Antitumor Effects and Characterization of Biosynthesized Iron oxide nanoparticles using seaweeds of Gulf of MannarANTITUMOR EFFECTS AND CHARACTERIZATION OF BIOSYNTHESIZED IRON OXIDE NANOPARTICLES USING SEAWEEDS OF GULF OF MANNAR

Authors

  • Sangeetha N. madurai kamaraj university
  • A. K. Kumaraguru Madurai Kamaraj University

Keywords:

Antitumor effect, Cancer, Flutamide, Gulf of Mannar, Iron oxide nanoparticles, Seaweeds

Abstract

Objective: Three different seaweeds were used for biosynthesis of iron oxide nanoparticles. The screening of seaweeds, and suitable conditions for iron oxide nanoparticle synthesis were analyzed using different optimizing parameters such as concentration of seaweed extract, reaction temperature, and pH. It was found that only one seaweed, Sargassum myriocystem was able to synthesized iron oxide nanoparticles.

Methods: Biosynthesized iron oxide particles were characterized and confirmed through the techniques such as X-ray diffraction, Atomic force microscopy, Scanning electron microscope, Transmission electron microscopy and Fourier transform spectroscopy.

Results: Transmission electron microscopy showed image size of iron oxide nanoparticles as 6 - 12 nm. This biosynthesized S. myriocystum leaf extract biomolecules act as precipitating and capping agent. Formulation of biosynthesized iron oxide nanoparticle with cancer drug flutamide showed in-vitro drug release of 91% which was confirmed with standard flutamide curve. Drug carrier effect in tumors cell through in vivo assay was done to analyze life span of mice, liver enzymes and hematological parameters,. Overall results of In-vivo assay indicated that biosynthesized iron oxide nanoparticles act as good tumor targeted drug carrier with antitumor effect.

Conclusion: As evident from the present study, S. myriocystum leaf extract can be used for synthesis iron oxide nanoparticles of 2.8 nm. Biosynthesized iron oxide being smaller and narrow in size which has been used for various types of cancer treatment they were selected further for in-vitro and in-vivo analysis.

 

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References

Kato H. In vitro assays: tracking nanoparticles inside cells. Nat Nanotechnol 2011;6(3):139-40.

Rishton A, Lu Y, Altman RA, Marley AC, Bian Hahnes C, Viswanathan R, et al. Magnetic tunnel junctions fabricated at tenth-micron dimensions by electron beam lithography. Microelectron Eng 1997;35:249-52.

Osaka T, Matsunaga T, Nakanishi T, Arkaki A, Niwa D, Iida H. Synthesis of magnetic nanoparticles and their application to bioassays. Anal Bioanal Chem 2006;384:593-600.

Yang Hung-Wei, Hua Mu-Yi, Liu Hao-Li, Huang Chiung-Yin, Wei Kuo-Chen. Potential of magnetic nanoparticles for targeted drug delivery. Nanotechnol Sci Appl 2012;5:73-86.

Sweetman SC. (ed.) Martindale, Complete Drug Reference. 34th ed. Pharmaceutical Press, London; 2005. p. 556-7.

Peng S, Wang C, Xie J, Sun S. Synthesis and stabilization of monodisperse Fe nanoparticles. J Am Chem Soc 2006;128:10676-7.

Ganesapandian S, Kumaraguru AK. Seaweeds resourse in the intertidal and subtidal regions of pudhumadam Gulf of mannar. Seaweed Res Utilization 2008;30:97-105.

Ramachandra Reddy G, Mahaveer S Bhojani, Patrick McConville, Jonathan Moody, Bradford A. Vascular targeted nanoparticles for imaging and treatment of brain tumors. Clin Cancer Res 2006;12:6677-86.

Jacqueline MH, Darius JN, Mathew JM, Ronald DB. Blood lipid profile in children’s with acute lymphoblastic leukemia. Cancer 1998;83:379-84.

Jahan AK, Sulthan S, Rowshanul H, Kumar S, Mosin A. Antineoplastic activity of Bis–Tyrosinediaqu Ni (II) against ehrlich ascites carcinoma. J Pharm Sci 2008;7:33-7.

Price VE, Greenfield RE. Anemia in cancer. Adv Cancer Res 1958;5:199-200.

Hogland HC. Hematological complication of cancer chemotherapy. Semin Oncol 1982;9:95-102.

Viroj wiwanikit. High serum alkaline phosphatase level in hospitalized patient. BMC Family Practice 2001;10(1861):1471-2296.

Shen L, Laibinis PE, Hatton TA. Bilayer surfactant stabilized magenetic fluids synthesis and interactions at interfaces. Langmuir 1999;15:447-53.

Gupta AK, Gupta M. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 2005;26:3995-4021.

Laurent S, Forge D, Port M, Roch A, Robic C, Vander Elst L, et al. Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications. Chem Rev 2008;108(6):2064-110.

Ziolo RF, Giannelis EP, Weinstein BA, Ohoro MP, Ganguly BN, Mehrotra V, et al. Matrix-mediated synthesis of nanocrystalline ggr-Fe2O3: A New Optically Transparent Magnetic Material 1992;10(257):219-23.

Igartua M, Saulnier P, Heurtault B, Pech B, Proust JE, Pedraz JL, et al. Development and characterization of solid lipid nanoparticles loaded with magnetite. Int J Pharm 2002;233:149-57.

Nidhin M, Indumathy R, Sreeram KJ, Balachandran Unni Nair. Synthesis of iron oxide nanoparticles of narrow size distribution on polysaccharide templates Bull. Mater Sci 2008;31(1):93-6.

Kim DK, Y Zhang, W Voit, KV Rao, J Kehr, B Bjelke, et al. Superparamagnetic iron oxide nanoparticles for bio-medical applications. Scripta Mater 2001;44:1713-7.

Inouye K, Endo R, Otsuka Y, Miyashiro K, Kaneko KT. Ishikawa micelle and reversed microemultion. J Phys Chem 1982;86:1465-9.

Canizal G, Ascencio JA, Gardea-Torresday J, Jose-Yacaman M. Multiple twinned gold nanorods grown by bio-reduction techniques. J Nanoparticle Res 2001;3:475-81.

Rautaray D, Ahmad A, Sastry M. Biosynthesis of CaCO3 crystals of complex morphology using a fungus and an actinomycete. J Am Chem Soc 2003;125(48):146-56.

Basavaraja S, Balaji SD, Lagashetty A, Rajasab AH, Venkataraman. A Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium semitectum. Mater Res Bull 2008;43(5):1164-70.

Donini C, Robinson DN, Colombo P, Giordano F, Papas NA. Preparation of poly(methacrylic acid-g-poly(ethylene glycol)) nanospheres from methacrylic monomers for pharmaceutical applications. Int J Pharm 2002;245:83-91.

Emad al din Haratifar, Hamid Reza Shahverdi, Mojtaba Shakibaie, Kamyar Mollazadeh Moghaddam, Mohsen Amini, Hojatollah Montazeri, et al. Semi-Biosynthesis of magnetite-gold composite nanoparticles using an ethanol extract of eucalyptus camaldulensis and study of the surface chemistry. J Nanomater 20091-5:5.

Sipos P, Berkesi O, Tombacz E, St Pierre TG, Webb J. Formation of spherical iron (III) oxyhydroxide nanoparticles sterically stabilized by chitosan in aqueous solutions. J Inorg Biochem 2003;95:55-63.

Hui-li Maa, Xian-rong Qi, Yoshie Maitani suneji Nagai T. Preparation and characterization of superparamagnetic iron oxide nanoparticles stabilized by alginate. Int J Pharm 2007;333:177-86.

Davis TA, Volesky B, Mucci A. A review of the biochemistry of heavy metal biosorption by brown algae. Water Res 2003;37:4311-30.

Shenoy D, Little S, Langer R, Amiji M. Poly(ethylene oxide)-modified poly(β-amino ester) nanoparticles as a pH-sensitive system for tumor-targeted delivery of hydrophobic drugs: in-vitro evaluations. Mol Pharm 2005;2(5):357-66.

Clarkson BD, Burchenal JH. Preliminary screening of antineoplastic drugs. Prog Clin Cancer 1965;1:625-9.

Arora S, Jain J, Rajwade JM, Paknikar KM. Cellular responses induced by silver nanoparticles: In vitro studies. Toxicol Lett 2008;179:93-100.

Adlin, Jino Nesalin, Gowthamarajan K, Somashekhara CN. Formulation and evaluation of nanoparticles containing flutamide. Inter J Chem Tech Res 2009;1(4):1331-4.

Gupta M, Mazumder U, Sambath Kumar R, Siva Kumar T. Antitumor activity and antioxident role of Bauhinia racemosa against Ehrlich ascites carcinoma in Swiss albino mice. Acta Pharmacol Sin 2004;25(8):1070-6.

Prasad SB, Giri A. Anti tumor effect of cisplatin against murine ascites dalton’s lymphoma. Indian J Exp Biol 1994;32:155-62.

Fenninger LD, Mider GB. Energy and nitrogen metabolism in cancer. Adv Cancer Res 1954;2,229-53.

De Wys, William D. Pathophysiology of tumour cachexia: current understanding and areas for future research. Tumour Res 1982;42:721-5.

Published

01-02-2015

How to Cite

N., S., and A. K. Kumaraguru. “Antitumor Effects and Characterization of Biosynthesized Iron Oxide Nanoparticles Using Seaweeds of Gulf of MannarANTITUMOR EFFECTS AND CHARACTERIZATION OF BIOSYNTHESIZED IRON OXIDE NANOPARTICLES USING SEAWEEDS OF GULF OF MANNAR”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 2, Feb. 2015, pp. 469-76, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/3755.

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