CYTOTOXICITY OF METABOLITES PRODUCED BY ENDOPHYTIC FUNGUS CLADOSPORIUM SP. ISOLATED FROM MARINE MACROALGAE ON IN­VITRO MCF­7, HELA, AND DU-145 CELL LINES

Authors

  • Asri Peni Wulandari Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • R. R. Indry Noviarin Examinati Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • Madihah . Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • Desi Harneti Putri Huspa Department of Chemistry, Faculty of Mathematics and Natural Sciences Padjadjaran University
  • Poniah Andayaningsih Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • Poniah Andayaningsih

DOI:

https://doi.org/10.22159/ijpps.2018v10i8.25181

Keywords:

Cytotoxicity, Cladosporium sp, Endophytic, Fungus, MTT assay

Abstract

Objective: To investigate the in vitro cytotoxicity effect of the crude ethyl acetate extract of Cladosporium sp. on MCF-7, HeLa, and DU-145 cell lines.

Methods: In vitro cytotoxicity was evaluated by tetrazolium reduction assay. The percentage of cell inhibition was analyzed using probit analysis to obtain 50% inhibitory concentration (IC50). Morphological alteration of the cell lines after exposure with extract was observed under an inverted microscope.

Results: The ethyl acetate extract of the metabolite performed an anticancer activity for cancer cell line MCF-7, HeLa, and DU-145 with IC50 respectively 8.46 μg/ml; 9.87 μg/ml; and 98.03 μg/ml. The extract shows greater the anticancer activity and has strong antiproliferative on MCF-7 and HeLa cell line than DU-145. Confirmation morphological were observed under the inverted microscope showed a morphological change in cancer cells when incubated with the extract.

Conclusion: From the performed assay, the crude extract of Cladosporium sp. exhibit cytotoxic activity against MCF-7, HeLA, and DU-145.

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Published

01-08-2018

How to Cite

Wulandari, A. P., R. R. I. N. Examinati, M. ., D. H. P. Huspa, P. Andayaningsih, and P. Andayaningsih. “CYTOTOXICITY OF METABOLITES PRODUCED BY ENDOPHYTIC FUNGUS CLADOSPORIUM SP. ISOLATED FROM MARINE MACROALGAE ON IN­VITRO MCF­7, HELA, AND DU-145 CELL LINES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 10, no. 8, Aug. 2018, pp. 72-76, doi:10.22159/ijpps.2018v10i8.25181.

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