DOSE-DEPENDENT AMELIORATION OF EPIGALLOCATECHIN-3-GALLATE AGAINST SODIUM VALPROATE INDUCED AUTISTIC RATS
DOI:
https://doi.org/10.22159/ijpps.2017v9i4.17283Keywords:
Autism, Neurotransmitters, VPA, EGCGAbstract
Objective: Autism is a neurodevelopment related disorder with a range of clinical presentations attending serious behavioral and neurological disorders among young children that now occur at epidemic rates in developing countries, India included. The objective of this research was to study the effect of epigallocatechin gallate (EGCG) on sodium valproate-induced autism rats.
Methods: On the 12th day of gestation wistar rats were administered with a single intraperitoneal injection of sodium valproate (VPA) (600 mg/kg body weight), which induced autism. The rats were treated with EGCG in varying doses 1, 2 and 5 mg/kg body weight via oral administration. The neuroprotectivity effect of the EGCG was followed by assessing the neurotransmitters and neurobiochemical activities such as serotonin, glutamate and nitrite levels in hippocampus and cerebellum region of the brain.
Results: Early prenatal exposure to VPA provokes autistic symptoms. Induction of autism significantly impinged the neurotransmitters and neurochemicals such as serotonin, glutamate and nitrite levels in the brain (hippocampus and cerebellum) increased significantly in the rats exposed to VPA. After treatment with an effective dose of EGCG 2 mg/kg body weight the neurotransmitters and neurochemicals levels were decreased when compared with control and VPA-exposed rats.
Conclusion: EGCG ameliorates and reverses autistic attributes possibly due to its neuroprotective activity which could pave the way for future investigation for the possible therapeutic approach.
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Bauman ML, Kemper TL. Histoanatomic observations of the brain in early infantile autism. Neurology 1985;35:866-874.
Rodier PM, Ingram JL, Tisdale B, Croog VJ. Linking etiologies in humans and animal models: studies of autism. Reprod Toxicol 1997;11:417-422.
Ingram JL, Peckham SM, Tisdale B, Rodier PM. Prenatal exposure of rats to valproic acid reproduces the cerebellar anomalies associated with autism. Neurotoxicol Teratol 2000,22:319-324.
George C. Wagner, Kenneth R. Reuhl, Michelle Cheh, Paulette McRae, Alycia K. Halladay. A new Neurobehavioral model of autism in mice: Pre and postnatal Exposure to sodium valproate. J Autism Dev Disord 2006;36:779-793.
Hossein Fatemi S, Amelia, Merz, George, Realmuto. The roles of reelin, bcl2, and serotonin in cerebellar pathology in autism. J Develop Phys Disab 2003;15:1-22.
Sandhya T, Sowjanya J, Veeresh B. Bacopa monniera (L.) Wettst Ameliorates behavioral alterations and oxidative markers in sodium valproate-induced Autism in Rats. Neurochem Res 2012;37:1121-1131.
Kumaravel P, SubashS, Seethalakshmi KS, Murugan N, YuvarajanR, Subramanian P. Monosodium glutamate modulates the circadian rhythms of biochemical variables and behavioral activity in rats under constant light. Int J Nutr Pharmacol Neurol Dis 2012;2:251-257.
Guo S, Yan J, Yang T, Yang X, Bezard E, Zhao B. Protective effects of green tea polyphenols in the 6-OHDA rat model of Parkinson’s disease through inhibition of ROS-NO pathway. Biol Psychiatry 2007;62:1353-1362.
Elena Lecumberri, Yves Marc Dupertuis, Raymond Miralbell, Claude Pichard. Green tea polyphenol EGCG as an adjuvant in cancer therapy. Clin Nutr2013;32:894-903.
Ji Seon Kim, Jong-Min Kim, Jeong-Ja O, Beom S. Jeon. Inhibition of inducible nitric oxide synthase expression and cell death by EGCG, a green tea catechin, in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson's disease. J of Clin Neuro 2010;17:1165-1168.
Adam Smith, Brian Giunta, Paula C. Bickford, Michael Fountain,
Jun Tan, R. Douglas Shytle. Nanolipidic particles improve the bioavailability and secretase inducing ability of EGCG for the treatment of Alzheimer's disease. Intr J Pharm 2010;389:207-212.
Tourandokht B, Mehrdad R. Chronic epigallocatechin-3 gallate ameliorates learning and memory deficits in diabetic rats via modulation of nitric oxide and oxidative stress. Behav Brain Res 2011;224:305-310.
Maickel RP, Cox RH, Saillant J, Miller FP. A method for the determination of serotonin and norepinephrine in discrete areas of rat brain. Int J Neuropharmacol 1968;7:275-281.
Jacobowitz DM, Richardson JS. A method for the rapid determination of norepinephrine, dopamine and serotonin in the same brain region. Pharmacol Biochem Behav 1978;8:515-519.
Green LCDA, Wagner DA. Analysis of nitrate, nitrite and [15 N] nitrate in biological fluids. Anal Biochem 1982;126:131-138.
Tsikas D. Analysis of nitrite and nitrate in biological fluids by assays based on the Gries reaction: an appraisal of the Gries reaction in the L-arginine/nitric oxide area of research. J Chromatogr B Anal Technol Biomed Life Sci 2007;851:51-70.
Lowe IP, Robins E, Eyermann GJ. The fluorometric measurement of glutamic decarboxylase and its distribution in the brain. J Neurochem 1958;3:8-18.
Berger-Sweeney J, Meadows K, Mills J, Hohmann CF. Gender differences in the effect of neonatal basal forebrain lesions on spatial navigation. Soc Neurosci Abstr 1993;19:1233.
Barbara S. Mesmere. New Autism Research Developments. Newyork: Nova Science Publishers; 2007. p.29-46.
Bauman NL and Kemper TL, Neuroanatomic observations of the brain in autism, in the neurobiology of autism. Baltimore, Md, USA, Johns Hopkins University Press; 1994. p. 119-145.
Schmahmann JD. The cerebellum and cognition. San Diego, Academic Press; 1997. p. 665.
Miyazaki K, Narita N, Narita M. Maternal administration of thalidomide or valproic acid causes abnormal serotonergic neurons in the offspring: implication for pathogenesis of autism. Int J Dev Neurosci 2005;23:287-297.
Charles PD, Ambigapathy G, Geraldine P, Akbarsha MA, Rajan KE. Bacopa monniera leaf extract up-regulates tryptophan hydroxylase (TPH2) and serotonin transporter (SERT) expression: Implications in memory formation. J Ethnopharmacol 2011;134:55-61.
Rebeca Mejias, Abby Adamczyk, Victor Anggono, Tejasvi Niranjan, Gareth M. Thomas, Kamal Sharma, et al. Gain-of-function glutamate receptor interacting protein 1 variants alter GluA2 recycling and surface distribution in patients with autism. Proc Natl Acad Sci 2011;108:4920-4925.
Roberta Bristot Silvestrin, Victorio Bambini-Junior, Fabiana Galland, Larissa Daniele Bobermim, Andre Quincozes Santos, Renata Torres Abib, et al. Animal model of autism induced by prenatal exposure to valproate: altered glutamate metabolism in the hippocampus. Brain Res 2013;1495:52-60.
Archana Panche, Sheela Chandra, Diwan AD, Sanjay Harke. Alzheimer’s and current therapeutics: a review. Asian J Pharm Clin Res 2015;8:14-19.
Suganuma M, Okabe S, Oniyama M, Tada Y, Ito H. and Fujiki H. Wide distribution of [3H]-(2)-epigallocatechin gallate, a cancer preventive tea polyphenol, in mouse tissue. Carcinogenesis 1998;19:1771-1776.
Seong-Ryong Leea, Seong-Il Suhb, Sang Pyo Kimc. Protective effects of the green tea polyphenol (2)-epigallocatechin gallate against hippocampal neuronal damage after
transient global ischemia in gerbils. Neurosci Lett 2000;287: 191-194.
Bolanos JP, Almeida A, Stewart V, Peuchen S, Land JM, Clark JB, et al. Nitric oxide-mediated mitochondrial damage in the brain: mechanisms and implications for neurodegenerative diseases. J Neurochem 1997;68:2227-2240.
Yang CS. Tea and health. Nutri 1999;15:946-949.
Sireesha K, Sailaja Rao P. Oxidative stress and diabetes: an overview. Asian J Pharm Clin Res 2015;8:15-19.
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