DETERMINATION OF 10-GINGEROL IN INDIAN GINGER BY VALIDATED HPTLC METHOD OF SAMPLES COLLECTED ACROSS SUBCONTINENT OF INDIA

Kamran Ashraf; Syed Adnan Ali Shah; Mohd Mujeeb

doi:10.22159/ijpps.2016v8i12.14953

Authors

Kamran Ashraf Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia
Syed Adnan Ali Shah Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia
Mohd Mujeeb Bioactive Natural Product Laboratory, Faculty of Pharmacy, Jamia Hamdard University, New Delhi 11062 (India)

DOI:

https://doi.org/10.22159/ijpps.2016v8i12.14953

Keywords:

Ginger, 10-gingerol, HPTLC, Validation

Abstract

Objective: A simple, sensitive, precise, and accurate stability indicating HPTLC (high-performance thin-layer chromatography) method for analysis of 10-gingerol in ginger has been developed and validated as perICH guidelines.

Methods: The separation was achieved on TLC (thin layer chromatography) aluminum plates pre-coated with silica gel 60F₂₅₄ using n-hexane: ethyl acetate 55:45 (%, v/v) as a mobile phase. Densitometric analysis was performed at 569 nm.

Results: This system was found to have a compact spot of 10-gingerol at R_F value of 0.57Â±0.03. For the proposed procedure, linearity (r² = 0.998Â±0.02), limit of detection (18ng/spot), limit of quantification (42 ng/spot), recovery (ranging from 98.35%â€“100.68%), were found to be satisfactory.

Conclusion: Statistical analysis reveals that the content of 10-gingerol in different geographical region varied significantly. The highest and lowest concentration of 10-gingerol in ginger was found to be present in a sample of Patna, Lucknow and Surat respectively which inferred that the variety of ginger found in Patna, Lucknow are much superior to other regions of India.

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