ANTIMICROBIAL ACTIVITY OF NATURAL CHLOROPHYLLIN FROM ENDANGERED MEDICINAL PLANT MIMOSA PUDICA L.
Keywords:
Medicinal plants, Chlorophyllin, Mimosa pudica, Pathogenic bacteria, Pathogenic fungiAbstract
Objective: Chlorophyllin (CHL) belongs to a group of compounds, porphyrins that contain a chelated metal ion in the center of the molecule. The objective of this present study was to extract the Chlorophyllin from Mimosa pudica and to study its antimicrobial activity.
Methods: The antimicrobial activity of chlorophyllin from the leaf extract of Mimosa pudica L., was determined in vitro, using well diffusion method against human pathogenic bacteria and fungi, viz., two Gram-negative bacteria: Pseudomonas aeruginosa and Escherichia coli, and two Gram-positive bacteria: Staphylococcus aureus and Klebsiella pneomoniae, and one fungal pathogen, Candida albicans.
Results: Chlorophyllin from the leaf extract of Mimosa pudica recorded potential antimicrobial activity against the tested microorganism with the range of 9 mm-18 mm at 25-100 μg ml-1.
Conclusion: Natural Chlorophyllin from Mimosa pudica has significant activity against the five pathogenic test microorganisms.
Keywords: Medicinal plants, Chlorophyllin, Mimosa pudica, Pathogenic bacteria, Pathogenic fungi
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References
Sala AV. Indian medicinal plants: a compendium of 500 species. Chennai: Orient Longman Limited; 1998. p. 36.
Yadava RN, Agrawal PK. A new flavonoid glycoside: 5,7,4ʹ-trihydroxy-6,3ʹ,5ʹ-trimethoxy-flavone7-O-alpha-Larabinopyranosyl(1→6)-O-beta-D-glucopyranoside from the roots of Mimosa rubicaulis. J Asian Nat Prod Res 1998;1:15-9.
Kirtikar KR, Basu BD. Indian medicinal plants. Dehradun: International Book Distributors: 1935. p. 915-7.
Hafsa Ahmad, Sakshi Sehgal, Anurag Mishra, Rajiv Gupta. Mimosa pudica L. (Laajvanti): an overview. Pharmacogn Rev 2012;6:115-24.
Genest S, Kerr C, Shah A, Rahman MM, Saif-E-Naser GM, Nigam P, et al. Comparative bioactivity of two Mimosa species. Lat Am Caribb Bull Med Aromat Plants 2008;7:38-43.
Egner PA, Munoz, Kensler TW. Chemoprevention with chlorophyllin in individuals exposed to dietary aflatoxin. Mutation Res 2003;524:209-16.
Rajalakshmi K, Banu N. Antioxidant capacity of chlorophyllin from Mimosa pudica by the formation of a phospho- molybdenum complex. Int J Front Sci Technol 2014;2:7-14.
Schertz FM. The extraction and separation of Chlorophyll (α+β) carotene and xanthophyll in fresh green leaves preliminary to their quantitative determination. Plant Physiol 1928;3:211–6.
Holder IA, Boyce ST. Agar well diffusion assay testing of bacterial susceptibility to various antimicrobials in concentrations non-toxic for human cells in culture. Burns 1994;20:426-9.
Tona L, Kambu K, Ngimbi N, Cimanga K, Vlietinck AJ. Antiamoebic and phytochemical Spina-Christi sterm bark in rats. Acta Trop 1998;6:1-5.
Samy RP, Ignacimuthu S. Antibacterial activity of some folklore medicinal plants used by tribals in the Western Ghats in India. J Ethnopharmacol 2000;69:63-71.
Palombo EA, Semple SJ. Antibacterial activity of traditional medicinal plants. J Ethnopharmacol 2001;77:151-7.
Kumaraswamy Y, Cox PJ, Jaspars M, Nahar L, Sarker S. Screening seeds of Scottish plants for antibacterial activity. J Ethnopharmacol 2002;83:73-7.
Stepanovic S, Antic N, Dakic I, Svabic-vlahovic M. In vitro antimicrobial activity of propilis and antimicrobial drugs. Microbiol Res 2003;158:353-7.
Ojalla T, Remes S, Hans P. Antimicrobial activity of some coumarin-containing herbal plants growing in Finland. J Ethnopharmacol 1999;68:267-74.
Ahmad I, Beg AZ. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. J Ethnopharmacol 2001;74:113–23.
John A, Alberti KG, Zimmet PZ, Parrotta. Healing plants of peninsular India, CABI publishing USA, 557(2001). Diabetic Med 1998;15:539-53.
Lubna Azmi, Manish Kumar Singh, Ali Kamal Akhtar. Pharmacological and biological overview on Mimosa pudica Linn. Int J Pharm Life Sci 2011;2:1226-34.
Pawaskar SM, Kale KU. Antibacterial activity of successive extracts of Mimosa pudica. Indian Drugs 2006;43:476-80.
Mukesh Chandra Sharma, Smita Sharma. Phytochemical and pharmacological screening of combined Mimosa pudica Linn and Tridax procumbens for in vitro antimicrobial activity. Int J Microbiol Res 2010;1:171-4.