GC-MS ANALYSIS, HPTLC FINGERPRINT PROFILE AND DPPH FREE RADICAL SCAVENGING ASSAY OF METHANOL EXTRACT OF MARTYNIA ANNUA LINN SEEDS
DOI:
https://doi.org/10.22159/ijpps.2019v11i6.32206Keywords:
Martynia annua, GC-MS, HPTLC fingerprint, Antioxidant activityAbstract
Objective: To investigate the photochemical constituents present in methanol extract of martynia annua seeds using Gas Chromatography-Mass Spectroscopy(GC-MS), High-Performance Thin–Layer Chromatography(HPTLC) analysis and study antioxidant activity.
Methods: Methanol extract of Martynia annua seeds were subjected to GC-MS and HPTLC analysis. HPTLC analysis was carried out using GAMAG system with a linomate5 applicator, system mobile phase (Toluene: Chloroform: Ethanol (4:4:1 V/V/V)), two different volume of extract was applied 2 µl and 5 µl. GC-MS analysis was carried out on JEOL GC MATE ΙΙ, column HP 5 MS and Quadruple double focusing mass analyzer. Antioxidant activity was determined by DPPH assay.
Results: GC-MS analysis provided 17 peaks indicating the presence of seventeen different phytochemicals in methanol extract of martynia annua seeds. HPTLC fingerprint showed 6 peaks at both size 2 µl and 5 µl at 254 nm whereas 4 peaks, 9 peaks were detected at 366 nm for 2 µl and 5 µl respectively. After derivatization with 10 % methanolic sulphuric acid, 8 peaks, 11 peaks were detected for 2 µl and 5 µl respectively when the derivatized plate was scanned at 540 nm. DPPH free radical scavenging result showed EC50 value of 44.1±1.1 µg/ml.
Conclusion: The GC-MS analysis showed the presence of fatty acids, ester, aldehydes and ketones whereas in HPTLC different peaks at different UV-lights before and after derivatization were observed. Maximum percentage inhibition using DPPH assay was found 74 at concentration of 50 µg/ml.
Downloads
References
Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health perspect 2001;109 Suppl 1:69-75.
Revishankar B, Shukla VJ. Indian system of medicine: a brief profile. Afr J Tradit Complement Altern Med 2007;4:319-37.
Harish BB, Mohana LS, Saravana KA. Studies on phytochemical and anticonvulsant property of Martynia annua linn. Int J Phytopharmacol 2010;1:82-6.
Zarina A, Sumayya S, Shaista N. Antioxidant activities and phytochemical screening of Martynia annua seeds extract. Biosci Biotech Res Asia 2017;14:1363-9.
Prakash P, Prabhakar S, Ramchndra G, Ashish G, Ajag S, Nitin N, et al. Formulation and evaluation of herbal effervescent granules incorporated with martynia annua extract. J Drug Discovery Ther 2013;1:54-7.
Eswaraiah MC, Eswarudu MM, Kumar KP, Kumar PS, Kumar TT. An overview on Martynia Annua L. Int J Pharm Res Dev 2013;5:113-8.
Ashwani KD, Bhawna C, Sanjeev KM. Martynia annua L.: a review on its ethnobotany, phytochemical and pharmacological profile. J Pharmacogn Phytochem 2013;1:135-40.
Renato B, Luciana GW, Gisely CL, Joao CPD. Quality control and TLC profile data on selected plants species commonly found in the Brazilian market. Rev Bras Farmacogn Braz J Pharmacogn 2012;22:1111-8.
Elangovan NM, Dhanarajan MS, Elangovan l. Preliminary phytochemical screening and HPTLC fingerprinting profile of leaf extracts of Moringa oleifera and Phyllanthus emblica. Int Res J Pharm Biosci 2015;2:32-40.
Shakila R, Ganesan R, Arul AS, Duraipandiyan V. Analytical and HPTLC studies on Coldenia Procumbens linn. whole plant. J Innovations Pharm Biol Sci 2017;4:28-35.
Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Brazillian J Med Biol Res 2000;33:179-89.
Gayathri G, Saraswathy A, Vijayalakshmi K. HPTLC fingerprint profile of Bauhinia variegate Linn. leaves. Asian Pacific J Trop Dis 2012;2 Suppl 1:521-5.
Murugesan S, Bhuvaneswari S. HPTLC fingerprint profile of methanol extract of the marine red alga portieria hornemannii (Lyngbye) (Silva). Int J Adv Pharma 2016;5:61-5.
Shulammithi R, Sharanya M, Tejaswini R, Kiranmai M. Standardization and quality evaluation of herbal drugs. IOSR J Pharm Biol Sci 2016;11:89-100.
Anjoo K, Ajay KS. HPTLC fingerprint profile of extracts from dried aerial parts of Bryophyllum pinnatum in different solvents. Pharmacogn J 2010;2:24-31.
Pravin HN, Joseph K, Aruna J, Vilasrao K. Future trends in the standardization of herbal drugs. J Appl Pharm Sci 2012;2:38-44.
Reich E, Schibli A. High-performance thin layer chromatography for the analysis of medicinal plants. 1st ed. Thieme medical publisher, Inc. The Americans, Ny; 2007.
Sreejayan N, Rao MN. Free radical scavenging activity by curcuminoids. Arzneimittelforschung 1996;46:169-71.
Chandana NDML, Jaya PRP, Kondammagari S, Borelli DPR, Nannepaga JS. Phytoconstituents profile of clitoria ternatea by GC-MS and its age-related anticholinergic activity against aluminum and restraint stress. Int Res J Pharm 2018;9:38-44.
Mustapha NA, Runner RTM. GC-MS analysis and preliminary antimicrobial activity of Albizia adianthifolia (Schumach) and Pterocarpus angolensis (DC). Medicines 2016;3:1-9.
Brinda C, Mohan KR. Evaluation of the antioxidant,antibacterial activity of ethanolic extract in the leaves of Combretum albidum and gas chromatography-mass spectrometry analysis. Asian J Pharm Clin Res 2016;9:325-9.
Rajendran P, Bharathidasan R, Sureshkumar K. GC-MS analysis of phyto-components in raw and treated sugarcane juice. Int J Curr Microbiol Appl Sci 2017;6:51-61.
Sheeba GD, Viswanathan P. GC-MC analysis of phytocomponents in Spermacoce articularis L. f. leaf. Res Pharm 2014;4:1-7.
Nisha, Pasaumarti BR. Gas chromatography-mass spectrometry analysis for identification of bioactive compounds in selected genotypes of Trigonella foenum-graecum L. Pharma Innovation 2018;7:929-39.
Padmashree MS, Roopa B, Ashwathanarayana R, Raja N. Antibacterial properties of Ipomoea staphylina roem and schult. Plant extracts with comparing its preliminary qualitative phytochemical and quantitative GC-MS analysis. Trop Plant Res 2018;5:349-69.
Ali A, Javaid A, Shoaib A. GC-MS analysis and antifungal activity of methanolic root extract of Chenopodium album against Scletotium rolfsii. Planta Daninha 2017;35:1-8.
Karuppasamy R, Veerabahu RM. GC-MS analysis of bioactive components of Myxopyrum serratulum A. W. hill (Oleacea). Int J Pharm Sci Rev Res 2016;38:30-5.
Vardhini SP, Sivaraj C, Himanshu R, Arumugam P. GC-MS, photochemical analysis and antioxidant activities of fruit pulp of Aegle marmelos (L.) correa. Eur J Biomed Pharm Sci 2018;5:569-74.
Konovalova O, Gergel E, Herhel V. GC-MS analysis of bioactive components of Shepherdia argentea (Pursh.) nutt. From Ukrainian. Pharma Innovation J 2013;2:7-12.
Siti K, Grace N, Irda F. Antioxidant activities from various extracts of different parts of Kelakai (Stenochlaena palustaris) grown in central Kalimantan-Indonesia. Asian J Pharm Clin Res 2016;9 Suppl 1:215-9.
Published
How to Cite
Issue
Section
