HYPOGLYCEMIC ACTIVITY OF COPERNICIACERIFERA MART.L.EAF POWDER EXTRACT IN THE TREATMENT OF ALLOXAN-INDUCED DIABETIC MICE
Keywords:
Carnauba wax, Cinnamic acid esters, Diabetes MellitusAbstract
Objective: This study characterizeesters from carnauba crude powder and evaluates its hypoglycemic effect on the treatment of diabetic animals.
Methods: Groups of diabetic micewere induced by alloxan and treated with carnauba crude powder. Blood samples were collected to determine the glucose serum level.
Results: The compound was identified and characterized as diester of the 4-methoxycinnamic acid (PCO-C) and presented hypoglycemic effect in the concentrations of 100 and 150 mg/kgbody weight (b.w. )The concentration 150 mg/kg b.w..o.f PCO-C presented the best effect on controlling glucose levels (p<0.05),when compared to the reference drug.
Conclusion: The results indicate that the PCO-C is a promising therapeutic compound with hypoglycemic effect. This action can be justified by the presence of the diester of 4- methoxycinnamic acid.
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Shrivastava RBL, Shrivastava PS, Ramasamy J. Role of self-care in management of diabetes mellitus. J Diabetes Metabolic Disorders 2013;12(14):1-5.
Tabish SA. Is diabetes becoming the biggest epidemic of the twenty-first century? Int J Health Sci 2007;1(2):5-8.
Wild S, Roglic G, Green A. Global prevalence of diabetes. Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27(5):1047-53.
Harley E, Mirsky N, Ofir R. Antidiabeticatributes of desert and steppicplantts: a review. Planta Med 2013;79(6):425-36.
OkpuzorJ, Adebesin O, Ogbunugafor H, Amadi I. The potential of medicinal plants in sickle cell disease control: a review. Int J Biom Health Sci 2008;4(2):47-55.
Leila Z, Eliandra DS, Luisa HC, Anildo CJ. Effect of crude extract and fractions from Vitexmegapotamica leaves on hyperglycemia in alloxan diabetic rats. J Ethnopharmacol 2007;109(1):151-55.
Katiyar D, Singh B, Lall AM, Haldar C. Efï¬cacy of chitooligosaccharides for the management of diabetes in alloxan induced mice: a correlative study with antihyperlipidemic and antioxidative activity. Eur J Pharm Sci 2011;44(4):534-43.
Negri G. Diabetes melito: plantas e princÃpios ativos naturais hipoglicemiantes. Rev Bras Ciênc Farm 2005;41(2):121-42.
Dang KH, Singh Z, Swinny EE. Edible coatings Influence fruit ripening, Quality, and aroma biosynthesis in mango fruit. J Agric Food Chem 2008;56(4):1361-70.
Arias MA, Loxley A, Eatmon C, Roey GV, Fairhurst D, Mitchnick M, et al. Carnauba wax nanoparticles enhance strong systemic and mucosal cellular and humoral immune responses to HIV-gp140 antigen. Vaccine 2011;29(6):1258-69.
Barman K, Asrey R, Pal RK. Putrescine and carnauba wax pretreatments alleviate chilling injury, enhance shelf life and preserve pomegranate fruit quality during cold storage. Sci Hortic 2011;130:795-800.
Jo WS, Song HY, Song NB, Lee JH, Min SC, Song KB. Quality and microbial safety of ‘Fuji’ apples coated with carnauba-shellac wax containing lemongrass oil. LWT-Food Sci Technol 2014;55(2):490-97.
Ullmann F, Gerhartz W, Yamamoto YS, Campbell FT, Pfefferkorn R, Rounsaville JF. Ullmann´s Encyclopedia of Industrial Chemistry. Frankfurt, Germany: VCH-Verlagsgesellschaft;1996.
Hwanga KT, Cuppett SL, Wellerc LC, Hannad AM. HPLC of grain sorghumwax classes highlighting separation of aldehydes fromwax esters and steryl esters. J Sep Sci 2002;25(9):619-23.
Weitzel G, Fretzdorff AM, Stock W. Syntheses and hypoglycemic activities of ethyl esters and various amides of omega-guanidino fatty acids with medium chain length. H Oppe Seylers Z Physiol Chem 1980;361(1):41-50.
Malaisse W, Lebrun P. Mechanisms of sulfonylurea-lnduced insulin release. Diabetes Care 1990;13(3):9-17.
Vandenburg LE, Wilder EA. Aromatic acids of carnauba wax. J Am Oil Chem Soc 1967;44(11):659-62.
Vandenburg LE, Wilder EA. The structural constituents of carnauba wax. J Am Oil Chem Soc 1970;47(12):514-18.
Organization for Economic Cooperation and Development (OECD) guidelines for acute toxicity of chemicals. No. 425 (Adopted: 3 October 2008).
Jain DK, Arya RK. Anomalies in alloxan-induced diabetic model: it is better to standardize it first. Indian J Pharmacol 2011;43(1):91.
Misra M, Aiman U. Alloxan: an unpredictable drug for diabetes induction? Indian J Pharmacol 2012;44(4):538-39.
Pareek H, Sharma S, Khajja BS, Jain K, Jain GC. Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridaxprocumbens (Linn.). BMC Complement Altern Med 2009;9(48):1-7.
Kim JS, Godber JS, King J, Priyawiwatkul W. Inhibition of cholesterol autoxidation by the nonsaponifiable fraction in rice bran in an aqueous model system. J Am Oil Chem Soc 2001;78(7):685-9.
Fang N, Yu S, Badger TM. Characterization of triterpene alcohol and sterol ferulates in rice bran using LC-MS/MS. J Agric Food Chem 2003;51(11):3260-67.
Berthold HK, Unverdorben S, Degenhardt R, Bulitta M, Gouni-Berthold I. Effect of policosanol on lipid levels among patients with hypercholesterolemia or combined hyperlipidemia: a randomized controlled trial. Jama 2006;295(19):2262-9.
Castaño G, Mas R, Fernandéz R, Ilinait J, Gámez R, Alvarez E. Effects of policosanol 20 versus 40 mg/day in the treatment of patients with type II hypercholesterolemia: a 6-month double-blind study. Int J Clin Pharmacol Res 2001;21(1):43-57.
Son MJ, Rico CW, Nam SH, Kang MY. Effect of oryzanol and ferulic acid on the glucose metabolism of mice fed with a high-fat diet. J Food Sci 2011;76(1):7-10.
Justo ML, Rodriguez-Rodriguez R, Claro CM, Alvarez de Sotomayor M, Parrado J, Herrera MD. Water-soluble rice bran enzymatic extract attenuates dyslipidemia, hypertension and insulin resistance in obese Zucker rats. Eur J Nutr 2013;52(2):789-97.
Spiazzi CC, Manfredini V, Barcellos da Silva FE, Flores EM, Izaguirry AP, Vargas LM, Soares MB, Santos FW. γ-Oryzanol protects against acute cadmium-induced oxidative damage in mice testes. Food Chem Toxicol 2013;55:526-32.
Marongiu B, Piras A, Porcedda S. Comparative analysis of the oil and supercritical CO2 extract of Elettariacardamomum (L.) Maton. J Agric Food Chem 2004;52(20):6278-82.