POTENTIAL ANTIMICROBIAL, ANTHELMINTIC AND ANTIOXIDANT ACTIVITIES OF MYRISTICA DACTYLOIDES GAETRN BARK

Authors

  • Ajish A. D. Research Scholar, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.
  • Vagdevi H. M. Associate Professor, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.
  • Asha K. Research Scholar, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.
  • Jayanna N. D. Research Scholar, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.

Keywords:

Myristica dactyloides Gaetrn, Antimicrobial, Anthelmintic, Antioxidant, DPPH and Pheretima Posthuma

Abstract

Objective: The present study was undertaken to determine the antimicrobial, anthelmintic and antioxidant activities of bark extracts of Myristica dactyloides Gaetrn.

Methods: The antimicrobial activity of the petroleum ether, ethyl acetate and methanol extracts were evaluated by the Agar well diffusion method against different gram-positive, gram-negative bacteria and fungi. Different extracts of the plant were taken for anthelmintic activity against Indian earthworm Pheretima Posthuma. DPPH radical scavenging activity was measured by the DPPH antioxidant assay method using ascorbic acid as standard and the total phenolic content was estimated spectrophotometrically using Folin-Ciocalteu method.

Results: Petroleum ether extract exhibited significant antifungal activity, anthelmintic activity and considerable DPPH radical scavenging activity with an IC50 value of 10.97±0.07µg/ml. Whereas methanol extract exhibited significant antibacterial activity against both gram positive and gram negative bacteria and it is the richest source of phenolics with a total phenolic content of 95.11±2.14 mg of Catechol equivalents/100 mg dried extract. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, tannins/phenolics, steroids/triterpenoids and saponins which may be the reason for its biological properties.

Conclusion: The findings of this study indicate that this plant is medicinal with prominent antioxidant, antimicrobial and anthelmintic property. The plant can be considered as promising plant species with high potential value for drug preparation.

 

Downloads

Download data is not yet available.

Author Biographies

Ajish A. D., Research Scholar, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.

Department of Chemistry

Vagdevi H. M., Associate Professor, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.

Department of Chemistry

Asha K., Research Scholar, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.

Department of Chemistry

Jayanna N. D., Research Scholar, Department of Chemistry, Sahyadri Science College(Autonomous), Kuvempu University, Shivamogga.

Department of Chemistry

References

Oke JM, Hamburger MO. Screening of some Nigerian medicinal plants for antioxidant activity using 2, 2, diphenyl-picryl-hydrazyl radical. Afr J Biomed Res 2002;5:77-9.

Okeke IN, Laxminarayan R, Bhutta ZA. Antimicrobial resistance in developing countries. Part 1: recent trends and current status. Lancet Infect Dis 2005;5:481-93.

Abu-Shanab B, Adwan G, Abu-Safiya D. Antibacterial activities of some plant extracts used in Palestine in popular medicine. Turk J Biol 2004;28:99-102.

Nunomura RCS, DaSilva ECC, Oliverira DF, Garcia AM, Boeloni JN, Nunomura SM, et al. In vitro studies of the anthelmintic activity of Picrolemma spruce Hook. f. (Simaroubaceae). Acta Amazonica 2006;36(3):327-30.

Gutteridge J. Free radicals in disease processes: a compilation of cause and consequence. Free Radical Res 1993;19:141-58.

Knight JA. Diseases related to oxygen derived free radicals. Ann Clin Laboratory Sci 1995;25(2):111-21.

Rechner AR, Kuhnle G, Bremmer P, Hubbard GP, Moore KP, Rice Evans CA. The metabolic fate of dietary polyphenols in humans. Free Radical Biol Med 2002;33:220-35.

Kirtikar KR, Basu BD. Indian Medicinal plant. Valley offset publishers: New Delhi; 2006. p. 1037.

Bamidele O, Akinnuga AM, Alagbonsi IA, Ojo OA, Olorunfemi JO, Akuyoma MA. Effects of ethanolic extract of Myristica fragrans Houtt. (nutmeg) on some heamatological in albino rats. Int J Med Med Sci 2011;3(6):215-8.

Tan KP, Khoo HE, Azrina A. Comparison of antioxidant components and antioxidant capacity in different parts of nutmeg (Myristica fragrans). Int Food Res J 2013;20(3):1049-52.

Preedy VR. Beer in health and disease prevention. UK: Elsevier Inc; 2009.

Carlsen MH, Halvorsen BL, Holte K, Bohn SK, Dragland S, Blomhoff R. The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide. Nutr J 2010;9(3):1-11.

Trease GE, Evans WC. A Textbook of Pharmacognosy. 11thedition. Bailliere Tidall, London; 1978. p. 530.

Kokate CK, Purohith AP, Gokhale SB. Pharmacognosy. Nirali Prakashan, Pune; 1990. p. 120.

Thippeswamy B, Naveenkumar KJ, Guruprasad Bodharthi J, Shivaprasad SR. Antimicrobial activity of ethanolic extract of Usnea longissima. J Exp Sci 2011;2(12):1-3.

Shahi SK, Patra M, Dikshit A, Upreti DK. Parmelia cirrhatum: A Potential source of broad spectrum natural antifungal. National Botanical Research Institute. Lucknow, India; 2001.

Ajaiyeoba EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of B. coriaceae and G. gynandra extract. Pharm Biol 2001;39:217-20.

Wong SP, Lai PL, Jen HW. Antioxidant activities of aqueous extracts of selected plant. Food Chem 2006;99:775-83.

Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat Protoc 2007;2(4):875-7.

Bate Smith EC. The phenolic constituent of plants and their taxonomic significance, dicotylendons. J Linn Soc London Bot 1962;58:95-103.

Cuendet M, Hostettmann K, Potterat O. Helvetica Chimica Acta 1997; 80:1144–52.

Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res 2000;14:323-8.

MacDonald-Wicks LK, Wood LG, Garg ML. Methodology for the determination of Biologycal antioxidant capacity in vitro a review. J Sci Food Agric 2006;86:2046-56.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25-30.

Mensor LL, Menezes FS, Leitao GG, Reis AS, Dos Santos TC, Coube CS, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 2001;15:127-30.

Agarwal PK. Carbon-13 NMR of flavonoids. Elsevier, New York; 1989.

Pods edek A. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT Food Sci Technol 2007;40:1-11.

Jurd L, Geissmao TA. Absorption spectra of metal complexes of flavonoid compounds. J Org Chem 1956;21:1395-401.

Aqil F, Ahmed I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants: Turk J Biol 2006;30:177-8.

Shahidi F, Wanasundara PK. Phenolic antioxidants. Crit Rev Food Sci Nutr 1992;32:67-103.

Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5(11):1142-5.

Vinay R Patel, Prakash R Patel, Sushil S Kajal. Antioxidant activity of some selected medicinal plants in the Western Region of India. Adv Biol Res 2010;4(1):23-6.

Published

01-05-2015

How to Cite

A. D., A., V. H. M., A. K., and J. N. D. “POTENTIAL ANTIMICROBIAL, ANTHELMINTIC AND ANTIOXIDANT ACTIVITIES OF MYRISTICA DACTYLOIDES GAETRN BARK”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 5, May 2015, pp. 117-22, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/2949.

Issue

Vol 7, Issue 5, 2015

Section

Original Article(s)