A PRELIMINARY INVESTIGATION OF HTR1A GENE EXPRESSION LEVELS IN AUTISM SPECTRUM DISORDERS
DOI:
https://doi.org/10.22159/ijpps.2019v11i8.34141Keywords:
Autism spectrum disorders, Gene expressions, HTR1A gene expressionAbstract
Objective: This study was conducted to explore the expression levels of HTR1A gene in a sample of Egyptian autistic children.
Methods: Thirty autistic patients (18 boys, 12 girls) and 20 controls were enrolled in the study. From each child, we isolated RNA samples from whole blood. Quantitative Real-Time PCR (qRT-PCR) was used to measure the gene expressions of HTR1A and normalized to the house keeping gene, beta-actin.
Results: The HTR1A gene expression of healthy controls and ASD subjects were varied significantly (p =0.0062). As compared to control healthy subjects, the HTR1A expressions were greatly reduced in samples of ASD.
Conclusion: HTR1A gene expression level is a candidate gene for further studies to explore its potential roles in ASD related pathways.
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