MICROSCOPIC, PHYSICOCHEMICAL AND PHYTOCHEMICAL ANALYSIS OF GARDENIA JASMINOIDES (ELLIS)

Keerthana Kesavan; Jothi Gnanasekaran; Sridharan Gurunagarajan; Agnel Arul John Nayagam

doi:10.22159/ijpps.2018v10i1.21665

Authors

Keerthana Kesavan Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), No.7, Nelson Road, Thiruvanaikovil, Tiruchirappalli 620005. Tamil Nadu, India
Jothi Gnanasekaran Dean Sciences, Srimad Andavan Arts and Science College (Autonomous), No.7, Nelson Road, Thiruvanaikovil, Tiruchirappalli 620005 Tamil Nadu, India
Sridharan Gurunagarajan Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), No.7, Nelson Road, Thiruvanaikovil, Tiruchirappalli 620005. Tamil Nadu, India
Agnel Arul John Nayagam Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), No.7, Nelson Road, Thiruvanaikovil, Tiruchirappalli 620005 Tamil Nadu, India

DOI:

https://doi.org/10.22159/ijpps.2018v10i1.21665

Keywords:

Gardenia jasminoides, Microscopic, Physicochemical, Phytochemical

Abstract

Objective: To evaluate the microscopical, physicochemical and phytochemical analysis of Gardenia jasminoides (Ellis).

Methods: The leaves of Gardenia jasminoides (Ellis) was studied through macroscopic, microscopic, physicochemical standards such as moisture content, ash values, extractive values and phytochemical analysis includes preliminary screening and quantification of important secondary metabolites were carried out as per the standard procedures are given in Indian Ayurvedic Pharmacopeia.

Results: Microscopic studies of leaf showed the presence of epidermis, 4-5 layers of collenchymas cells, xylem, phloem and vascular bundles. The determination of physicochemical studies of leaves showed that it contains Total ash (6.98%), Water and Acid insoluble ash values and extractive values of 0.31% in Hexane, 0.87% in chloroform and 0.42% in Ethyl acetate. Preliminary phytochemical screening revealed the presence of Alkaloid, Flavonoid, Saponin, Tannin and phenol.

Conclusion: The results obtained from the study provide information for proper identification and standardization of plant for its medicinal property, ensuring the quality of plant drug and also useful to distinguish it from its related species.

Downloads

Download data is not yet available.

Author Biography

Keerthana Kesavan, Department of Biochemistry, Srimad Andavan Arts and Science College (Autonomous), No.7, Nelson Road, Thiruvanaikovil, Tiruchirappalli 620005. Tamil Nadu, India

Assistant Professor,

Department of Biochemistry,

Srimad Andavan Arts and Science College,

Tiruvanaikovil, Tiruchirappalli - 620 005

Tamilnadu, India.

References

Evans WC. Trease and Evans' Pharmacognosy. 15th edn. London: WB Saunders; 2001.

Barnes J, Anderson LA, Phillipson JD. Herbal medicine. 3rd Edition. Pharmaceutical Press: London; 2007. p. 1-23.

Nilakshi Pradhan, Jyoti Gavali, Nitin Waghmare. WHO (world health organization) guidelines for standardization of herbal drugs. International Ayurvedic Medical Journal; 2015.

Molony, David, Ming Ming Pan Molony. The American Association of Oriental Medicine's Complete Guide to Chinese Herbal Medicine. New York: Berkley Publishing; 1999.

Thitikornpong W, Phadungcharoen T, Sukrong S. Pharmacognostic evaluations of Lagerstroemia speciosa leaves. J Med Plant Res 2011;5:1330-7.

Anonymous. Indian Ayurvedic Pharmacopoeia. Vol. II. Controller of Publications. Edition 4th New Delhi: Government of India; 1996.

Anonymous. Quality Control Methods for Medicinal Plant Materials (An authorized publication of World Health Organization, Geneva). New Delhi: A. I. T. B. S. Publishers and Distributors (Regd.); 2002.

Brindha P, Sasikala, Bhima Rao. Pharmacognostic studies on Coleus Aromaticus. Benth. Indian Borage, B. M. E. B. R, Vol XII; 1991. p. 17-31.

Ferguson NM. A textbook of pharmacognosy, Mcmillan Company, New Delhi; 1956. p. 191.

Kadifkovapanovska T, Kulevanova S, Marina Stefova. In vitro antioxidant activity of some Teucrium species (Lamiaceae). Acta Pharm 2005;55:207-14.

Malick CP, Singh MB. Estimation of Phenol. In: Plant Enzymology and histoenzymology. New Delhi. Kalyani Publishers; 1980. p. 286.

The Ayurvedic Pharmacopoeia of India. Part II. (Formulations) Vol. II First Edition. The government of India, Ministry of Health and Family Welfare, AYUSH, New Delhi; 2008.

Pandita Nancy, Vaidya Ashlesha. Pharmacognostic and phytochemical studies of Cassia absus seed extracts. Int J Pharm Pharm Sci 2016;8:325-32.

Pravin Chandra PC. Medicinal Plants: Traditional Knowledge, IK International private limited; 2006. p. 1-8.

Kubmarawa D, Ajoku GA, Enworem NM, Okorie DA. Preliminary phytochemical and antimicrobial screening of 50 medicinal plants from Nigeria. Afr J Biotechnol 2007;6:1690-6.

Chaudhari M, Mengi S. Evaluation of phytoconstituents of Terminalia arjuna for wound healing activity in rats. Phytother Res 2006;20:799â€“805.

Carbanaro M, Mattera M, Nicoli S, Bergamo P, Cappelloni M. Modulattion of antioxidant compounds in organic vs conventional fruit (peach, Prunus persica and pear, Pyrus communis L.). J Agric Food Chem 2002;50:5458-62.

Koleckar V, Kubikova K, Rehakova Z, Kuca K, Jun D, Jahodar L. Condensed and hydrolysable tannins as antioxidants influencing the health. Mini Rev Med Chem 2008;8:436-77.

Jyoti Vandana, Gupta AK, Alok Mukerjee. Phytochemical screening and evaluation of anti-inflammatory activity of aerial parts extracts of plantago major L. Asian J Pharm Clin Res 2017;10:307-11.