THE INFLUENCE OF SODIUM ORTHOVANADATE ON P85 AND GSK-3 EXPRESSIONS TO THE BLOOD GLUCOSE REGULATION OF TYPE 2 DIABETIC MICE (MUS MUSCULUS) MODEL

Authors

  • Rima H. Ritonga Airlangga University
  • Budi Suprapti Airlangga University
  • Junaidi Khotib Airlangga University

Keywords:

Sodium orthovanadate, Diabetes mellitus, P85 (Regulatory Subunit of PI3-Kinase), GSK-3 (Glycogen Synthase Kinase 3)

Abstract

Objective: The present study was designed to investigate the influence of sodium orthovanadate on the P85 (Regulatory Subunit of PI3-Kinase) and GSK-3 (Glycogen Synthase Kinase 3) expressions in skeletal muscle tissue to the decreasing of blood glucose levels of alloxan-induced diabetic mice.

Methods: Mice were divided into 5 groups i. e. (1) naive group, (2) diabetic group and (3-5) orthovanadate-treated diabetic groups at the dose of 16, 32 and 64 mg/kg BW respectively. Diabetic mice model was induced by intraperitoneal administration of alloxan monohydrate at the dose of 200 mg/kgBW. Diabetic state was occurred on day 3 after alloxan injection and then started the treatment of sodium orthovanadate for 7 days. The muscular tissue was harvested on day 10 after treatment and was stained using routine histology staining, haematoxylin-eosin for morphological qualitative analysis and immunohistochemical approaches to observe the expressions of P85 and GSK-3 in skeletal muscle.

Results: Diabetes condition was shown by the increasing of fasting blood glucose levels from 59.1 ± 11.2 mg/dL to 310.6 ± 107.2 mg/dL on day 3. Administration of sodium orthovanadate at the dose of 16, 32 and 64 mg/kg BW reduced the fasting blood glucose levels after 7 days treatment (p < 0.001) at diabetic mice significantly. The results of histology staining showed that sodium orthovanadate improved a necrosis in skeletal muscle cells alloxan-induced diabetic mice. On immunohistochemical approaches, sodium orthovanadate might decreased the P85 expressions and increased the GSK-3 expressions in skeletal muscle cells alloxan-induced diabetic mice (p < 0.001).

Conclusion: This study reveals that the administration of sodium orthovanadate on diabetic mice led to the attenuation of the PTPase activity. The inhibition cause a decreasing the expression of P85 and increasing of GSK-3 that cause the decreasing of blood glucose levels and an improvement of insulin target cells in the muscle cells.

 

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Author Biographies

Rima H. Ritonga, Airlangga University

Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia

Budi Suprapti, Airlangga University

Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia

Junaidi Khotib, Airlangga University

Department of Clinical Pharmacy, Faculty of Pharmacy, Airlangga University, Surabaya, Indonesia

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Published

01-01-2015

How to Cite

Ritonga, R. H., B. Suprapti, and J. Khotib. “THE INFLUENCE OF SODIUM ORTHOVANADATE ON P85 AND GSK-3 EXPRESSIONS TO THE BLOOD GLUCOSE REGULATION OF TYPE 2 DIABETIC MICE (MUS MUSCULUS) MODEL”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 1, Jan. 2015, pp. 115-9, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/3157.

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Original Article(s)