HEPATOPROTECTIVE ACTIVITY OF MERREMIA BORNEENSIS AGAINST CARBON TETRACHLORIDE (CCl4)-INDUCED ACUTE LIVER DAMAGE IN RATS: A BIOCHEMICAL AND HISTOPATHOLOGICAL EVALUATION

MOHAMMAD IQBAL; MUHAMMAD DAWOOD SHAH; SENTY VUN-SANG; RIANA BINTI AWANG SAMAN

doi:10.22159/ijpps.2020v12i10.38905

Authors

MOHAMMAD IQBAL Biotechnology Research Institute
MUHAMMAD DAWOOD SHAH Biotechnology Research Institute, Borneo Marine Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia
SENTY VUN-SANG Biotechnology Research Institute
RIANA BINTI AWANG SAMAN Biotechnology Research Institute

DOI:

https://doi.org/10.22159/ijpps.2020v12i10.38905

Keywords:

Merremia borneensis, Oxidative stress, Carbon tetrachloride, Hepatoprotective activity, Histopathology

Abstract

Objective: The pathogenesis of various liver injuries involves oxidative damage. This research was planned to examine the effects of Mereemia borneensis extract on hepatic oxidative damage caused by carbon tetrachloride (CCl₄) in rats.

Methods: Sprague Dawley rats were exposed to M. borneensis (125 and 250 mg/kg b. wt.) once daily for 14 d followed by two doses of CCl₄ (1.2 ml/kg b. wt.). After 2 w, the rats were sacrificed and hepatoprotective analysis was done.

Results: Orally administration of CCl₄ enhances serum transaminase (ALT; alanine transaminase and AST; aspartate transaminase), γ-glutamyl transpeptidase, lipid peroxidation, reduction in glutathione, catalase, glutathione reductase, glutathione peroxidase, quinone reductase and glutathione S-transferase. Pretreatment of rats with M. borneensis at 125 and 250 mg/kg body weight significantly reduced levels of ALT, AST, γ-glutamyl transpeptidase and lipid peroxidation of CCl₄ treated rats. Pretreatment with M. borneensis against rats treated with CCl₄, hepatic enzymatic and non-enzymatic antioxidant molecules have increased significantly. A decreased histopathological change in the liver is further evidence of the protective effect of M. borneensis.

Conclusion: Our data suggest that M. borneensis can be a potential hepatoprotective agent in preventing or treating degenerative diseases that involve oxidative stress.

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