ANTIGENICITY OF HYDROLYZED RICE FORMULA IN ANIMAL MODEL (BALB/C MICE)

Authors

  • Amina Cherifa Brahim Laboratory of Nutrition physiology and food safety, Department of Biology, Faculty of Science, University of Oran, Algeria
  • Samia Addou Laboratory of Nutrition physiology and food safety, Department of Biology, Faculty of Science, University of Oran, Algeria
  • Amel Merzouk Laboratory of Nutrition physiology and food safety, Department of Biology, Faculty of Science, University of Oran, Algeria
  • Omar KHÉROUA Laboratory of Nutrition physiology and food safety, Department of Biology, Faculty of Science, University of Oran, Algeria
  • Djamel Saidi Laboratory of Nutrition physiology and food safety, Department of Biology, Faculty of Science, University of Oran, Algeria

Keywords:

Infantile Formula, Proteins, Antigenicity, Hydrolyzed rice

Abstract

Objective: The hydrolyzed formulas of bovine proteins used in the cow's milk allergy treatment contain peptides which can preserve their allergenicity. These last years, a new preparation with hydrolyzed rice proteins was marketed and could establish a useful alternative in the cases of cow's milk allergy.

The objective of our work is to study the biochemical characteristics of an infantile formula based on hydrolyzed rice and its antigenic effect on the Balb/c mice immunized with α-La protein.

Methods: In our work, we have realized an electrophoresis on polyacrylamide gel to determine various proteins which compose this infantile formula. Lowry's method is used to determine the amount of proteins in the formula. Our work has also allowed us to study the antigenicity of the hydrolyzed rice formula using ELISA method by the use of Balb/c mice serum.

Results: Our results have shown that

  • The electrophoresis analysis of the hydrolyzed rice formula has shown the absence of protein bands and consequently the lack of intact proteins in the formula.
  • The protein content of the hydrolyzed rice formula is close to the breast milk protein concentration which is adapted to the needs of the infant.
  • The hydrolyzed rice formula reacts very weakly with the anti α±-La IgG.

Conclusion: The commercial hydrolyzed rice formula can be adapted to cover the needs of the infant. This infantile formula was treated by technological methods to reduce the antigenic potential to prevent Allergy.

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References

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Published

01-09-2014

How to Cite

Brahim, A. C., S. Addou, A. Merzouk, O. KHÉROUA, and D. Saidi. “ANTIGENICITY OF HYDROLYZED RICE FORMULA IN ANIMAL MODEL (BALB/C MICE)”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 9, Sept. 2014, pp. 352-4, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/1963.

Issue

Vol 6, Issue 9, 2014

Section

Original Article(s)