejapali N, Purohit S, Sharma A. A handbook of medicinal plant. 1st ed. Publishing House; 2003.

Duke J. Handbook of medicinal herbs. Second Edition. Boca Raton, FL: CRC Press; 2002.

Agrawal M, Srivastava UK, Saxena KK, Kuma A. Hepatoprotective activity of Beta vulgaris against CCl4 included hepatic injury in rats. Fitoterapia 2006;77:91-3.

Murakami T, Matsuda H, Inadzuki M, Hirano K, Yoshikowa N. Medicinal food stuff, XVL sugarbeet: absolute stero structures of beta vulgar sides. Chem Pharm Bull 1999;47:1717-24.

Ranju P, Girhepunje K, Gevariya H, Thirumoorthy N. Hepatoprotective activity of Beta vulgaris against CCl4 induced acute hepatoxicity in rats. Arch Appl Sci Res 2010;2:14-8.

KimI, Chin Y, Lim S, Kim YC, Kim J. Norisoprenoids and hepatoprotective flavone glycosides from the aerial parts of Beta vulgaris var. cicla. Arch Pharm Res 2004;27:600-3.

Slinkard K, Singleton VL. Total phenol analysis automation and comparison with manual methods. Am J Enol Viticult 1977;28:49-55.

Leonardis A, Macciola V, Domenco N. A first pilot study to produce a food antioxidant from sunflower seed shells. Eur J Lipid Sci Technol 2005;107:220-7.

Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidant properties of xanthin on the autooxidation of soya bean oil incyclodextrin emulsion. J Agric Food Chem 1992;40:945-8.

Arun M, Asha VV. Preliminary studies on anti-hepatetoxic effect of Physalis peruviana linn against CCl4 induced acute liver injury in rats. J Ethnopharmacol 2007;111:110-4.

Yuvara J, Subramonim A. Hepatoprotective property of Thespesiapulnea against carbon tetrachloride induced liver damage in rats. J Basic Clin Physiol Pharmacol 2009;20:169-77.

Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978;52:302-10.

Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazinene thiosulphate and molecular oxygen. Biochem Biophys Res Commun 1972;42:849-54.

Moshage H, Kok B, Huizenga JR, Jansan PL. Nitrite and nitrate determination in plasma, A criteical evaluation. Clin Chem 1995;41:892-6.

Gella FJ, Olivella T, Grnz Pastor M, Arenas J, Moreno R, Doban R, et al. A simple procedure for routine determination of Aspartate aminotranseferase and alanine transeferase with pyridaxal. Clin Chim Dec 1985;31:241-7.

Rosalki SB. Automated and semiautomated analysis of non alkaline phosphatases isozymes. J Vet Intern Med 1993;22:633-8.

Szasz G. A kinetic photometric method for serum gamma-glutamyltranspiptidase. Clin Chem 1969;15:124-36.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976;72:248-54.

Benchraft J, Stevens A, Turner D. Theory and practice of histopathological techniques. 4th Ed. Churchil Livengstone: New York; 1996.

Eid N, Ito Y, Maemura K, Otsuki Y. Mitophagy in steatotic hepatocytes of ethanol-treated wild-type and parkin knockout mice. Am J Physiol 2015;309:213-4.

Banskota A, Tezuka Y, Adnyana K, Xiong Q, Hase K, Tran K, et al. Hepatoprotective effect of Commbretum quadrangulare and its constituents. Biol Pharm Bull 2000;23:456-60.

Hayes ML. Guidelines for acute oral toxicity testing’s in animals: principles and methods of toxicology. 2nd Ed. Raven Press: Ltd N. Y; 1989. p. 248.

Anderson N, Borlak J. Mechanism of toxic liver injury chapter 9 pharmaceutical and Medicinal chemistry. Do1 10 1002/978D4705 John Willey and Sons copy right; 2007.

Sekaran S, Kandaswany S, Gunasekaran K. Protective role of quercetin on poly chlorinated biphenyls induced oxidative stress and appoptosis in liver of adult male rats. J Biochem Mol Toxicol 2012;26:522-32.

Chirumbolo S. Role of quercetinin vascular physiology. Can J Physiol Pharmacol 2012;90:1652-7.

Lu J, Wu Dm, Zheng YI. Trace amount of copper exacerbate beta amyloid–induced neurotoxicity in the cholesterol-fed mice through TNF-medicated inflammatory pathway. Brain Behav Immun 2009;23:193-203.

Boots AW, Haenen GR, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharm 2008;585:325-37.

Nabavi SF, Nabavi SM, Mirzaei M, Moghaddam AH. Protective effect of quercetin against sodium fluoride induced oxidative stress in rat's heart. Food Funct 2012;3:437-41.

Bhatt K, Flora S. Oral co-administration of ï¡-lipoic acid, quercetin and captopril prevents gallium arsenide toxicity in rats. Environ Toxicol Pharmacol 2009;28:140-6.

Ghosh A, Mandal AK, Sarkar S, Panda S, Das N. Nanoencapsulation of quercetin enhances its dietary efficacy in combating arsenic-induced oxidative damage in liver and brain of rats. Life Sci 2009;84:75-80.

Graf BA, Ameho C, Dolnikowski CG, Mibury PE, Chen CY, Blumberg JB. Rat gastrointestinal tissue metabolize quercetin. J Nutr 2006;136:39-44.

Van der Woude H, Boersma MG, Vervoort J, Rietjens IM. Identification of 14 quercetin phase II mono-and mixed conjugates and their formation by rat and human phase II in vitro model systems. Chem Res Toxicol 2004;17:1520-30.

Manach C, Morand C, Demigne C, Texier O, Régérat F, Rémésy C. Bioavailability of rutin and quercetin in rats. FEBS Letter 1997;409:12-6.

Published

01-04-2016

How to Cite

El-gengaihi, S. E., M. A. Hamed, D. H. Aboubaker, and A.- tawab H. Mossa. “FLAVONOIDS FROM SUGAR BEET LEAVES AS HEPATOPROTECTIVE AGENT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 4, Apr. 2016, pp. 281-6, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/10121.

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