EVALUATION OF ANTI-HYPERTROPHIC POTENTIAL OF CAMELLIA SINENSIS IN ISOPROTERENOL INDUCED CARDIAC HYPERTROPHY
DOI:
https://doi.org/10.22159/ijpps.2018v10i10.28101Keywords:
Camellia sinensis, Isoproterenol, Cardiac hypertrophyAbstract
Objective: Camellia sinensis (C. sinensis family-Theaceae) has potent antioxidant activity used in the treatment of cardiovascular disease. The present study evaluates the cardioprotective (anti-hypertrophic) effect of aqueous extract of C. sinensis in isoproterenol (ISO) induced cardiac hypertrophic rats.
Methods: The beneficial effect of the green tea extract was examined by the administration of the aqueous extract of the leaves of C. sinensis (100 mg/kg b.w., oral., 7 d) in ISO (10 mg/kg b.w., subcutaneous.,7 d) induced cardiac hypertrophic rats with reference to the standard drug, losartan (50 mg/kg b.w., oral.,7 d) followed by biochemical estimations of glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and lactate dehydrogenase (LDH) in serum and heart tissues thus collected at the end of 7 d.
Results: The biochemical assays revealed significantly (P<0.05) increased glucose, protein, cholesterol, cardiac marker enzymes namely serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) lactate dehydrogenase (LDH) and significantly (P<0.05) decreased in ISO induced cardiac hypertrophic rats that were reciprocated by the effect of plant extract.
Conclusion: Thus, this study showed that the aqueous leaf extract of C. sinensis possesses potent effect against cardiac hypertrophy. This potential is hypothesized to be due to the phytochemical, Catechin present in the plant that requires further isolation and characterization with respect to anti-hypertrophic therapeutics.
Downloads
References
Frey N, Katus HA, Olson EN, Hill JA. Hypertrophy of the heart: a new therapeutic target. Circulation 2004;109:1580-9.
Carreno JE, Apablaza F, Ocaranza MP, Jalil JE. Cardiac hypertrophy: molecular and cellular events. Rev ESP Cardiol 2006;59:473-86.
Bopanna KN, Kannan J, Godgil S, Balaraman R, Rathod SP. Antidiabetic and Antihyperlipidemic effects of Neem seed kernel powder on alloxan diabetic rabbits. Indian J Pharmacol 1997;29:162-72.
Chu DC, Juneja LR. General chemical composition of green tea and its infusion. In: Juneja LR, Chu DC, Kim M. [Eds] Chemistry and application of green tea. CRC press, Boca Raton; 1997. p. 13-22.
Gramza A, Korczak J. Tea constituents (Camellia sinensis L.) as antioxidants in lipid systems. Trends Food Sci Tech 2005;16:351-8.
Hara Y. Green tea: health benefits and applications. Bola Raton, FL. CRL Press: Taylor and Francis group; 2001. p. 252.
Cabrera C, Artacho R, Gimenez R. Beneficial effects of green tea: a review. J America College Nutr 2006;25:79-99.
Nie G, Cao Y, Zhao B. Protective effects of green tea polyphenols and their major components, (-). Epigallocatechin-3-gallate (EGCG), on 6-hydroxydopamine-induced in PC 12 cells. Redox Rep 2002;7:171-7.
Huang Q, Wu LJ, Tashiro S, Gao HY, Onodera S, Ikejima T. (+) catechin, an ingredient of green tea, protects murine microglia from Oxidative stress-induced DNA damage and cell cycle arrest. J Pharmacol Sci 2005;98:16-24.
Schuppan DJ, Jia B, Brikhaus EG, Hahn. Herbal products for liver disease: a therapeutic challenge for the new millennium. Hepatology 1999;30:1099-104.
Tsuneki H, Ishizuk M, Terasawa M, Jin-Bi W, Sasaoka T, Kimura I. Effect of green tea on blood glucose levels and serum proteomic patterns (db/db) mice and on glucose metabolism in healthy humans. BMC Pharmacol 2004;26:18-28.
Chisaka T, Matsuda H, Kubomura Y, Mochizuki M, Yamahara J, Fujimura H. The effect of crude drugs on experimental hypercholesterolemia: mode of action by (-) epigallocatechin gallate in tea leaves. Chem Pharm Bull 1998;36:227-33.
Arakawa H, Maeda M, Shimamura T. Role of hydrogen peroxide in bactericidal action of catechin. Biol Pharm Bull 2004;27:277-81.
Anwar E, Utami TD, Ramadon D. Transfersomal gel containing green tea (Camellia sinensis L. Kuntze) leaves extract: increasing in vitro penetration. Asian J Pharm Clin Res 2017;10:1-5.
Permatasari IA, Jufri M. Formulation of nicosomal gel containing green tea extract (Camellia sinensis L. Kuntze) using thin-layer hydration. Int J Appl Pharm 2017;9 Suppl 1:38-43.
Murali B, Upadhyaya UM, Goyal RK. Effect of chronic treatment with Enicostemma littorale in non-insulin dependent diabetic (NIDDM) rats. J Ethnopharmacol 2002;81:199-204.
Zhang S, Tang F, Yang Y, Lu M, Luan A, Zhang J, et al. Astragaloside IV protects against isoproterenol-induced cardiac hypertrophy by regulating NF-κB/PGC-1α signaling mediated energy biosynthesis. Plosone 2015;1-18. https://doi.org/10.1371/journal.pone.0118759
Shimada JY, Passeri JJ, Baggish LA, Callaghan OC, Lowry PA, Yannekis G, et al. Effects of losartan on left ventricular hypertrophy and fibrosis in patients with nonobstructive hypertrophic cardiomyopathy. JACC Heart Fail 2013;1:480-7.
Upaganlawar A, Gandhi C, Balaraman R. Effect of green tea and Vitamin E combination in isoproterenol induced myocardial infarction in rats. Plant Foods Human Nutr 2009;64:75-80.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin-phenol reagents. J Biol Chem 1951;193:265-75.
Kakkar P, Das B, Vishwanathan PN. A modified spectro-photometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130–2.
Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.
Rotruck JT, Pope AL, Ganther HE, Swasson AS, Haseman DG, Howkstra WG. Selenium: biochemical role as a component of glutathione peroxidase. Science 1973;779:588–90.
Yang J, Chen NY, Xu XZ, Mou Y, Zheng RL. Alteration of RhoA prenylation ameliorates cardiac and vascular remodeling in spontaneously hypertensive rats. Cell Physiol Biochem 2016;39:229-41.
Al-Rasheed MN, Al-Oteibi MM, Al-Manee ZR, Al-Shareef AS, Al-Rasheed MN, Hasan HI, et al. Simvastatin prevents isoproterenol-induced cardiac hypertrophy through modulation of the JAK/STAT pathway. Drug Design Development Ther 2015; 9:3217-29.
Hamada M, Shigematsu Y, Ohtani T, Ikeda S. Elevated cardiac enzymes in hypertrophic cardiomyopathy patients with heart failure. J Japanese Circulation Soc 2016;80:218-26.
Shen XC, Qian ZY. Effects of crocetin on antioxidant enzymatic activities in cardiac hypertrophy induced by norepinephrine in rats. Pharmazie 2006;61:348-52.
Published
How to Cite
Issue
Section
