ELICITATION OF THERAPEUTIC POTENTIAL AND OXIDATIVE STRESS ASSESSMENT OF FENUGREEK SPROUTS UNDER UV IRRADIATION
DOI:
https://doi.org/10.22159/ijpps.2017v9i5.15647Keywords:
Trigonella foenum-graecum, Antidiabetic activity, Antioxidant enzymes, Oxidative stress, Phenolic compounds, UV irradiationAbstract
Objective: To evaluate the effect of ultraviolet (UV) radiation on the therapeutical and oxidative stress status of fenugreek seedlings during the post-germination phase.
Methods: The seeds of Trigonella foenum-graecum L. were subjected to UV radiation of different wavelengths UV 254 nm, UV 365 nm and the combination of 254 nm and 365 nm UV during the course of germination up to 96 h. Free radicals scavenging activity, in vitro antidiabetic activity as well as total phenol (TPC) and flavonoid (TFC) contents were evaluated up to four days of germination stages. Analysis of total activities of superoxide dismutase (SOD), peroxidase, catalase and NADPH oxidase (NOX) was conducted using non-denaturing polyacrylamide gel electrophoresis along with histochemical detection of H2O2 and superoxide radical localisation, lipid peroxidation and plasma membrane integrity was also performed.
Results: Overall it was observed that the sprouts exposed to UV 365 nm exhibited a significant enhancement in free radical scavenging as well as antidiabetic activity at the 48h stage of germination. Interestingly highest phenol and flavonol content were also stimulated at the same stage by UV 365 nm. The effect of UV irradiation was evident on the localisation of H2O2 and superoxide radical. The on gel assay revealed that the total activities of the antioxidant enzymes were extensively elevated by UV irradiation.
Conclusion: The results suggest that the UV irradiation technology can be implemented for the enhancement of nutraceutical properties of sprouts also UV exposure helps in the induction of the antioxidant enzyme system which may be beneficial for oxidative stress management in plants during germination phases.
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Marton M, Mandoki Zs, Csapo-Kiss Zs, Csapo J. The role of sprouts in human nutrition. A review. Acta Univ Sapientiae Aliment 2010;3:81-117.
Valko M, Leibfritz D, Moncola J, Cronin MTD, Mazura M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007;39:44-84.
Ashwini AM, Majumdar M. Quantification of phytochemical contents and in vitro antioxidant activity of Exacum bicolor (Roxb.), an endemic medicinal plant. Int J Pharm Pharm Sci 2015;7:225-30.
Irshad M, Chaudhuri PS. Oxidant-antioxidant system: role and significance in human body. Indian J Exp Biol 2002;40:1233-9.
Jha S, Ghosal M, Gupta SK, Ghosh A, Mandal P. In vitro free radical scavenging potential of oligopeptides derived from wheat and mung bean. Int J Pharm Pharm Sci 2016;8:428-32.
Hayden MR, Tyagi SC. Is type 2 diabetes mellitus a vascular disease (atheroscleropathy) with hyperglycemia a late manifestation? The role of NOS, NO and redox stress. Cardiovasc Diebetol 2003;2:2-12.
Jaiswal J, Bhardwaj H, Srivastava S, Gautam H, Sharma S. Anti-diabetic activity of methanolic extract of Calotropis gigantea seeds on stz induced diabetic rats. Int J Pharm Pharm Sci 2014;6:254-7.
Meghwal M, Goswami TK. A Review on the functional properties, nutritional content, medicinal utilization and potential application of fenugreek. J Food Process Technol 2012. Doi:10.4172/2157-7110.1000181.
Frohnmeyer H, Staiger D. Ultraviolet-B radiation-mediated response in plants: balancing damage and protection. Plant Physiol 2003;133:1420-8.
Charles MT, Kalantari S, Corcuff R, Arul J. Post-harvest quality and sensory evaluation of UV-treated tomato fruit. Acta Hort 2005;682:537-42.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-1200.
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 1999;26:1231.
Dinis TCP, Madeira VMC, Almeida LM. Action of phenolic derivates (acetaminophen, salicylate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161–9.
Moein MR, Moein S, Ahmadizadeh S. Radical scavenging and reducing power of Salvia mirzayanii subfractions. Molecules 2008;13:2804-13.
Keles A, Koca I, Genccelep H. Antioxidant properties of wild edible mushrooms. J Food Process Technol 2011;2:130-6.
Dewanto V, Wu X, Adom KK, Liu RH. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J Agric Food Chem 2002;50:3010-4.
Ghosal M, Mandal P. In vitro antidiabetic and antioxidant activity of Calamus erectus Roxb. fruit: a wild plant of Darjeeling Himalaya. Int J Pharm Biol Sci 2013;4:671-84.
Gupta SK, Mandal P. Free radical scavenging activity and histochemical localisation of reactive oxygen species in fenugreek sprouts primed with nitric oxide donors. Am J Phytomed Clin Ther 2014;2:1310-22.
Kaur S, Singh HP, Batish DR, Kohli RK. Artemisia scoparia essential oil inhibited root growth involves reactive oxygen species (ROS)-mediated disruption of oxidative metabolism: in vivo ROS detection and alterations in antioxidant enzymes. Biochem Sys Ecol 2012;44:390-9.
Pereira GJG, Molina SMG, Lea PJ, Azevdo RA. Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea. Plant Soil 2002;239:123-32.
Verma S, Dubey RS. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 2003;164:645-55.
Janda T, Szalai G, Tari I, Paldi E. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta 1999;208:175-80.
Frahry G, Schopfer P. NADH-stimulated, cyanide-resistant superoxide production in maize coleoptiles analyzed with a tetrazolium-based assay. Planta 2001;212:175-83.
Kalidhasan N, Bhagavan NB, Kannan ND. Ultraviolet-B (280-320 nm) enhanced radiation-induced changes in secondary metabolites and photosystem-II of medicinal plant Withania somnifera Dunal. J Med Plant Res 2013;7:3112-20.
Alothman M, Bhat R, Karim A. Effects of radiation processing on phytochemicals and antioxidants in plant produce. Trends Food Sci Technol 2009;20:201-12.
Rozema J, Bjorn LO, Bornman JF, Gaberscik A, Hader DP, Trost T, et al. The role of UV-B radiation in aquatic and terrestrial ecosystems-an experimental and functional analysis of the evolution of UV-absorbing compounds. J Photochem Photobiol B 2002;66:2-12.
Gonzalez-Aguilar GA, Zavaleta-Gatica R, Tiznado-Hernandez ME. Improving the postharvest quality of mango ‘Haden’ by UV-C treatment. Postharvest Biol Technol 2007;45:108-16.
Neto CC, Amoroso JW, Liberty AM. Anticancer activities of cranberry phytochemicals: an update. Mol Nutr Food Res 2008;52:18-27.
Ghosal M, Chhetri PK, Ghosh MK, Mandal P. Changes in antioxidant activity of Cyphomandra betacea (Cav.) Sendtn. fruits during maturation and senescence. Int J Food Prop 2013;16:1552-64.
Shinar E, Rachmilewitz EA. Oxidation denaturation of red blood cells in thalassemia. Semin Hematol 1990;27:70-91.
Gupta SK, Ghosal M, Choudhury D, Mandal P. Dynamic changes in antioxidant activity during floral development of Couroupita guianensis. Br J Pharm Res 2014;4:676-94.
Tarling CA, Woods K, Brastianos HC, Zhang R, Brayer GD, Andersen RJ, et al. The search for novel human pancreatic α-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts. Chem Biochem 2008;9:433-8.
Saravanan S, Parimelazhagan T. In vitro antioxidant, anti-microbial and anti-diabetic properties of polyphenols of Passiflora ligularis Juss. fruit pulp. Food Science and Human Wellness 2014;3:56-64.
Shobana S, Sreerama YN, Malleshi N. Composition and enzyme inhibitory properties of finger millet (Eleusine coracana L.) seed coat phenolics: mode of inhibition of α-glucosidase and pancreatic amylase. Food Chem 2009;115:1268-73.
Mai TT, Nghiem NT, Pham GT, Nguyen VC. Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. J Nutr Sci Vitaminol 2007;53:267-76.
Bowler C, Van M, Inze MD. Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol Plant Mol Biol 1992;43:83-116.
Kramer GF, Norman HA, Krizek DT, Mirechi RM. Oxidation and membrane lipids on cucumber. Phytochemisty 1991;30:2101-18.
Reshmi GR, Rajalakshmi R. Drought and UV stress response in Spilanthes acmella Murr., (tooth-ache plant). J Stress Physiol Biochem 2012;8:110-29.
Hideg E, Jansen MAK, Strid A. UV-B exposure, ROS, and stress: inseparable companions or loosely linked associates? Trends Plant Sci 2013;18:107-15.
Balakrishnan V, Venkatesan K, Ravindran KC, Kulandaivelu G. Protective mechanism in UV-B treated Crotalaria juncea L. seedlings. Plant Protect Sci 2005;41:115-20.
Abdel Nasser LEA. Effects of UV-B radiation on some physiological and biochemical aspects in two cultivars of barley (Hordeum vulgare L.). Egypt J Biol 2001;3:97-105.
Jiang MY, Zhang JH. Involvement of plasma membrane NADPH oxidase in abscisic acid-and water stress-induced antioxidant defense in leaves of maize seedlings. Planta 2002;215:1022-30.
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