SEPARATION AND ANALYSIS OF AMLODIPINE/BENAZEPRIL COMBINATION IN CAPSULES BY A NOVEL ION PAIR LIQUID CHROMATOGRAPHY

Saleh Trefi; Yaser Bitar

doi:10.22159/ijpps.2019v11i1.29672

Authors

Saleh Trefi Quality of Medicines and Pharmaceutical Chemistry Department-Faculty of Pharmacy-University of Aleppo-Syria
Yaser Bitar Quality of Medicines and Pharmaceutical Chemistry Department-Faculty of Pharmacy-University of Aleppo-Syria

DOI:

https://doi.org/10.22159/ijpps.2019v11i1.29672

Keywords:

HPLC, Ion pair liquid chromatography, Amlodipine, Benazepril

Abstract

Objective: The objective of this study was to develop and validate a novel ion-pair liquid chromatography method, in order to separate and assay of amlodipine/benazepril combination in capsules. This method was a fast, practical and additional choice in quality control laboratories.

Methods: The chromatographic conditions comprised of a classical C₁₈-type stationary phase (250 × 4.6 mm, 5μ), with a mobile phase consisting of: 45% of 10^-³ M of cetrimide and 55% acetonitrile. The flow rate was 1 ml/min; the detection wavelength was at 242 nm, under ambient temperature.

Results: The method was validated for linearity with correlation coefficients very close to one, the accuracy with mean recovery values between 95.0-105.0%, precision with relative standard deviations of the calculated concentrations less than 5.0% and specificity in the presence of degradation products and excipients.

Conclusion: The results presented in this paper showed that the developed method was fast and applicable, for the separation and determination of amlodipine/benazepril combination in capsules.

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