EFFECT OF SELECTED HEAVY METALS (LEAD AND ZINC) ON SEEDLING GROWTH OF SOYBEAN GLYCINE MAX (L.) MERR

Authors

  • Siddhi Gupta Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India 302004
  • Manoj Kr. Meena
  • Soumana Datta

Keywords:

Lead, Zinc, Seed germination, Glycine max (L) Merr

Abstract

Objective: This study was designed to evaluate the effects of different concentrations of both zinc (250, 500, 750, 1000 and 1250 mg/kg) and lead (200, 400, 600, 800, 1000 mg/kg) as zinc sulphate and lead acetate respectively on 7days seedling of soybean (Glycine max (L.) Merr.)

Methods: To investigate morphological growth parameters seedlings were cut at the root-shoot junction and the length of their root and shoot was measured with a metric scale and expressed in centimetre's. The fresh weight of seedling samples was recorded on an analytical balance and expressed in gram per seedling. Later, seedlings were dried in an oven at 80o C for 24 h to get constant dry weight. After 24 h the dry weight was recorded.

Results: The study revealed that elevated dose of lead concentrations reduces the growth parameter as compared to control. Lead concentrations of 1000 mg/kg significantly decreased the percentage of germination and root length. However, at low levels of zinc (250 and 500 mg/kg) showed increased germination percentage and also increase root length shoot length. But at high levels (750–1250 mg/kg) showed a detrimental effect on the growth parameter and germination.

Conclusion: Consequently, higher concentrations of heavy metals had an increased inhibitory effect on seed germination percentage, root length, shoot length, tolerance index, fresh weight and dry weight of soybean seedlings, but the low concentration of zinc can be applied for increasing the growth and yield of soybean plants.

Downloads

Download data is not yet available.

References

Sinha R, Singh SN, Gupta AK. Impact of bimetallic combinations of Cu and Ni on percentage germination and early seedling growth of Vigna mungo L. Cultivars. Plant Arch 2012;12:383-6.

McGrath SP, Zhao FJ, Lombi E. Plant and rhizosphere processes involved in phytoremediation of metal-contaminated soils. Plant Soil 2001;232:207-14.

Bennett F. Nutrient deficiencies and toxicities in crop plants (Edit). The American Phytopathological Society, USA; 1993.

Choi JM, Pak CH, Lee CW. Micronutrient toxicity in French marigold. J Plant Nutr 1996;19:901-16.

Rathore H, Punyasi R, Joshi P, Rathore D, Bhatnagar D. Studies on the reversal of lead induced mitostatic effect in Allium cepa root tip cells with myrobalan (fruit of Terminalia chebula, Retz, Combretaceae). Internet J Alternative Med 2007;4 Suppl 1:6.

Akinci IE, Akinci S, Yilmaz K. Response of tomato (Solanum lycopersicum L.) to lead toxicity: growth, element uptake, chlorophyll and water content. Afr J Agric Res 2010;5:416-23.

Liu X, Zhang S, Shan X, Zhu YG. Toxicity of arsenate and arsenite on germination, seedling growth and amylolytic activity of wheat. Chemosphere 2005;61:293-301.

Akinci IE, Akinci S. Effect of chromium toxicity on germination and early seedling growth in melon (Cucumis melo L.). Afr J Biotechnol 2010;9:4589-94.

Amin H, Arain BA, Amin F, Surhio MA. Analysis of growth response and tolerance index of Glycine max (L.) Merr. under hexavalent chromium stress. Adv Life Sci 2014;1:231-41.

Manivasagaperumal R, Balamurugan S, Thiyagarajan G, Sekar J. Effect of zinc on germination, seedling growth and biochemical content of cluster bean (Cyamopsistetra gonoloba (L.) taub. Curr Bot 2011;2:11-5.

Reichman SM. The response of plant to metal toxicity: a review of focusing on copper, manganese and zinc. Australian Minerals Energy Environ Foundation 2002;7:59.

Morzck EJ, Funicclli NA. Effect of lead and on germination of Spartina alterniflora losiel seeds at various salinities. Environ Exp Bot 1982;22:23-32.

Nakos G. Lead pollution: fate of lead in soil and its effects on Pinus haplenis. Plant Soil 1979;50:159-61.

Islam E, Yang X, Li T, Liu D, Jin X, Meng F. Effect of Pb toxicity on root morphology, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. J Hazard Mater 2007;147:806-16.

Sengar RS, Gautam M, Sengar RS, Sengar RS, Garg SK, Sengar K, et al. Lead stress effects on physio biochemical activities of higher plants. Rev Environ Contam Toxicol 2009;196:1-21.

Muhammad S, Iqbal ZM, Mohammad A. Effect of lead and cadmium on germination and seedling growth of Leucaena leucocephala. J Appl Sci Environ Manage 2008;12:61-6.

Vijayarengan P. Changes in growth, biochemical constituents and antioxidant potentials in cluster bean Cyamopsis tetragonoloba L. Taub under zinc stress. Int J Curr Sci 2013;5:37-49.

Wang C, Tian Y, Wang X, Geng J, Jiang J, Yu H, et al. Lead contaminated soil induced oxidative stress, defense response and its indicative biomarkers in roots of Vicia faba seedlings. Ecotoxicology 2010;19:1130-9.

Kumar G, Singh R, Sushila. Nitrate assimilation and biomass production in Sesamum indicum (L) seedling in lead enriched environment. Water Air Soil Pollut 1992;215:124-215.

Iqbal J, Mushtaq S. Effect of lead on germination, early seedling growth, soluble protein and acid phosphate content in Zea maize. Pak J Sci Ind Res 1987;30:853-6.

Chaoui A, Mazhoudi S, Ghorbal MH, Ferjani EE. Cadmium and zinc induction of lipid peroxidation and effects on antioxidant enzyme activities in bean (Phaseolus vulgaris L.). Plant Sci 1997;127:139-47.

Panda SK. Chromium-mediated oxidative stress and ultrastructural changes in root cells of developing rice seedlings. J Plant Physiol 2007;164:1419-28.

Published

01-08-2016

How to Cite

Gupta, S., M. K. Meena, and S. Datta. “EFFECT OF SELECTED HEAVY METALS (LEAD AND ZINC) ON SEEDLING GROWTH OF SOYBEAN GLYCINE MAX (L.) MERR”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 8, Aug. 2016, pp. 302-5, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/12616.

Issue

Section

Original Article(s)