PHYTOCHEMICAL INVESTIGATIONS AND ANTIMICROBIAL AND ANTICANCER ACTIVITIES OF HOMONOIA RIPARIA LOUR
Keywords:
Homonoia riparia, Escherichia coli, Proteus vulgaris, Staphylococcus aureus, Bacillus cereus, Trichophyton mentagrophytes, Trichophyton rubrum, antimicrobial activityAbstract
Objective: Present study aims at the evaluation of antibacterial activity against Escherichia coli, Proteus vulgaris, Staphylococcus aureus and Bacillus cereus, antifungal activity against Trichophyton mentagrophytes and T. rubrum and anticancer activity against human lung cancer cell line - A549 of extracts of Homonoia riparia Lour. The extracts showing antimicrobial activity were further subjected to phytochemical analysis to determine the major group of compounds present.
Methods: The procedures used for testing the antimicrobial and anticancer activity were as per the prescribed methods.
Results: DMSO extracts of male flowers inhibited growth of P. vulgaris and S. aureus whereas, aqueous extracts of both leaves and male flowers inhibited growth of T. mentagrophytes. Phytochemical analysis of these extracts showed the presence of tannins, saponins and flavonoids in leaves and flowers. Tannins, saponins and flavonoids were separated by traditional extractive methods. MIC of tannins from flowers was 50 mg ml-1against P. vulgaris and of tannins and flavonoids was 40 mg ml-1against S. aureus. The extracted tannins, flavonoids, saponins and steroids and triterpenoids from leaves and flowers inhibited the growth of T. mentagrophytes. TLC of these compounds showed presence of a few common components in the leaves and flowers.
The ethanolic extracts of H. riparia did not exhibit anticancer activity.
Conclusion: The leaves and male flowers of H. riparia possess antimicrobial activity. The tannins, flavonoids, saponins, steroids and triterpenoids are present in leaves and flowers and inhibit the growth of P. vulgaris, S. aureus and T. mentagrophytes.
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Assob JC, Kamga HL, Nsagha DS, Njunda AL, Nde PF, Asongalem EA, et al. Antimicrobial and toxicological activities of five medicinal plant species from cameroon traditional medicine. BMC Complementary Altern Med 2011;11(70):1-11.
National Institute of Science Communication, CSIR (IN). The Wealth of India-A Dictionary of Indian Raw Materials and Industrial Products, Raw Materials Vol. V: H-K. New Delhi: CSIR; 1959.
Viswanadh GS, Ramaiah PA, Laatsch H, Maskey R. Chemical constituents of the heartwood and bark of Homonoia riparia. J Trop Med Plants 2006;7(2):267-73.
Shu-min Y, Xi-kui L, Chen Q, Da-gang W, Da-yuan Z. Chemical constituents from the roots of Homonoia riparia. Acta Pharm Sin 2007;42(3):292-6.
Auwal MS, Fatima AL, Shuaibu A, Ismaila AM, Kyari AS, Thaluvwa AB, et al. Phytochemical properties and effect of aqueous extract of Jatropha caucus root bark on some bacterial isolates. J Pharmacogn Phytother 2013;5(8):148-52.
Aneja KR. Experiments in Microbiology, Plant Pathology and Tissue Culture. New Delhi: Wishwa Prakashan, A division of Wiley Eastern Limited; 1993.
Hamid AA, Aiyelaagbe OO, Ahmed RN, Usman LA, Adebayo SA. Preliminary phytochemistry, antibacterial and antifungal properties of extracts of Asystasia gangetica Linn T. Anderson grown in Nigeria. Adv Appl Sci Res 2011;2(3):219-26.
Beltagy AM. Investigation of new antimicrobial and antioxidant activities of Brassica rapa. Int J Pharm Pharm Sci 2014;6(6):84-8.
Abubacker MN, Ramanathan R, Senthil Kumar T. In vitro antifungal activity of Cassia alata Linn. flower extract. Nat Prod Rad 2008;7(1):6-9.
Webster D, Taschereau P, Rene BJ, Sand C, Rennie RP. Antifungal activity of medicinal plant extracts; preliminary screening studies. J Ethnopharmacol 2008;5(1):140-6.
Nelson MM, Martin AG, Heffernan MP. Superficial fungal infections: Dermatophytosis, Onychomycosis, Tinea nigra and Piedra. In: Freedberg IM, Eisen AZ, Wolff K, Austen KF, Goldsmith LA, Katz SI, editors. Fitzpatrick’s Dermatology in General Medicine, Vol. II. 6th ed. United States of America: Mcgraw-Hill, Medical Publishing Division; 2003. p. 1989-92.
Larone DH. Medically important fungi-a guide to identification. Maryland: Harper and Row Publishers; 1976.
Gaidhani SN, GS Lavekar GS, Juvekar AS, Sen S, Singh A, Kumari S. In-vitro anticancer activity of standard extracts used in ayurveda. Pharmacognosy Magazine 2009;5(20):425-9.
Harborne JB. Phytochemical Methods-A Guide to modern techniques of plant analysis. 3rd ed. New Delhi: Springer (India) Private Limited; 1998.
Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 39th ed. Pune: Nirali Prakashan; 2007.
Sadasivam S, Manickam A. Biochemical methods. 3rd ed. New Delhi: New Age International (P) Ltd; 2008.
Brindha S, Thamaraiselvi T, Maragathavalli S, Annadurai B, Gangwar SK. Studies on the chemical and medicinal value of Vitex negundo Linn. Int J Adv Biol Res 2012;2(2):298-301.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 2005;4(7):685-8.
Malu SP, Obochi GO, Edem CA, Nyong BE. Effect of methods of extraction on phytochemical constituents and antibacterial properties of Tetrecarpidium conophorum seeds. Global J Pure Appl Sci 2009;15(3):373-6.
Bai X, Qiu A, Guan J. Optimization of microwave-assisted extraction of antihepatotoxic triterpenoid from Actinidia deliciosa root and its comparison with conventional extraction methods. Food Technol Biotechnol 2007;45(2):174-80.
Auribie MA. Antioxidant activity of tannin from Tamarix aphylla L. leaves. Basrah J Agric Sci 2011;24(1):406-10.
Wagner H, Bladt S, Zgainski EM. Plant drug analysis-a thin layer chromatography atlas. Verlag: Springer; 1984.
Wagner H, Bladt S. Plant drug analysis-A thin layer chromatography atlas. 2nd ed. Germany: Springer; 1996.
Dinan L, Hramatha J, Lafont R. TLC of sterols, steroids and related triterpenoids. In: Waksmundzka-Hajnos M, Sherma J, Kowalska T, editors. Thin layer chromatography in phytochemistry. Boca Raton: CRC Press, Taylor and Francis Group; 2008. p. 581.
Sherma J. Steroids. In: Sherma J, Fried B, editors. Handbook of thin layer chromatography. 3rd ed. New York: Marvel Dekker; 2003. p. 919.
Schmitz D, Zapp J, Bernhardt R. Hydroxylation of the triterpenoid dipterocarpol with CYP106A2 from Bacillus megaterium. Federation Eur Biochem Soc J 2012;279:1663–74.
Patil RS, Godghate AG, Sawant RS. Phytochemicals and antimicrobial activity of leaves of Homonoia riparia. Int J Pharm Bio Sci 2014;5(2):352-6.
Spjut RW. Relationships between plant folklore and antitumour activity: an historical review. SIDA 2005;21(4):2205-41.
Parveen N, Singh MP, Khan NU. Chemical examination of the leaves of Homonoia riparia Lour. (Euphorbiaceae). J Indian Chem Soc 1988;65(11):815-6.