SYSTEMATIC SCREENING FOR PHYTOCHEMICALS OF VARIOUS SOLVENT EXTRACTS OFTHEVETIA PERUVIANA SCHUM. LEAVES AND FRUIT RIND

Authors

  • Nazneen Rahman Department of Biotechnology and Bioinformatics, Kuvempu University, Jnanasahyadri, Shankarghatta 577451, Shimoga Dist. Karnataka, India.
  • Riaz Mahmood Department of Biotechnology and Bioinformatics, Kuvempu University, Jnanasahyadri, Shankarghatta 577451, Shimoga Dist. Karnataka, India.
  • Haseebur Rahman Department of Biotechnology and Bioinformatics, Kuvempu University, Jnanasahyadri, Shankarghatta 577451, Shimoga Dist. Karnataka, India.
  • Mir Haris Department of Biotechnology and Bioinformatics, Kuvempu University, Jnanasahyadri, Shankarghatta 577451, Shimoga Dist. Karnataka, India.

Keywords:

Thevetia peruviana (S), Yellow oleander, Phytochemical, Alkaloids, Cardiac glycosides

Abstract

Thevetia peruviana (S) (yellow oleander) is a tropical evergreen shrub or small tree that is in the Apocynaceae family as and closely related to Nerium oleander, is mainly known for its cardiac glycosides, which was traditionally used as antipyretic [10], as emetics, diuretics and tonics [12].In addition, its extensive use reported for antitumor therapy [11] and therefore it has important medicinal values.

Objective: The present investigation deals with thorough phytochemical analysis of leaf and fruit rind of Thevetia peruviana (S). This study represents a first report of quantitative and qualitative determination of phytochemicals of T. peruviana (S).

Methods: Plant leaves and fruit rind material extracted by using solvents of increasing polarity and the quantity of crude extracts obtained. The ethanol extract has shown highest percentage of yield in both the samples followed by water in fruit rind, than hexane and chloroform extracts. Furthermore, pilot solubility tests performed for the selection of vehicle solvents.

Results: The qualitative phytochemical studies have clearly demonstrated that the plant Thevetia peruviana (S)is a rich source ofalkaloids,flavonoids, saponins, cardiac glycosides, anthroquinone glycosides, coumarins, phenols, tannins, steroids, oils and fats.Further quantitative determination confirmed that T. peruviana (S) is very rich in alkaloid 52.92 ± 0.06 mg/g (leaf) and 59.28 ± 0.04 mg/g (fruit rind), saponins 7.97 ± 0.36 mg/g (leaf) and 14.27 ± 1.02 mg/g (fruit rind)followed by flavonoid and phenolics.

Conclusion: Among the extracts, the ethanolic extract has found to be rich in phytoconstituents, which gives a very strong reason to select this plant for cytotoxic evaluation and other pharmacological properties.

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References

Rishton GM.. Natural products as a robust source of new drugs, drug leads:past successes and present day issues. Am J Cardiol 2008;101(10A):43D-49D.

Salminen A, Lehtonen M, Suuronen T, Kaarniranta K, Huuskonen J. Terpenoids:natural inhibitors of NF-kB signalling with anti-inflammatory and anticancer potential. J Cell Mol Life Sci 2008;65(19):2979-99.

Sennblad B, Bremer B. Classification of Apocynaceaes. L. according to a new approach combining Linnaean and phylogenetic taxonomy. J Syst Biol 2002;51:389-409.

Alvarado-Cardenas L. La Familia Apocynaceae en el Valle de Tehuacan-Cuicatlan (Puebla-Oaxaca). B.Sc. Thesis, Facultad de Ciencias UNAM. J Mexico 2003.

Siddiqui BS, Begum S, Siddiqui S, Lichter W. Two cytotoxic pentacyclic triterpenoids from Nerium oleander. J Phytochem.1995;39 (1):171-4.

Singh CU, Streeper R. Water soluble formulations of Digitalis Glycosides for treating Cell-proliferation and other diseases. J U S Pat Appl Publ 2005;0026849:A1.

Laudadio C, Devis M. Cardiac Glycosides for treatig muscle pain and spam. U S Pat Appl Publ 2003;0229029:A1.

Oji O, Madubuike FN, Nwalozie MC. Mortality in rats following dietary inclusion of Thevetia neriifolia seeds. J Fitoterapia 1993;64:137-9.

PanigrahiA, Raut SK. Thevetia peruviana in the control of slug and snail pests. J Mem Inst Oswaldo Cruz 1994;89(2):247-50.

Oliver-Bever B. Medicinal Plants in Tropical West Africa:Cambridge University Press Cambridge;1986. p. 366.

Richard W. Relationships between plant Folklore and Antitumor Activity:a historical review. J SIDA Contributions to Botany.2005;21(4):2205-41.

Rasoanaivo P, Petitjean A, Conan JY. Toxic and poisonous plants of Madagascar Ãan ethno pharmacological survey. J Fitoterapia 1993;64(2):114-29.

Jackson, BP., Snowdown DW. Powdered vegetable drugs, Cheer Chil Ltd:London;1968.p.25.

Pratt RJ, Chase CR. Fluorescence of Powdered vegetable drug with particular reference to development of a system of identification. J Am Pharm Assoc 1949;38:324-33.

Kokoshi CJ, Kokoshi RJ. Fluorescence of powdered vegetable drugs under Ultraviolet radiation. J Am Pharm Assoc 1958;47:715-7.

Harborne JB. Phytochemical methods. Chapman and Hall, Ltd;1973.p.49-188.

Obdoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some Homeostatic plants in Edo and Delta States of Nigeria. Glo J Pure App Sci 2001;8:203-8.

Chandler SF, Dodds JH. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. J Plant Cell Rep1993;2:105-10.

Woisky R., Salatino A. Analysis of Propolis:Some parameters and procedures for chemical quality control J Api Res 1998;37:99-105.

Seifter S, Dayton S, Novic B, Muntwyler E. Quantitative detection of glycogen fractions by anthrone method. J Arch Biochem Biophys 1950;25:191.

Hammuel C, Abdullahi MS, Mankilik M, Anyinm BP, Adesina OB., Inekwe UV. et al. The Phytochemical and Antimicrobial Activities of Oil from the Seed of Thevetia peruviana Plant. J Appl Envi Bio Sci 2011;1(2):597-601.

Kumar P, Khatri P, Gopi J, Shukla P, Patel R. Pharmacognostic and Preliminary Physiochemical Investigations of Thevetia peruviana (Pers.) K. Schum Flowers International. J Res Pharm Sci 2012;2(1):100-8.

Ansari SH, Essentials of Pharmacognosy.1stEdn.Birla Publications Pvt. Ltd. New Delhi;2006.

Published

31-08-2014

How to Cite

Rahman, N., R. Mahmood, H. Rahman, and M. Haris. “SYSTEMATIC SCREENING FOR PHYTOCHEMICALS OF VARIOUS SOLVENT EXTRACTS OFTHEVETIA PERUVIANA SCHUM. LEAVES AND FRUIT RIND”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 173-9, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/1318.

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