IMMUNOMODULATORY ACTIVITY OF NUTRACEUTICAL FORMULATION AND ITS POTENTIATION BY SELF-FORTIFICATION AND COW URINE DISTILLATE FORTIFICATION METHODS

Annapurna Akula; Chandi Vishala; Gummalla Pitchaiah

doi:10.22159/ijpps.2017v9i8.13716

Authors

Annapurna Akula College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India
Chandi Vishala College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India
Gummalla Pitchaiah College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India

DOI:

https://doi.org/10.22159/ijpps.2017v9i8.13716

Keywords:

Nutraceutical formulation, Fortification, Cell mediated immunity, Humoral immunity and Phagocytic activity

Abstract

Objective: This study prepared, evaluated immunomodulatory activity of nutraceutical formulation and studied the effect of self-mortification and cow urine distillate fortification methods on the immunomodulatory potential of nutraceutical formulation.

Methods: Three types of nutraceutical formulations i.e. Nutraceutical formulation (NF), self fortitfied nutraceutical formulation (SFNF) and self fortitfied nutraceutical formulation fortified with cow urine distillate (SFNECUD) were prepared using fine powders of amla, apple, garlic, onion, wheat grass, papaya, turmeric and cow urine distillate by different methods. The immunomodulatory activity of nutraceutical formulations at a dose of 500 mg/kg was assessed by various immune function parameters like cell-mediated immunity (neutrophil adhesion, delayed type hypersensitivity (DTH) response and cyclophosphamide-induced neutropenia), humoral immunity (serum immunoglobulins level and haemoagglutination antibody titer), and phagocytic activity (carbon clearance and polymorphonuclear (PMN) cell activity).

Results: Oral administration of NF, SFNF and SFNFCUD showed significant (p<0.01) increase in adhesion of neutrophils, potentiation of the DTH reaction and attenuation of cyclophosphamide-induced neutropenia. A significant increase in serum immunoglobulin levels and production of circulating antibody titer in response to sheep red blood cells (SRBCs) was also observed. In addition, an increase in the phagocytic index in carbon clearance assay and an increase in the phagocytic activity of PMN cells was observed.

Conclusion: From the above results, it can be concluded that all three types of formulations showed significant immunostimulant activity. SFNF and SFNFCUD showed better immunomodulatory activity than NF suggesting the potentiation of immunomodulatory potential of NF activity by fortification methods.

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