PHARMACOGNOSTICAL AND PHARMACOLOGICAL EVALUATION OF CRINUM LATIFOLIUM L.

Puspendra Kumar Shukla; Manish Kumar; Ankita Misra; Bhanu Kumar; Ruchi Dwivedi; Sharad Srivastava

doi:10.22159/ijpps.2018v10i11.22968

Authors

Puspendra Kumar Shukla Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow-226001, (India)
Manish Kumar Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow-226001, (India)
Ankita Misra Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow-226001, (India)
Bhanu Kumar Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow-226001, (India)
Ruchi Dwivedi Mahatma Gandhi Chitrakoot Gramodaya University, Chitrakoot, Satna, M. P. 485334 (India)
Sharad Srivastava Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow-226001, (India)

DOI:

https://doi.org/10.22159/ijpps.2018v10i11.22968

Keywords:

Crinum latifolium, Antioxidant, Antidiabetic, DPPH, HPTLC

Abstract

Objective: Pharmacognostical study along with the development of a quantitative HPTLC method for Crinum latifolium and evaluation of its traditional claims.

Methods: Quantification of three marker compounds oleanolic acid, linoleic acid, and lupeol was done through HPTLC. In vitro antioxidant activity was determined by six different models, namely total phenolic and total flavonoid content, DPPH radical scavenging assay, ferric reducing power, antioxidant capacity and hydroxyl radical scavenging assay. In vitro antidiabetic activity was evaluated by α-amylase inhibition assay based on starch iodine and DNS method.

Results: The content of oleanolic acid, linoleic acid, and lupeol were found to be higher in aerial parts like 0.015%, 0.048%, and 0.028% respectively, while in root extract 0.006%, 0.027% and 0.025% respectively on a dry weight basis. Free radical scavenging activity was done by DPPH assay, showing the IC₅₀ value of 410±1.105 µg/ml in roots and 441.95±1.788 in aerial parts. In vitro antidiabetic potential of both the parts were assessed by starch iodine color assay and DNS method of alpha-amylase inhibition model. In 3,5 DNS assay, IC₅₀ of extract from aerial parts was 282.21±2.151µg/ml whereas in root extract it was 193.33±2.45µg/ml. Iodine-starch assay of C. latifolium (aerial part) shown the IC₅₀ value of 340.81±0.49 µg/ml and C. latifolium (root) of 74.64±1.28 µg/ml.

Conclusion: The results indicate that the aerial parts of the plant possess more antidiabetic potential in comparison to the root. Thus, the aerial part can be used to get better results as a drug and roots can be used as an alternative.

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Author Biography

Sharad Srivastava, Pharmacognosy Division, CSIR-National Botanical Research Institute, Lucknow-226001, (India)

Pharmacognosy Division
CSIR-NBRI

Principal Scientist

References

Ghosal S, Saini KS, Razdan S. Crinum alkaloids: their chemistry and biology. Phytochem 1985;24:2141-56.

Sainkhediya J, Ray S. Studies on the threatened ethnomedicinal plants used by tribals of harda district of MP. Int J Sci Res 2014;3:2590-3.

Kirktikar KR, Basu BD. Indian medicinal plants, international book distributors. Vol. I. Dehradun, India; 1987.

Tambekar DH, Dahikar SB. Antibacterial activity of some Indian Ayurvedic preparations against enteric bacterial pathogens. J Adv Pharm Tech Res 2011;2:24-9.

Tran DT, Do VT, Do TK, Pham TKB, Le NBR, Le PL. Assessment of therapeutic effect of soft gel Crinum latifolium for benign prostatic hypertrophy. Ministry of health bachs main hospital. Vietnam National Institute Of Gerontology; 2005. p. 14-6.

Ghosal S, Saini KS, Frahm AW. Alkaloids of Crinum latifolium. Phytochem 1983;22:2305-9.

Kokate CK. Practical pharmacognosy. Vallabh Prakashan, New Delhi; 2010. p. 4:17-26.

Koparde A, Magdum C. Phytochemical studies and pharma-cognostical evaluation of Zingiber cassumunar Roxb. Asian J Pharm Clin Res 2017;10:129-35.

Bray HG, Thorpe WV. Analysis of phenolic compounds of interest in metabolism. Methods Biochem Anal 1954;1:27-52.

Narayan S, Mittal A. In vitro antioxidant activity and free radical scavenging potential of roots of red sage. Asian J Pharm Clin Res 2015;8:417-21.

Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 1995;28:25-30.

Oyaizu M. Studies on products of browning reaction. Japan J Nutr 1986;44:307-15.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.

Kunchandy E, Rao MN. Oxygen radical scavenging activity of Curcumin. Int J Pharma 1990;58:237-40.

Xiao Z, Storms R, Tsang A. A quantitative starch? Iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem 2006;351:146-8.

Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 1959;31:426-8.

Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM, Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci Res Essay 2007;2:163-6.