STUDY THE RELATIONSHIP BETWEEN ANTIOXIDANT POTENTIAL AND PHENOLIC CONTENTS OF JUNIPERUS EXCELSA FRUIT
Keywords:
Juniperus excelsa fruit, Fractions, Reducing power, DPPH radical scavenging Phenolic contentsAbstract
Objective: Today's attentions are focused on the finding new natural antioxidant compounds because of their fewer side effects than synthetic antioxidants. The aim of this study was to determine the antioxidant potentials of Juniperus excelsa fruit and its fractions by different methods.
Methods: For evaluation antioxidant potentials, DPPH radical scavenging, determination of reducing power and phenolics were used. Gallic acid and quercetin were used as antioxidant standards.
Results: The highest DPPH radical scavenging was observed in n-butanol fraction (IC 50= 135.9±2.5 µg/ml) of Juniperus excelsa fruit. Also, this fraction possesses the highest reducing power (in 61.4±2.6 µg/ml with absorbance 0.5) and phenolic contents (82. 9±1.1 mg/g).
Conclusion: n-butanol fraction of Juniperus excelsa fruit had the highest radical scavenging, reducing power and phenolic compounds. In other words, a relationship between antioxidant potentials and phenolic compounds was found. Anyway, this fraction is a strong source of antioxidant compounds and can be used as a natural antioxidant.
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