VALIDATION AND TRANSFERABILITY OF SIMPLE SEQUENCE REPEATS (SSR'S) FROM SOME SPECIES OF ACACIA GENUS TO ACACIA NILOTICA L.

Amita Yadav; Manila Yadav; Sandeep Kumar; Dushyant Sharma; Jaya Parkash Yadav

doi:10.22159/ijpps.2016v8i11.13550

Authors

Amita Yadav Department of Genetics, Maharshi Dayanand University, Rohtak 124001, India
Manila Yadav Department of Genetics, Maharshi Dayanand University, Rohtak 124001, India
Sandeep Kumar Department of Genetics, Maharshi Dayanand University, Rohtak 124001, India
Dushyant Sharma Department of Genetics, Maharshi Dayanand University, Rohtak 124001, India
Jaya Parkash Yadav Department of Genetics, Maharshi Dayanand University, Rohtak 124001, India

DOI:

https://doi.org/10.22159/ijpps.2016v8i11.13550

Keywords:

Acacia nilotica, SSR, Genetic diversity, Transferability, Geographical distance

Abstract

Objective: In present study genetic transferability of SSR's from related Acacia species to Acacia nilotica was evaluated along with its genetic diversity analysis from north Indian region.

Methods: A total of 30 primers selected from 5 different Acacia species were screened for amplification and polymorphism. Dendrogram and 2 D Plot were constructed using NTSys PC version 2.02e. Different diversity parameters like Polymorphism information content (PIC), alleles per primer, number (no.) of amplicons were also calculated for each primer pair.

Results: SSRs from Acacia tortilis, A. senegal and A. koa were highly transferable in A. nilotica. Out of 30, only twenty-two primers showed amplification with an average of 1.36 alleles per locus. Polymorphic information content (PIC) values ranged from 0.5 to 0.96 with an average of 0.81. Jaccard similarity coefficient (J) values ranged from 0.04 to 0.67 showing a high level of diversity. Un-weighted pair group method with arithmetic mean (UPGMA), based cluster analysis, divided all accessions into three main clusters.

Conclusion: Geographical and climatic conditions showed a great impact on genetic diversity. The results indicated high transferability of genomic resources from related species and will facilitate more studies to characterize the relatively less studied Acacia niloticagenome.

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