SCREENING OF PHYTOCHEMICAL, ANTIOXIDANT ACTIVITY AND ANTI-BACTERIAL ACTIVITY OF MARINE SEAWEEDS
DOI:
https://doi.org/10.22159/ijpps.2019v11i1.29119Keywords:
Seaweed, DPPH, Protein, Carbohydrate, Dictyopteris delicatula, Padina gymnospora, Acanthophora spicifera, Portieria hornemannii, Ulva faciataAbstract
Objective: Phytochemical is naturally present in the seaweeds which biologically play a significant role. The intention of this study was designed to screen the phytochemical constituents and antimicrobial potential of selected seaweed collected from Rameshwaram and Tuticorin Southern coast of India.
Methods: The present study investigated the presence of phytochemical constituents and also total phenol, total carbohydrate and total protein quantity of the brown seaweed. Dictyopteris delicatula, Padina gymnospora, Acanthophora spicifera, Portieria hornemannii and Ulva faciata were extracted with solvents having different polarities like methanol, ethanol, chloroform and water and screened for the phytochemical constituents, total phenol, total carbohydrate, total protein and DPPH with standard procedure. The antibacterial activities of the seaweeds were examined by agar well diffusion method.
Results: Among the five seaweeds, U. faciata showed the maximum number of active constituents in the methanol extract likewise P. gymnospora was found to have a number of diligent compounds in ethanol extract. A. spicifera showed minimum compounds in ethanol as well as chloroform extract. Moreover A. spicifera, P. hornemannii have shown the superior quantity of protein and carbohydrate when compared to other species. The scavenging activity of methanol extracts at 5 mg/ml concentration P. hornemannii shows 18.2% and A. spicifera possess 17.1%. In the antibacterial activity, methanol extracts of all the seaweed showed a potential inhibitory activity against B. cereus and P. aeruginosa compared to other pathogens.
Conclusion: The crude extract of seaweed manifest preferable antimicrobial and antioxidant activities, hence in the future, it would be good if it is further taken for treatment of human diseases or as new antimicrobial agents to replace synthetic antimicrobial agents.
Downloads
References
Carte BK. Biomedical potential of marine natural products: marine organisms are yielding novel molecules for use in basic research and medical applications. Bioscience 1996;46:271-86.
Ahilan B, Sujathkumar NV. Seaweed is it really useful. Seafood Export J 1990;22:23-5.
Bharathan G. Experimental culture of Gracilaria at the mariculture centre, Muttukadu, Tamil Nadu. J Mar Biol Assoc India 1987;29:54-9.
Burrows EM. Seaweeds of the British Isles: Chlorophyta. Nord J Bot; 1991.
Mishra VK, Temelli F, Ooraikul B, Shacklock PF, Craigie JS. Lipids of the red alga Palmaria palmate. Bot Mar 1993;6:169-74.
Rizvi M, Shameel M. Studies on the bioactivity and elementology of marine algae from the coast of Karachi, Pakistan. Phytother Res 2004;18:865-72.
Hameed A, Vohra S. Indian system of medicine skin diseases (A herbo mineral Approach), New Delhi, India. CBS Publishers 2001. p. 174.
Keyrouz R, Abasq M, Le Bourvellec C, Blanc N, Audibert L, ArGall E, et al. Total phenolic contents, radical scavenging and cyclic voltammetry of seaweeds from Brittany. Food Chem 2011;126:831-6.
RodrÃguez Montesinos YE, Arvizu Higuera DL, Hernandez Carmona G. Seasonal variation on size and chemical constituents of Sargassum sinicola setchellet gardner from bahÃa de la paz, baja california sur. Mexicol Phycol Res 2008;56:33-8.
Tulay A, Fusun S. Rıza antimicrobial activities of some marine algae and some cyanobacteria from çanakkale (Turkey). J Algal Biomass Utilisation Br J Pharmacol 2013;4:35-40.
Demirel Z, Yilmaz Koz FF, Karabay Yavasoglu UN, Ozdemir G, Sukatar A. Antimicrobial and antioxidant activity of brown algae from the Aegean Sea. J Serb Chem Soc 2009;74:619-28.
Chakraborty C, Hsu CH, Wen ZH, Lin CS, Agoramoorthy G. Zebrafish: a complete animal model for in vivo drug discovery and development. Curr Drug Metabol 2009;10:116-24.
Chandran M, Vivek P, Kesavan D. Phytochemical screening and anti-bacterial studies in salt marsh plant extracts (Spinifexlittoreus (BURM. F) MERR. and Heliotropium curassavicum L.). Int J Chemtech Res 2014;6:4307-11.
Indumathi C, Durgadevi G, Nithyavani S, Gayathri PK. Estimation of terpenoid content and its antimicrobial property in Enicostemmalitorrale. Int J ChemTech Res 2014;6:4264-7.
Hassan A, Hawa S, Bakar A, Fadzelly M. Antioxidative and anticholinesterase activity of Cyphomandrabetacea fruit. Sci World J 2013:7. http://dx.doi.org/10.1155/2013/278071
Singh D, Singh P, Gupta A, Solanki S, Sharma E, Nema R. Qualitative estimation of the presence of a bioactive compound in Centella Asiatica: an important medicinal plant. Int J Life Sci Med Sci 2012;2:4-7.
Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Ghaffar R. Phytochemical analysis of medicinal plants occurring in local area of Mardan. Biochem Anal Biochem 2013;2:1-4.
National Committee for Clinical Laboratory Standards. Reference method for broth dilution antifungal susceptibility testing of yeast. Approved standard M27-A. PA: National Committee for Clinical Laboratory Standards Wayne; 1997.
Magaldi S, Mata Essayag S, De Capriles CH, Perez C, Colella MT, Olaizola C, et al. Well diffusion for antifungal susceptibility testing. Int J Infect Dis 2004;8:39-45.
Wang T, Jonsdottir R, Olafsdottir G. Total phenolic compounds, radical scavenging and metal chelation of extracts from Icelandic seaweeds. Food Chem 2009;116:240-8.
Wang X, Wu Y, Chen G, Yue W, Liang Q, Wu Q. Optimisation of ultrasound-assisted extraction of phenolic compounds from sparganiirhizoma with response surface methodology. Ultrason Sonochem 2013;20:846-54.
Dodgson KS, Price RG. A note on the determination of the ester sulphate content of sulphated polysaccharides. Biochem J 1962;84:106.
Yaphe W. Colorimetric determination of 3, 6-anhydrogalactose and galactose in marine algal polysaccharides. Anal Chem 1960;32:1327-30.
Roy S, Anantharaman P. Biochemical compositions of seaweeds collected from Olaikudaand Vadakkadu, Rameshwaram, Southeast Coast of India. J Marine Sci Res Dev 2017;7:2.
Dharmesh R, Stalin K, Ramavatar M, Siddhanta AK. Antioxidant activity and phytochemical analysis of a few Indian seaweed species. Indian J Geomarine Sci 2014;43:507-18.
Gomathi K, Anna Sheba L. Phytochemical screening and heavy metal analysis of Ulva reticulata. Asian J Pharm Clin Res 2018;11:84-8.
Nguyen VT, Ueng JP, Tsai GJ. Proximate composition, total phenolic content, and antioxidant activity of seagrape (Caulerpalentillifera). J Food Sci 2011;6:950-8.
Dhargalkar VK. Biochemical studies in Ulva reticulate Forskal. Mahasagar 1986;19:45-51.
Parthiban C, Saranya C, Girija K, Hemalatha A, Suresh M, Anantharaman P. Biochemical composition of some selected seaweeds from Tuticorin coast. Adv Appl Sci Res 2013;4:362-6.
Dinesh G, Sekar M, Kannan R. Nutritive properties of seaweeds of gulf of Mannar, Tamil Nadu. Seaweed Res Utiln 2007;29:125-32.
Selvi M, Shakila P, Selvaraj R. Studies on biochemical contents of macro-algae from cuddalore and Thirumullaivasal estuaries of Tamil Nadu. Seaweed Res Utiln 1999;21:99-103.
Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.
Baskaran K, Pugalendi KV, Saravanan R. Free radical scavenging activity of ethyl acetate extract of cardiospermum halicacabum by in vitro assays. Am J Mol Biol 2014;2:300-8.
Revathi D, Baskaran K, Subashini R. Antioxidant and free radical scavenging capacity of red seaweed Hypneavalentiae from Rameshwaram coast Tamil Nadu, India. Int J Pharm Pharm Sci 2015;8:232-7.
Alves MG, Dore CM, Castro AJ, do Nascimento MS, Cruz AK, Soriano EM, et al. Antioxidant, cytotoxic and hemolytic effects of sulfatedgalactans from edible red alga Hypneamusciformis. J Appl Phycol 2012;24:1217-27.
Priyadharshini S, Bragadeeswaran S, Prabhu K, Ran SS. Antimicrobial activity and hemolytic activity of seaweed extracts Ulva fasciata (Delile 1813) from Mandapam, Southeast coast of India. Asian Pacific J Trop Biomed 2011;1:S38-S39.
Lavanya R, Veerappan N. Antibacterial potential of six seaweeds collected from gulf of mannar of Southeast Coast of India. Adv Biol Res 2011;5:38-44.
Devi GK, Manivannan K, Thirumaran G, Rajathi FAA, Anantharaman P. In vitro antioxidant activities of selected seaweeds from the southeast coast of India. Asian Pacific J Trop Med 2011;4:205-11.
De Campos Takaki GM, Diu MB, Koening ML, Pereira EC. Screening of marine algae from Brazilian northeastern coast for antimicrobial activity. Bot Mar 1988;31:375-8.
Lavanya R, Fazina M, Kajal C, Lokanatha V. Phytochemical evaluation and antimicrobial activity of Gracilaria opuntia: an important anti-diabetic red marine macroalgae. Int J Curr Pharm Res 2017;9:37-41.
Published
How to Cite
Issue
Section
