EFFECT OF GAMMA IRRADIATION ON ANTIOXIDANT POTENTIAL AND BIOACTIVES OF A COSMECEUTICALLY SIGNIFICANT CHLORELLA EMERSONII KJ725233: ALTERATIONS IN CEK AS A RESPONSE TO GAMMA IRRADIATION

Sneha Sunil Sawant; Reema Devi Singh; Sukhendu Ghosh; Varsha Kelkar Mane

doi:10.22159/ijpps.2019v11i8.33153

Authors

Sneha Sunil Sawant Department of Biotechnology, University of Mumbai, Kalina, Santacruz (E), Mumbai, Maharashtra, India 400098
Reema Devi Singh Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center, Trombay, Mankhurd, Mumbai, Maharashtra, India 400085
Sukhendu Ghosh Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Center, Trombay, Mankhurd, Mumbai, Maharashtra, India 400085
Varsha Kelkar Mane Department of Biotechnology, University of Mumbai, Kalina, Santacruz (E), Mumbai, Maharashtra, India 400098

DOI:

https://doi.org/10.22159/ijpps.2019v11i8.33153

Keywords:

Gamma irradiation, C emersonii KJ725233, Antioxidant, Vitamin E, Cosmeceutical

Abstract

Objective : Gamma radiation induces free radicals with a corresponding alteration in the cell’s antioxidant defense system. The present study thus aimed at determining the role of gamma irradiation in improving the cosmeceutical potential of CEK in terms of antioxidants.

Methods : C.emersonii KJ725233 was subjected to low (100 Gy) and high (1000 Gy) gamma irradiation doses and its effect on the chlorophyll content was evaluated. The quantitative alterations in the antioxidant content of CEK were evaluated by phosphomolybdenum assay (TAC), ferric reducing antioxidant potential (FRAP), 2, 2 - Dipheny-1-picryl hydrazyl radical scavenging assay (DPPH), total phenolic (TPC) and total flavonoid content (TFC). Also, the corresponding qualitative alterations in the bioactives of CEK were determined by GC-HRMS analysis.

Results : A 179.57±2.55% increase in the total chlorophyll content alongwith a 71.76±2.96%, 32.08±2.16%, 11.67±0.89%, 42.85±8.0% and 31.37±3.18% increase was observed in the TAC, FRAP, DPPH radical scavenging, TPC and TFC was observed in CEK irradiated at 1000 Gy. GC-HRMS analysis revealed the induction of Vitamin E on irradiation at both the doses with a corresponding decrease in the phytol content whereas 100 Gy stimulated the induction of phytosterols.

Conclusion : The potent intrinsic antioxidant activity of cosmeceutically significant CEK can be elevated with the induction of the most sought after antioxidant in cosmetology – Vitamin E on irradiation.

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