POTENTIAL ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIBACTERIAL EVALUATION OF EXTRACTS OF LEUCAS ASPERA USING IN VITRO MODELS
DOI:
https://doi.org/10.22159/ijpps.2016v8i11.13711Keywords:
Albumin denaturation, Anti-inflammatory, Antimicrobial, Hemolysis, lipooxygenase, Phytochemicals, Leucas asperaAbstract
Objective: To evaluate the potential antioxidant, anti-inflammatory and antibacterial activities of aqueous and methanolic extracts of leaves of Leucas aspera (Thumbae).
Methods: Phytochemical screening of the leaves of L. aspera was followed by analysis of antioxidant activity by means of DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity. In vitro antiâ€inflammatory activity was evaluated using lipoxygenase inhibition, albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a standard drug for the study of antiâ€inflammatory activity. Linear regression analysis was used to calculate half maximal inhibitory concentration, IC50 value. The zone of inhibition was performed against common pathogens to determine the antimicrobial activity at different concentrations of plant extracts (60%, 70%, 80%).
Results: The phytochemical analysis revealed the presence of carbohydrates, amino acid, alkaloids, tannins, flavonoids, glycosides, xanthoproteins, and phenols. The total phenolic and flavonoid content was found to be 2.25±0.04 mg GAE/g (gallic acid equivalents) and 1.2±0.05 mg QE/g (Quercetin equivalents) of fresh weight tissue respectively. The IC50 values for hydrogen peroxide scavenging activity were found to be 244.6 µg/ml. The extract inhibited the lipoxygenase enzyme activity with an IC50 value of 356.3 µg/ml. Maximum inhibition of heat-induced protein denaturation of 69% was observed at 400 μg/ml, IC50 249.6 μg/ml. Proteinase activity was also significantly inhibited (IC50 = 421.6 μg/ml). Membrane stabilization assay attributed minor protection by the leaf extract with an IC50 of 206.7. It was observed that E. coli were inhibited at all concentrations, followed by Klebsiella and Pseudomonas.
Conclusion: Results indicate that L. aspera possess anti-inflammatory properties due to the strong occurrence of polyphenolic compounds such as alkaloids, flavonoids, tannins and steroids that serve as free radical inhibitors or scavenger. Compounds of the plant L. aspera may hence be used as lead compounds for designing potent anti-inflammatory drug which can be used for treatment of various diseases.
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Chopra RN, Nayar SL, Chopra IC. In: Glossary of Indian medicinal plants. Vol. I. Council of Scientific and Industrial Research, New Delhi; 1956. p. 197.
Balunas MJ, Kinghorn AD. Drug discovery from medicinal plants. Life Sci 2005;78:431-41.
Tambe VD, Tambe VB, Jadhav RS. Evaluation of Leucas aspera whole plant extracts for the diuretic and laxative property. Asian J Pharm Clin Res 2016;9:118-21.
Hedge IC. Labiatae. In: Ali SI, Nasir YJ. editors. Flora of Pakistan. Karachi: University of Karachi Department of Botany; 1990. p. 192.
Santosh KM, Anil KS, Ankit S. Potential medicinal plants for lymphatic filariasis: a review. J Crit Rev 2015;2:1-6.
Girach RD, Aminuddin, Siddioui PA, Khan SA. Traditional plant remedies among the Kondh of district dhenkanal (Orissa). Int J Pharm 1994;32:274–83.
Bhandary MJ, Chandrasekhar KR, Kaveriappa KM. Medical ethnobotany of the siddis of Uttara Kannada district, Karnataka. India J Ethnopharmacol 1995;47:149–58.
Selvanayahgam ZE, Gnanevendhan SG, Balakrishna K, Rao RB. Antisnake venom botanicals from ethnomedicine. J Herbs Spices Med Plants Plants 1994;2:45–100.
Khaleque A, Huq ME, Huq MS, Mansoor MH. Chemical investigations on leucas aspera. I. Isolation of compound a, beta-sitosterol and alpha-sitosterol from the aerial parts. Sci Res 1970;7:125–7.
Kapur SK, Sarin YK. Medico-botanic survey of medicinal and aromatic plants of Katra valley (J. and K. state) India. Indian Drugs 1984;2:4–10.
John D. One hundred useful raw drugs of the kani tribes of Trivandrum forest division, Kerala, India. Int J Crude Drug Res 1984;22:17–39.
Alby AB, Regi RK. Evaluation of the folk claim and identification of the active pharmacological principles in Bauhinia phoenicea leaves. Asian J Pharm Clin Res 2016;9:1-4.
Mukherjee PK, Saha K, Murugesan T, Mandal SC, Pal M, Saha BP. Screening of anti-diarrhoeal profile of some plant extracts of a specific region of west Bengal, India. J Ethnopharmacol 1998;60:85–9.
Saha K, Mukherjee PK, Das J, Mandal SC, Pal M, Saha BP. Hypoglycemic activity of Leucas lavandulaefolia Rees. In streptozotocin-induced diabetic rats. Phytother Res 1998;11:463–6.
Mangathayaru K, Grace FX, Bhavani M, Meignanam E, Karna RS, Kumar PD. Effect of Leucas aspera on hepatotoxicity in rats. Indian J Pharmacol 2005;37:329–30.
Krishnaraju AV, Rao TV, Sundararaju D, Vanisree M, Tsay HS, Subbaraju GV. Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. Int J Appl Sci Eng 2005;3:125–34.
Muthukrishnan J, Puphlantha H, Kasthuribhai K. Biological effects of four plants extracts on Culex quinquefasciatus Say. Larval stages. Insect Sci Its Appl 1997;17:389–94.
Okeke IN, Klugman KP, Bhutta ZA, Duse AG, Jenkins P, O’Brien TF. Antimicrobial resistance in developing countries. Part II: strategies for containment. Lancet Infect Dis 2005;5:568–80.
Abiramasundari P, Priya V, Jeyanthi GP, Gayathri DS. Evaluation of the antibacterial activity of cocculus hirsutus. Hygeia: J Drugs Med 2011;2:26-31.
Sandosskumar R, Karthikeyan M, Mathiyazhagan S, Mohankumar M, Chandrasekar G, Velazhahan R. Inhibition of Aspergillus flavus growth and detoxification of aflatoxin B1 by the medicinal plant zimmu (Allium sativum L. x Allium cepa L.). World J Microbiol Biotechnol 2007;23:1007-14.
Gupta SK, Banerjee AB. Screening of selected West Bengal plants for antifungal activity. Ecol Bot 1972;26:255-9.
Sofowara A. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd. Ibadan: Nigeria; 1993. p. 289-300.
Harborne JB. Phytochemical Methods. Chapman and hall Ltd, London: UK; 1973. p. 49-188, 11.
Ogbuewu IP. Physiological responses of rabbits fed graded levels of neem (Azadirachta indica) leaf meal. Federal University of Technology: Owerri; 2008.
Slinkard K, Singleton VL. Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic 1977;28:49-55.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
Braca A, Sortino C, Politi M. Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol 2002;79:379-81.
Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.
Axelrod BC, Cheesbrough TM, Laasko SL. Lipoxygenase from soybean. Methods Enzymol 1981;71:441-51.
Mizushima Y, Kobayashi M. Interaction of antiâ€inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169â€73.
Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharm Pharmacol Sci 2010;2:146-55.
Oyedepo OO, Femurewa AJ. Antiâ€protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Int J Pharmacogn 1995;33:65â€9.
Azeem AK, Dilip C, Prasanth SS, Junise V, Hanan S. Anti-inflammatory activity of the glandular extracts of Thunnus alalunga. Asia Pac J Med 2010;3:412-20.
Negi JS, Singh P, Rawat B. Chemical constituents and biological importance of swertia: a review. Curr Res Chem 2011;3:1-15.
Shwetha RJ, Tahareen S, Myrene RD. Antioxidant and anti-inflammatory activity of Tinospora cordifolia using in vitro models. J Chem Biol Phys Sci 2006;6:497-512.
Rio DA, Obdululio BG, Casfillo J, Marin FG, Ortuno A. Uses and properties of citrus flavonoids. J Agric Food Chem 1997;45:4505-15.
Okwu DE. Phytochemicals and vitamin content of indigenous spices of southeastern Nigeria. J Sustain Agric Environ 2004;6:30-7.
Shah BA, Qazi GN, Taneja SC. Boswellic acids: a group of medicinally important compounds. Nat Prod Rep 2009;26:72-89.
The United States Department of Agriculture. Center for Nutrition Policy and Promotion. Dietary Guidelines for Americans. National Academy Press: Washington DC: USA; 2010.
Ikyenge BA, Odiniya EO, Okhale SE. Preliminary phytochemical and pharmacognostic investigation of laggerapter odonta (DC) Sch. Bip. Int J Tradit Nat Med 2010;1:1-7.
Amarowicz R. Tannins: the new natural antioxidants? Eur J Lipid Sci Technol 2007;109:549–51.
Veitch NC. Isoflavonoids of the leguminosae. Nat Prod Rep 2007;24:417-64.
Jiang H, Zhan WQ, Liu X, Jiang X. Antioxidant activities of extracts and flavonoid compounds from Oxytropis falcate Bunge. Nat Prod Res 2008;22:1650-6.
Kim HP, Son HK, Chang HW, Kang SS. Anti-inflammatory plant flavonoids and cellular action mechanisms. J Phamacol Sci 2004;96:229-45.
Thabrew MI, Hughes RD, McFarlane IG. Antioxidant activity of Osbeckia aspera. Phytother Res 1998;12:288–90.
Gardner HW. Recent investigations into the lipoxygenase pathway of plants. Biochim Biophys Acta 1991;1084:221–39.
Abad MJ, Bermejo P, Villar A. The activity of flavonoids extracted from Tanacetum microphyllum DC. (Compositae) on soybean lipoxygenase and prostaglandin synthetase. Gen Pharmacol 1995;26:815–9.
Das SN, Chatterjee S. Long-term toxicity study of ARTâ€400. Indian Indg Med 1995;16:117-23.
Shenoy S, Shwetha K, Prabhu K, Maradi R, Bairy KL, Shanbhag T. Evaluation of anti-inflammatory activity of Tephrosia purpurea in rats. Asian Pac J Trop Med 2010;3:193-5.
El-Shabrany OA, El-Gindi OD, Melek FR, Abdel-Khalk SM, Haggig MM. Biological properties of saponin mixtures of Fagonia cretica and Fagonia mollis. Fitoterapia LXVIII 1997;68:219-22.
Saad S, Taher M, Susanti D, Qaralleh H, Awang AF. In vitro antimicrobial activity of mangrove plant Sonneratia alba. Asian Pac J Trop Biomed 2012;2:427–9.
Mohammed AA, Khalil AA, El-Beltagi HE. Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica). Grases Y Acites 2010;61:67–75.
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