IN VITRO ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF THE ETHANOLIC EXTRACTS OF TERMINALIA BELLERICA ROXB (BAHEDA)

Rasna Gupta; Ram Lakhan Singh; Neeraja Dwivedi

doi:10.22159/ijpps.2016v8i11.14175

Authors

Rasna Gupta Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad-224001, U.P., India
Ram Lakhan Singh Nutraceutical Laboratory, Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad 224001, U.P., India
Neeraja Dwivedi Department of Biotechnology, IFTM University, Moradabad-244102, U.P., India

DOI:

https://doi.org/10.22159/ijpps.2016v8i11.14175

Keywords:

Reactive oxygen species, Antioxidant, GC-MS, Phenolics, Phytochemicals

Abstract

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.

Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.

Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC₅₀ for FRSA (62 Âµg/ml), SARSA (39 Âµg/ml) and HRSA (27 Âµg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC₅₀ for LPO (48 Âµg/ml) whereas TBS showed lowest IC₅₀ for FTC (109 Âµg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.

Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.

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