EVALUATION OF PHYTASE PRODUCTION BY HYPOCREA LIXII SURT01 IN SUBMERGED AND SOLID-STATE FERMENTATIONS
Keywords:
Phytase, Submerged fermentation, Solid state fermentation, Enzyme activityAbstract
Objective: Phytases have important applications in human and animal nutrition because they hydrolyze the phytate present in legumes, cereal grains and oil seeds to release inorganic phosphate. Supplementation of phosphate to the poultry causes a serious problem of eutrophication. This can be reduced by incorporating phytase in poultry feed. Present study explains extracellular phytase production by SmF and SSF from a fungal strain Hypocrea lixii SURT01.
Methods: Extracellular phytase production by Hypocrea lixii SURT01 was evaluated in media containing various refined carbon sources (Fructose, Sucrose, Maltose and lactose in concentration ranging from 1.5% to 7.5%) along with standard medium under submerged fermentation (SmF). At the same time, phytase production was studied under Solid State Fermentation (SSF) with four different substrate such as barley, green gram, bengal gram and black gram.
Results: In SmF out of different carbon sources in various concentrations, 6% sucrose showed maximum enzyme production (245U/ml). In SSF, barley showed highest phytase yield (1638 Units/ml) on 5th day of incubation.
Conclusion: Evaluation of Solid state fermentation showed enhanced phytase production when compared to Submerged Fermentation.
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References
Raboy V. myo-Inositol-1, 2, 3, 4, 5, 6-hexakisphosphate. Phytochemistry 2003;64:1033-43.
Pallauf J, Rimbach G. Effect of Supplementation Phytase on Mineral and Trace Element Bioavailability and Heavy Metal Accumulation in Pigs with Different Type of Diets. In: Coelho MB, Kornegay ET, editors. Phytase in Animal Nutrition and Waste Management. Mount Olive, New Jersey: BASF; 1996. p. 451–65.
Reddy NR, Pierson MD, Sathe SK, Salunkhe DK. Phytates in cereals and legumes. CRC Press: Inc., Boca Raton, Fla; 1989. p. 152.
Davies NT. Effects of phytic acid on mineral availability. In: Vahoung GV, Kritchevsky K (eds). Dietary Fiber in Health and Disease. Plenum Press: NY, USA 1982;99:105–16.
Pitcher DG, Saunders NA, Owen RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989;8:151–6.
Thyagarajan R, Karthick Raja Namasivayam S, Narendrakumar G. Optimization of medium components for phytase production byhypocrealixii surt01 using response surface methodology. J Pure Appl Microbiol 2014;8(3):2485-90.
Soni SK, Khire JM. Production and partial characterization of two types of phytase from Aspergillus niger NCIM 563 under submerged fermentation conditions. World J Microbiol Biotechnol 2007;23:1585-93.
Shieh TR, Ware JH. Survey of microorganisms for theproduction of extracellular phytase. Appl Microbiol 1968;16:1348-51.
RodrÃguez-Fernández DE. Recovery of phytase produced by solid-state fermentation on citrus peel. Braz Arch Biol Technol 2010;53(6):1487-96.
Tahir A, Mateen S, Saeed Uslu. Studies on the Production of commercially important Phytase from Aspergillusniger ST-6 isolated from decaying organic soil. Int J Mushroom Sci 2010;22(2):51-7.
Ebune A, Al-Asheh S, Duvnjak Z. Effects of phosphate, surfactants and glucose on phytase production and hydrolysis of phytic acid in canola meal by Aspergillusficuum during solid-state fermentation. Bioresour Technol 1995;53:7–12.
Heinonen JK, Lahti RJ. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase. Anal Biochem 1981;113:313-7.
Lowry OH, Rosebrough NJ, Farr AL, Randall RL. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
Prasantha Kumari M, Sudheer Kumar M, Nageswara Rao G. Isolation of phytase producing fungi and optimization of production parameters. J Global Trends Pharm Sci 2011;2(2):161-76.
Spier MR, Greiner R, RodrÃguez-León JA, Woiciechowski AL, Pandey A, SoccolVT, et al. Phytase production using citric pulp and other residues of the agro-industry in SSF by fungal isolates. Food Technol Biotechnol 2008;46:178-82.
Ngo Thanh Xuan, Mai Thi Hang, Vu Nguyen Thanh. Cloning and over Expression of an Aspergillus niger XP Phytase Gene (phyA) in Pichia pastoris World Academy of Science, Eng Technol 2009;3:693-6.
Chen CC, Wu PH, Huang CT, ChengKJ. A Pichia pastoris fermentation strategy for enhancing the heterologous expression of an Escherichiacoli phytase. Enz Microbial Technol 2004;35:315-20.