STUDIES ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT ACTIVITY AND ANTI-BACTERIAL ACTIVITY OF SALACIA OBLONGA STEM EXTRACT
Abstract
Objective: The present study was performed to investigate the phytochemical screening, antioxidant activity and antibacterial activity from the stem extract of Salacia oblonga (S. oblonga).
Methods: The stem extracts were evaluated for antioxidant activities by DPPH (1, 1–Diphenyl-2-picryl-hydrazyl) radical scavenging assay. Different concentrations of aqueous stem extract were tested using the agar disc diffusion technique for the activity against Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli.
Results: The phytochemical analysis revealed the presence of active ingredients such as steroids, saponins, phenols, flavonoids, terpenoids, alkaloids and quinones in the stem extract of S. oblonga. Among the extracts prepared from different solvents (ethanol, aqueous, acetone, petroleum ether and chloroform) the maximum antioxidant activity was found in the aqueous stem extract (84.3±0.0 %) of S. oblonga in Hubli accession followed by others accession like Udipi (72.9±0.25 %) and Jogimat (68.0±0.15%.) The antibacterial activity of aqueous stem extract of S. oblonga shown more active against Bacillus subtilis. It was found to be inactive against Escherichia coli.
Conclusion: It was concluded that the phytochemical screening of aqueous stem extract of S. oblonga in Hubli accession shown strong positive for the availability of natural chemical constituents and also an excellent antioxidant and antibacterial activity.
Keywords: Salacia oblonga, Phytochemicals, Antioxidant, Antibacterial activity, DPPH, Disc diffusion.
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