IN VITRO CYTOTOXICITY EFFECT ON MCF-7 CELL LINE OF CO-ENCAPSULATED ARTESUNATE AND CURCUMIN LIPOSOME
DOI:
https://doi.org/10.22159/ijpps.2017v9i3.15872Keywords:
In vitro cytotoxicity, Lipophilic interaction, MCF-7Abstract
Objective: Cancer drug delivery has focused using novel carrier system due to their high drug loading and release controlled property and minimum side effect. Artesunate (ART) and curcumin (CUR) are proved natural herbal lead chemotherapeutics used as Chinese and Indian food. But due to its low absorption and the poor bioavailability limits its clinical efficacy. The object of the present work was to investigate the cytotoxic vs apoptotic effect of these herbal lead molecules in the co-encapsulated liposome formulation.
Methods: ART and CUR liposome were prepared by thin film hydration method. Because of their opposite solubility nature, ART loaded in the aqueous phase at pH 7.4 while CUR is present in the central core through lipophilic interaction. In vitro cytotoxicity action of the free and liposomal formulation was performed by MTT assay.
Results: Physiochemical parameters were evaluated and compared to the co-encapsulated formulation of containing both molecules. The mean diameter around 200 nm, low polydispersity index and co-encapsulation efficiency were found to be 90%. Co-encapsulation in nanometer size is beneficial for uptake and photo-stability of a drug. The in vitro cytotoxic effect of the co-encapsulated formulation was shown a better result than individual drug and also gives the clue for apoptosis. IC50 value for ART, CUR and Liposome was found to be 297.61 µg/ml and 60.60 µg/ml respectively. The result explained the co-encapsulation of curcumin with artesunate is show an ameliorative effect for repositioning therapeutic efficacy of the drug.
Conclusion: We observed that human breast cancer MCF-7 cells were relatively resistant to ART and sensitive to CUR. Treatment with ART plus CUR had a synergistic cytotoxic, and apoptotic effect was mediated by up regulation of DR4 and DR5 m RNA expression.
Downloads
References
Irvin W, Muss HB, Mayer DK. Symptom management in metastatic breast cancer. Oncol 2011;16:1203-14.
Aftab N, Vieira A. Antioxidant activities of curcumin and combinations of this curcuminoid with other phytochemicals. Phyto Res 2010;24:500-2.
Malhotra A, Nair P, Dhawan DK. Modulatory effects of curcumin and resveratrol on lung carcinogenesis in mice. J Phytol Res 2010;24:1271-7.
Berger TG, Dieckmann D, Efferth T, Schultz ES, Funk JO, Baur A, et al. Artesunate in the treatment of metastatic uveal melanoma-first experiences. Oncol Rep 2005;14:1599-604.
Hasan M. Liposome encapsulation of curcumin: physico-chemical characterizations and effects on MCF7 cancer cell proliferation. Int J Pharm 2014;461:519-28.
Yen YA, Herenyiova M, Weber G. Quercetin: synergistic action with carboxy amido triazole in human breast carcinoma cells. Life Sci 1995;57:1285-92.
Coradini K, Lima FO, Oliveira CM, Chaves PS, Athayde ML, Carvalho LM, et al. Co-encapsulation of resveratrol and curcumin in lipid-core nanocapsules improves there in vitro antioxidant effects. Eur J Pharm Biopharm 2014;88:178-85.
Sharma S, Saraogi GK, Kumar V. Development of spectrophotometric methods for simultaneous determination of artesunate and curcumin in a liposomal formulation. Int J Appl Pharm 2015;7:18-21.
Wang S. The promise of cancer therapeutics targeting the TNF-related apoptosis-inducing ligand and TRAIL receptor pathway. Oncogene 2008;27:6207-15.
Truneh A, Sharma S, Silverman C, Khandekar S, Reddy MP, Deen KC, et al. Temperature-sensitive differential affinity of TRAIL for its receptors DR5 is the highest affinity receptor. J Biol Chem 2000;275:23319-25.
Ashkenazi A, Holland P, Eckhardt SG. Ligand-based targeting of apoptosis in cancer: the potential of recombinant human apoptosis ligand 2/tumor necrosis factor–related apoptosis-inducing ligand (rhApo2L/TRAIL). J Clin Oncol 2008;26:3621-30.
McDonald ER, Chui PC, Martelli PF, Dicker DT, El-Deiry WS. Death domain mutagenesis of KILLER/DR5 reveals residues critical for apoptotic signaling. J Biol Chem 2001;276:14939-45.
Lacour S, Hammann A, Wotawa A, Corcos L, Solary E, Dimanche-Boitrel MT. Anticancer agents sensitize tumor cells to tumor necrosis factor related apoptosis inducing ligand-mediated caspase-8 activation and apoptosis. Cancer Res 2001;61:1645-51.
Gibson SB, Oyer R, Spalding AC, Anderson SM, Johnson GL. Increased expression of death receptors 4 and 5 synergizes the apoptosis response to combined treatment with etoposide and TRAIL. Mol Cell Biol 2000;20:205-12.
Henson ES, Johnston JB, Gibson SB. The role of TRAIL death receptors in the treatment of haematological malignancies. Leuk Lymphoma 2008;49:27-35.
Liu P, Wang Z, Brown S, Kannappan V, Erebi-Tawari P, Jiang W, et al. Liposome-encapsulated Disulfiram inhibits NFκB pathway and targets breast cancer stem cells in vitro and in vivo. Oncotarget 2014;5:7471-85.
Tian Y, Mao S. Amphiphilic polymeric micelles as the Nanocarrier for peroral delivery of poorly soluble anticancer drugs. Eu ODD 2012;9:687-700.
Yoon YI, Kwon YS, Cho HS, Heo SH, Park KS, Park SG, et al. Ultrasound-mediated gene and drug delivery using a microbubble-liposome particle system. Theranostics 2014; 4:1133-44.
Senthilraja P, Kathiresan K. In vitro cytotoxicity MTT assay in Vero, HepG2 and MCF-7 cell lines study of marine yeast. J Appl Pharm Sci 2016;5:80-4.
Moorthi C, Kumar CS, Kathiresan K. Synergistic anti-cancer activity of curcumin and bio-enhancers combination against various cancer cell lines. Int J Pharm Pharm Sci 2014;6:901-3.
Published
How to Cite
Issue
Section
