ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF OLIVE TREE EXTRACT

Authors

  • Wafa Laaboudi Biotechnology Laboratory, Faculty of Science Dhar El Mahraz, University Sidi Mohamed Ben Abdellah - Fez – Morocco
  • Jamal Ghanam
  • Hala Aissam
  • Mohammed Merzouki
  • Mohamed Benlemlih

Keywords:

Olive leaves, Olive fruit, Polyphenols, Anti-inflammatory, Analgesic, In vivo

Abstract

Objective: The aim of this study was the in vivo evaluation of analgesic and anti-inflammatory effect of an olive tree extract with high polyphenols content.

Methods: This olive tree extract was obtained from Moroccan olive fruits and leaves using an eco-extraction free of chemical solvents and toxic additives. Anti-inflammatory activity was evaluated using carrageenan and histamine-induced paw edema methods. Analgesic activity of the olive tree extract was estimated against a hot plate, acetic acid induced writhing and formalin tests.

Results: The extracts showed significant anti-inflammatory and analgesic activities with a dose-dependent manner. Anti-inflammatory activity of olive tree extract at 250 and 500 mg/kg doses was more important compared to the used standard drugs (p<0.05), in both carrageenan and histamine-induced paw edema tests. In analgesic assays, results showed that 500 mg/kg dose of olive tree extract has a significant analgesic effect through both peripheral and central mechanisms.

Conclusion: Our findings suggest that olive tree extract is safe and has potential anti-inflammatory and analgesic activities, which promote this use as a food supplement against pain and inflammation related to inflammatory diseases.

Keywords: Olive leaves, Olive fruit, Polyphenols, Anti-inflammatory, and Analgesic, In vivo

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References

Ferrucci L, Corsi A, Lauretani F, Bandinelli S, Bartali B, Taub DD, et al. The origins of the age-related proinflammatory state. Blood 2005;105:2294-9.

Balkwill F, Mantovani A. Inflammation, and cancer: back to Virchow? Lancet 2001;357:539-45.

Williams CS, Mann M, DuBois RN. The role of cyclooxygenases in inflammation, cancer and development. Oncogene 1999;18:7908.

Moreno JJ. Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophages RAW 264.7. Free Radical Biol Med 2003;35:1073-81.

Petroni A, Blasevich M, Salami M, Servili M, Montedoro GF, Galli C. A phenolic antioxidant extracted from olive oil inhibits platelet aggregation and arachidonic acid metabolism in vitro. World Rev Nutr Diet 1994;75:169-72.

Fito M, Cladellas M, De la Torre R, Marti J, Munoz D, Schroder H, et al. Anti-inflammatory effect of virgin olive oil in stable coronary disease patients: a randomized, crossover, controlled trial. Eur J Clin Nutr 2008;62:570-4.

Bogani P, Galli C, Villa M, Visioli F. Postprandial anti-inflammatory and antioxidant effects of extra virgin olive oil. Atherosclerosis 2007;190:181-6.

Rahman I, Biswas SK, Kirkham PA. Regulation of inflammation and redox signaling by dietary polyphenols. Biochem Pharmacol 2006;72:1439-52.

Killeen M, Linder M, Pontoniere P, Crea R. NF-κβ signaling and chronic inflammatory diseases: exploring the potential of natural products to drive new therapeutic opportunities. Drug Discovery Today 2014;19:373-8.

Yamamoto Y, Gaynor RB. Therapeutic potential of inhibition of the NF-kappaB pathway in the treatment of inflammation and cancer. J Clin Invest 2001;107:135-42.

Visioli F, Galli C. Biological properties of olive oil phytochemicals. Crit Rev Food Sci Nutr 2002;42:209-21.

Keys A. Mediterranean diet and public health: Personal reflections. Am J Clin Nutr 1995;61:1321-3.

Stark AH, Madar Z. Olive oil as a functional food: epidemiology and nutritional approaches. Nutr Rev 2002;60:170-6.

Ghanam J, Laaboudi W, Benlemlih M. Effects of rich polyphenols olive tree extract on inflammation and pain in patients with rheumatoid arthritis: an 8-weeks randomized, double-blind, placebo-controlled clinical trial. Int J Biol Pharm Res 2015;2:51-61.

Sergent T, Piront N, Meurice J, Toussaint O, Schneider YJ. Anti-inflammatory effects of dietary phenolic compounds in an in vitro model of the inflamed human intestinal epithelium. Chem Biol Interact 2010;188:659-67.

Beauchamp GK, Keast RSJ, Morel D, Lin J, Pika J, Han Q, et al. Phytochemistry: ibuprofen-like activity in extra-virgin olive oil. Nature 2005;437:45-6.

Esposito E, Mazzon E, Paterniti I, Dal Toso R, Pressi G, Caminiti R, et al. A contributes to the anti-inflammatory activity of verbascoside in a model of inflammatory bowel disease in mice. PPAR Res 2010. Doi:10.1155/2010/917312. [Article in Press]

Speranza L, Franceschelli S, Pesce M, Reale M, Menghini L, Vinciguerra I, et al. Antiinflammatory effects in THP-1 cells treated with verbascoside. Phytother Res 2010;24:1398-404.

Pastore S, Potapovich A, Kostyuk V, Mariani V, Lulli D, De Luca C, et al. Plant polyphenols effectively protect HaCaT cells from ultraviolet C-triggered necrosis and suppress inflammatory chemokine expression. Ann NY Acad Sci 2009;1171:305-13.

Zhang X, Cao J, Zhong L. Hydroxytyrosol inhibits proinflammatory cytokines, iNOS, and COX-2 expression in human monocytic cells. Naunyn Schmiedebergs Arch Pharmacol 2009;379:581-6.

Brunelleschi S, Bardelli C, Amoruso A, Gunella G, Ieri F, Romani A, et al. Minor polar compounds extra-virgin olive oil extract (MPC-OOE) inhibits NF-kB translocation in human monocyte/macrophages. Pharmacol Res 2007;56:542-9.

Muscoli C, Lauro F, Dagostino C, D'Agostino C, Ilari S, Giancotti LA, et al. Olea Europea-derived phenolic products attenuate antinociceptive morphine tolerance: an innovative strategic approach to treat cancer pain. J Biol Regul Homeostatic Agents 2014;28:105-16.

Gong D, Geng C, Jiang L, Cao J, Yoshimura H, Zhong L. Effects of hydroxytyrosol–20 on carrageenan–induced acute inflammation and hyperalgesia in rats. Phytother Res 2009;23:646-50.

Laaboudi W, Ghanam J, Merzouki M, Benlemlih M. Eco-extraction of phenolic compounds from Moroccan olive fruits and leaves and their potential use as antimicrobial agents. Eur J Sci Res 2015;132:255-65.

Creton S, Dewhurst IC, Earl LK, Gehen SC, Guest RL, Hotchkiss JA, et al. Acute toxicity testing of chemicals-Opportunities to avoid redundant testing and use alternative approaches. Crit Rev Toxicol 2010;40:50-83.

Bamgbose SOA, Noamesi BK. Studies on cryptolepine. Inhibition of carrageenan-induced edema by cryptolepine. Planta Med 1981;42:392-6.

Farshid A, Tamaddonfard E, Morvaridi A. Effects of histidine and dexamethasone on the local inflammation induced by histamine in rats. Vet Res Forum 2011;2:31-6.

Sutar NG, Pal SC. Evaluation of analgesic activity of leaf extracts of pergularia daemia [forsk] in experimental animals. Int J Pharm Pharm Sci 2014;6:137-9.

Omisore NOA, Adewunmi CO, Iwalewa EO, Ngadjui BT, Watchueng J, Abegaz BM, et al. Antiniociceptive and anti-inflammatory effects of Dorstenia barteri (Moraceae) leaf and twig extracts in mice. J Ethnopharmacol 2004;95:7-12.

Dahiya RS, Kaur P, Kashyap P, Katoch N, Gupta S. Pharmacological evaluation of hydroalcohol and chloroform extracts of nyctanthes arbour-tristis l. for antioxidant, anti-inflammatory and analgesic activity. Int J Pharm Pharm Sci 2014;6:460-5.

Koster R, Anderson M, De Beer E. Acetic acid for analgesic screening. Fed Proc 1959;18:412.

Mahgoub AA. Grapefruit juice potentiates the anti-inflammatory effects of diclofenac on the carrageenan-induced Rat's Paw oedema. Pharmacol Res 2002;45:1-4.

Hunskaar S, Hole K. The formalin test in mice: dissociation between inflammatory and non-inflammatory pain. Pain 1987;30:103-14.

Raman R, Parthiban S, Karthikeyan S, Muthuraman MS, Sivasubramanian A. Antimicrobial and anti-inflammatory studies on sargassum wightii extracts. Int J Pharm Pharm Sci 2014;6:611-4.

Ribeiro IA, Rocha J, Sepodes B, Mota-Filipe H, Ribeiro MH. Effect of naringin enzymatic hydrolysis towards naringenin on the anti-inflammatory activity of both compounds. J Mol Catal B: Enzym 2008;52:8-13.

Vineger R, Truax JF, Selph JL. Quantitative studies of the pathway to acute carrageenan inflammation. Fed Proc 1976;35:2447-56.

Di Rosa M, Giroud JP, Willoughby DA. Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. J Pathol 1971;104:15-29.

Vasudevan M, Gunman KK, Parle M. Antinociceptive and anti-inflammatory effects of Thespesia populnea bark extract. J Ethnopharmacol 2007;109:264-70.

Linardi A, Costa SKP, DeSilva GR, Antunes E. Involvement of kinins, mast cells, and sensory neurons in the plasma exudation and paw edema induced by staphylococcal entrotoxin B in the mouse. Eur J Pharmacol 2002;399:235-42.

Cuman RKN, Bersani-Amadio CA, Fortes ZB. Influence of type 2 diabetes on the inflammatory response in the rat. Inflam Res 2001;50:460-5.

Maiuri MC, De Stefano D, Di Meglio PP, Irace C, Savarese M, Sacchi R. Hydroxytyrosol, a phenolic compound from virgin olive oil, prevents macrophage activation. Naunyn Schmiedebergs Arch Pharmacol 2005;371:457-65.

Cornwell DG, Ma J. Nutritional benefit of olive oil: the biological effects of hydroxytyrosol and its arylating quinone adducts. J Agric Food Chem 2008;56:8774-86.

Smith CJ, Zhang Y, Koboldt CM, Muhammad J, Zweifel BS, Shaffer A. Pharmacological analysis ofcyclooxygenase-1 in inflammation. Proc Natl Acad Sci USA 1998;95:133313-8.

Hiruma-Lima C, Gracioso J, Bighetti E, Germonsen Robineou L, Souza Brito A. The juice of fresh leaves of Boerhaavia diffusa L. (Nyctaginaceae) markedly reduces pain in mice. J Ethnopharmacol 2000;71:267-74.

Zakaria ZA, Ghani ZDFA, Nor RNSRM, Gopalan HK, Sulaiman MR, Jais AMM, et al. Antinociceptive, anti-inflammatory, and antipyretic properties of an aqueous extract of Dicranopteris linearis leaves in experimental animal models. J Nat Med 2008;62:179-87.

Turner RA. Screening methods in pharmacology. Acad Press NY; 1965.

Sofidiya MO, Oloruntola OM, Sofola I, Fageyinbo MS. Antinociceptive activity of Euadenia trifoliolata (Schum. & Thonn.) Oliv. Leaves and roots in mice. Journal of Traditional Complementary Medicine 2015;1-5. Doi:10.1016/j. jtcme. 2015.07.006. [Article in Press]

Garcia R, Ferreira JP, Costa G, Santos T, Branco F, Caramona M. Evaluation of anti-inflammatory and analgesic activities of Cymbopogon citratus in vivo-polyphenols contribution. J Med Plants Res 2015;9:1-13.

Mbagwu H, Anene R, Adeyemi O. Analgesic, antipyretic and anti-inflammatory properties of Mezoneuron benthamianum Baill (Caesalpiniaceae). Niger Q J Hosp Med 2007;17:35-41.

Vidyalakshmi K, Kamalakannan P, Viswanathan S, Ramaswamy S. Antinociceptive effect of certain dihydroxy flavones in mice. Pharmacol Biochem Behav 2010;96:1-6.

Valerio DA, Georgetti SR, Magro DA, Casagrande R, Cunha TM, Vicentini FT, et al. Quercetin reduces inflammatory pain: Inhibition of oxidative stress and cytokine production. J Nat Prod 2009;72:1975-9.

Haloui E, Marzouk B, Marzouk Z, Bouraoui A, Fenina N. Hydroxytyrosol and oleuropein from olive leaves: potent anti-inflammatory and analgesic activities. J Food Agric Environ 2011;9:128-33.

Published

01-07-2016

How to Cite

Laaboudi, W., J. Ghanam, H. Aissam, M. Merzouki, and M. Benlemlih. “ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF OLIVE TREE EXTRACT”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 7, July 2016, pp. 414-9, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/12109.

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