INHIBITION OF TUMOUR NECROSIS FACTOR (TNF) ACTIVITY BY A NEW ERGOSTANE STEROID FROM CLEOME DROSERIFOLIA
DOI:
https://doi.org/10.22159/ijpps.2018v10i5.24945Keywords:
Cleome, Droserifolia, Ergosteroid, Dodecanol, TNF inhibitor, Autoimmune diseaseAbstract
Objective: To isolate steroidal compound from Cleome droserifolia and explore its activity against tumour necrosis factor (TNF) using molecular docking studies.
Methods: Extraction of the plant material was carried using two successive solvents and three compounds were isolated using column chromatography. The isolated compounds were identified using different spectroscopic data such as 1D, 2D nuclear magnetic resonance (NMR), ultraviolet-visible spectroscopy (UV-Visible), fourier transform infrared spectroscopy (FT-IR) and electrospray ionization mass spectroscopy (ESI-MS). Molecular docking was carried in order to investigate the activity of the ergostane derivative as tumour necrosis factor inhibitor.
Results: Two steroidal compounds were isolated and identified as ergost-5,7,9,24 (28)-tetraen-3-one (1) and β-sitosterol glucoside (3) by means of spectroscopic measurement. 1-dodecanol (2) was also isolated. The ergostane derivative showed several binding sites with TNF-α
Conclusion: The new ergostane derivative isolated from the aerial parts of Cleome droserifolia is introduced to serve as a potential tumour necrosis factor inhibitor and may be used as a lead compound for the treatment of rheumatoid arthritis.
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