FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE
DOI:
https://doi.org/10.22159/ijpps.2019v11i3.30594Keywords:
Litsea laevigata,, DPPH assay,, Reducing antioxidant power and antioxidant activityAbstract
Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family.
Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata.
Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts.
Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.
Downloads
References
Sies H. Oxidative stress, oxidants, and antioxidants. Exp Physiol 1997;82:291-5.
Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176-86.
Devasagayam TPA, Tilak JC, Boloor KK. Review: free radical and antioxidants in human health. Curr Stat Fut Pros JAPI 2004;53:794-804.
Perron NR, Brumaghim JL. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys 2009;53:75-100.
Gao CY, Tian CR, Zhou R, Zhang RG, Lu YH. Phenolic composition, DNA damage protective activity and hepatoprotective effect of free phenolic extract from Sphallerocarpus gracilis seeds. Int Immunopharmacol 2014;20:238-47.
Halliwell B. Biochemistry of oxidative stress. Biochem Soc Trans 2007;35:1147-50.
Harman D. Free radical theory of aging. Elsevier 1998;20:3–7.
Gulcin I. Antioxidant activity of food constituents, an overview. Arch Toxicol 2012;86:345–91.
Sireesha K, Rao SP. Oxidative stress and diabetes: an overview. Asian J Pharm Clin Res 2015;8:15-9.
Huang DJ, Chen HJ, Lin CD, Lin YH. Antioxidant and ant proliferative activities of water spinach (Ipomea aquatica Frosk.) constituents. Bot Bull Acad Sin 2005;406:99-106.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity phenols, flavanoid contents of selected Iranian medicinal plants. S. Afr J Biotechnol 2006;5:1142-5.
Duh PD, Tu YY, Yen GC. Antioxidants activity of aqueous extract of harnjyur (Chrysanthemum morifolium Ramat). Lebensmwiss Technol 1999;32:269-77.
Priyanga S, Mary MR, Hemmalakshmi S, Devaki K. Antihyperlipidemic effect of aqueous extract of Aegle marmelos and Camellia sinensis in oil-fed hyperlipidemic rats. Int J Pharm Pharm Sci 2014;3:338-41.
Makkar HPS. Quantification of tannin in tree and shrub foliage: a laboratory manual Dondrecht. The Netherlands: Kluwer Academic Publishers; 2003.
Zhishen J, Mengecheng T, An Jianming W. The determination of flavonoids contents on mulberry and their scavenging effects on superoxide radical. Food Chem 1999;64:555-9.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;29:1199-200.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.
Dinis TC, Madeira VM, Almeida LM. Action of phenolic derivatives (acetoaminophen, salycilate and 5 aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Bio Phys 1994;315:161-9.
Beauchamp C, Fridovich I. Superoxide dismutase, Improved assay applicable to acrylamide gels. Anal Biochem 1971;44:276-7.
Oyaizu M. Studies on products of browning reactions, antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr 1986;44:307–15.
Pulido R, Bravo L, Sauro-Calixo F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/ antioxidant power assay. J Agric Food Chem 2000;48:3396-402.
Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem 2007;105:950-8.
Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003‐8.
Chung KT, Wong TY, Huang YW, Lin Y. Tannins and human health: a review. Crit Rev Food Sci Nutr 1998;38:421–64.
Okudu T, Yoshida T, Hatano T. Food phytochemicals for cancer prevention II. In: Ho C, Osawa T, Huang MT, Rosen RT. Chemistry and antioxidative effects of phenolic compounds from licorice, tea, and Compositae and Labiateae herbs; Washington, DC: American Chemical Society; 1994. p. 132-43.
Tepe B, Sokmen M, Akpulat HA, Sokmen A. Screening of the antioxidant potentials of six salvia species from turkey. Food Chem 2006;95:200-4.
Crozier A, Ashihara H, Clifford MN. Plant secondary metabolites and the human diet. Blackwell Publishing. Oxford. England; 2006.
Porto CD, Calligaris S, Celloti E, Nicoli MC. Antiradical properties of commercial cognacs assessed by the DPPH test. J Agric Food Chem 2000;48:4241-5.
Loo AY, Jain K, Darah I. Antioxidant activity of compounds isolated from the pyroligneous acid, Rhizophora apiculata. Food Chem 2008;107:1151–60.
Soler Rivas C, Espin JC, Wichers HJ. An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Phytochem Anal 2000;11:330–8.
Pazos M, Gallardo JM, Torres JL, Medina I. Activity of grape polyphenols as inhibitors of the oxidation of fish lipids and fish muscle. Food Chem 2005;92:547–57.
Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. Afr J Microbiol Res 2009;13:981‐96.
Nishikimi M, Rao NA, Yagi K. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 1972;46:849-54.
Pellegrini N, Serafini M, Colombi B, Rio DD, Salvatore S, Bianchi M, et al. Total antioxidant capacity of plant foods, beverages and oils consumed in italy assessed by three different in vitro assays. J Nutr 2003;133:2812–9.
Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW, et al. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agric Food Chem 1998;46:1887–92.
Wang H, Gao XD, Zhou GC, Cai L, Yao WB. In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chem 2008;106:888–95.
Halvorsen BL, Carlsen MH, Phillips KM, Bohn SK, Holte K, Jacobs DR, et al. Content of redox-active compounds (i.e., antioxidants) in foods consumed in the United States. Am J Clin Nutr 2006;84:95–135.
Published
How to Cite
Issue
Section
