ANTIMICROBIAL PROPERTIES OF SEVEN BROWN ALGAE HARVESTED FROM THE COAST OF SIDI BOUZID (EL JADIDA-MOROCCO)

NAZHA SAMRI; LAILA HSAINE; SOUKAINA ELKAFHI; SAMIRA KHLIFI; SAMIRA ETAHIRI

doi:10.22159/ijpps.2020v12i12.39362

Authors

NAZHA SAMRI Marine Biotechnology and the Environment Laboratory (BIOMARE) Faculty of Science, University Chouaib Doukkali-El Jadida-Morocco
LAILA HSAINE Marine Biotechnology and the Environment Laboratory (BIOMARE) Faculty of Science, University Chouaib Doukkali-El Jadida-Morocco
SOUKAINA ELKAFHI Marine Biotechnology and the Environment Laboratory (BIOMARE) Faculty of Science, University Chouaib Doukkali-El Jadida-Morocco
SAMIRA KHLIFI Marine Biotechnology and the Environment Laboratory (BIOMARE) Faculty of Science, University Chouaib Doukkali-El Jadida-Morocco
SAMIRA ETAHIRI Marine Biotechnology and the Environment Laboratory (BIOMARE) Faculty of Science, University Chouaib Doukkali-El Jadida-Morocco

DOI:

https://doi.org/10.22159/ijpps.2020v12i12.39362

Keywords:

Brown algae, Antibacterial activity, Antifungal activity, Solvent extracts

Abstract

Objective: This work aims at the screening of the antimicrobial activity of the seven brown marine algae of theCoast of Sidi Bouzid (El Jadida-Morocco).

Methods: The aim of this study was to evaluate the antimicrobial activity of seven brown marine algae against three Gram-positive bacteria (Staphylococcus epidermidis, Staphylococcus aureus and Streptococcus pyogenes). Three Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia) and two fungi (Aspergillus Niger and Candida tropicalis). Thus, 35 algal extracts were prepared with five organic solvents methanol/water, methanol, dichloromethane/methanol, dichloromethane and ethyl acetate. The antibacterial activity was evaluated through the disk diffusion method.

Results: Data revealed that the Staphylococcus aureus bacteria was the most sensitive pathogen by showing the highest zone of inhibitions of 20 mm with the lowest Minimum Inhibitory Concentration (MIC) of 2 μgmL⁻¹ methanol/water extract of Cystoseira tamariscifolia. Whereas, antifungal activity, the highest zone of inhibitions of 21 mm and 22 mm with the lowest Minimum Inhibitory Concentration (MIC) of 5 μgmL^-¹ was respectively shown in the methanol/water extract of Laminaria ochroleuca against Candida tropicalis and in the dichloromethanolic extract of Sargassum vulgare against Aspergillus niger.

Conclusion: The results indicate that these algal extracts can further be analyzed and purified for relevant antibacterial and antifungal compounds which can be used in therapeutics and other applications.

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References

Yong Xin L, Isuru W, Yong L, Se Kwon K. Phlorotannins as bioactive agents from brown algae. Process Biochem 2011;46:2219-24.

Kausalya GM, Narasimha Rao GM. Antimicrobial activity of marine algae. J Algal Biomass Util 2015;6:78-87.

Saleh B, Al-Mariri A. Antimicrobial activity of the marine algal extracts against selected pathogens. J Agric Sci Tech 2017;19:1067-77.

Boujaber N, Oumaskour K, Etahiri S, Assobhei O. Cytotoxic activity of some marine algae collected from the coast of sidi bouzid (El Jadida-Morocco). Int J Adv Pharm Res 2013;4:2542-7.

Mayer U, Nischt R, Poschl E, Mann K, Fukuda K, Geri M, et al. A single EGF-like motif of laminin is responsible for high affinity nidogen binding. EMBO J 1993;12:1879-85.

Bultel Ponce V, Etahiri S, Guyot M. New ketosteroids from the red alga Hypnea musciformis. Bioorg Med Chem Lett 2002;12:1715-8.

Farid Y, Chennaoui M, Assobhei O, Etahiri S. Screening des algues marines d’Oualidia a la recherche d’activités antimicrobiennes et anti-inflammatoire. Rev Microbiol Ind San Environ 2012;6:192-209.

Oumaskour K, Boujaber N, Assobhei O, Etahiri S. Anti-phospholipase A2 and anti elastase Activities of sixteen marine green and brown algae from the the coast of el jadida morocco. J Chem Biol Phys Sci 2015;6:101-8.

Tuney I, Hilal Cadirci B, Unal D, Sukatar A. Antimicrobial activities of the extracts of marine algae from the coast of Urla (Izmir, Turkey). Turk J Biol 2006;30:171-5.

Karabay Yavasoglu NU, Sukatar A, Ozdemir G, Horzum Z. Antimicrobial activity of volatile components and various extracts of the red alga Janiarubens. Phytother Res 2007;21:153-6.

Salvadora P, Begona Arti N, Querolb X, Alastueyb A, Costoyac M. Characterisation of local and external contributions of atmospheric particulate matter at a background coastal site. Atmos Environ 2007;41:1-17.

Boujaber N, Oumaskour K, Assobhei O, Etahiri S. Preparation of an antifungal soap from Gelidium sesquipedale waste. Afr Biodivers Conserv 2014;44:9-1.

Lakhdar F, Boujaber N, Oumaskour K, Assbhei O, Etahiri S. Inhibitive activity of 17 marine algae from the coast of El Jadida-Morocco against Erwinia chrysanthemi. Int J Pharm Pharm Sci 2015;7:376-80.

Mayer AMS. Marine pharmacology in 2003-4: marine compound with anthelmintic, antibacterial, anticoagulant, antifungal, anti-inflammatory, antimalarial, antiplatelet, antiprotozoal, antituberculosis and antiviral activities; affecting the cardiovascular, immune and nervous systems, and other miscellaneous mechanisms of action. Comp Biochem Phys 2007;145:553-81.

Samri N, Hsaine L, El Kafhi S, Khlifi S, Etahiri S. Radical scavenging activity and phenolic contents of brown seaweeds harvested from the coast of sidi bouzid (El Jadida, Morocco). Int J Pharm Sci Rev Res 2019;54:116-22.

Hsaine L, Samri N, Elkafhi S, Etahiri S, Khlifi S. Phenolic compounds and radical scavenging activity of red seaweeds harvested from the atlantic coast of sidi bouzid morocco. Int J Pharm Sci Rev Res 2019;56:73-81.

Hamouche E, Sarkis DK. Evolution of susceptibility to antibiotics of Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumanii, in a University Hospital Center of Beirut between 2005 and 2009. Pathol Biol 2012;60:15-20.

Etahiri S, Bultel Ponce V, Caux C, Guyot M. New bromoditerpenes from the red alga Sphaerococcus coronopifolius. J Nat Prod 2001;64:1024-7.

Etahiri S, El Kouri A, Bultel Ponce V, Guyot M, Assobhei O. Antibacterial bromophenol from the marine red alga Pterosiphonia complanata. Nat Prod Com 2007;2:749-52.

Caccamese S, Azzolina R. Screening for antimicrobial activities in marine algae from Eastern Sicily. Med Plant Nat Prod Res 1979;37:333-9.

Bauer AW. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.

Del Val AG, Platas G, Basilio A, Cabello A, Gorro Chateui J, Suay I, et al. Screening of antimicrobial activities in red, green and brown macroalgae from gran canaria (Canary Islands, Spain). Int Microbiol 2001;4:35-40.

Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 2007;42:321-4.

Ara J, Sultana V, Ehteshamul Haqye S, Athar M, Qasim R. Antibacterial activity of marine macro-algae from Karachi coast. B Pol Acad Sci 2002;50:199-206.

Etahiri S, Butel Ponce V, EL kouri AK, Assobhei O, Zaoui D, Guyot M. Antibacterial activity of marine algae from the atlantic coast of Morocco. Mar Life 2003;13:3-9.

Chiheb I, Riadi H, Martine Lopez J, Dominguez Seglar JF, Gomez Vidal JA, Bouziane H, et al. Screening of antibacterial activity in marine green and brown macroalgae from the coast of Morocco. Int Afr J Biotech 2009;8:1258-562.

Bennamara A, Abourriche A, Berrada M, Charrouf M, Chaib N, Boudouma M, Garneau FX. Methoxybifurcarenone: an antifungal and antibacterial meroditerpenoid from the brown alga Cystoseiratamariscifolia. Phytochemistry 1999;52:37-40.

Sastry A, Rao P. Antibacterial substances from marine algae: successive extraction using Benzene, chloroform and methanol. Bot Mar 1994;37:335-60.

Cox S, Abu Ghannam N, Gupta S. An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds. Int Food Res J 2010;17:205-20.

Rani V, Jawahar P, Jeya R, Shakila A, Srinivas AN. Antibacterial activity of some brown seaweeds of gulf of mannar, South East Coast of India. J Pharm Bio Sci 2016;4:14-21.

Perez RM, Avila JG, Perez S, Martinez A, Martinez G. Antimicrobial activity of some American algae. J Ethnopharmacol 1990;29:111-6.

Vidyavathi N, Sridhar KR. Seasonal and geographical variations in the antimicrobial activity of seaweeds from the Mangalore coast in India. Bot Mar 1991;34:279-84.

Zheng Yi, Chen Yi, Lu Ha. Screening for antibacterial and antifungal activities in some marine algae from the Fujian coast of China with three different solvents. Chin J Oceanol Limn 2001;19:327-31.

Farid Y, Etahiri S, Assobhei O. Activité antimicrobienne des algues marines de la lagune d’Oualidia (Maroc): criblage et optimisation de la periode de recolte. J Appl Biosci 2009;24:1543-52.

Hornesy IS, Hide D. The production of antimicrobial compounds by British Marine algae. IV variation of antimicrobial activity with algal generation. J Br Psychol 1985;20:21-5.

Patra JK, Patra AP, Mahapatra NK, Thatoi HN, Das S, Sahu RK, et al. Antimicrobial activity of organic solvent extracts of three marine macroalgae from Chilika Lake, Orissa, India. Malays J Microbiol 2009;5:128-31.

Alves C, Pinteus S, Simoes T, Horta A, Silva J, Tecelao C, et al. Bifurcaria bifurcata: a key macro-alga as a source of bioactive compounds and functional ingredients. Int J Food Sci Technol 2016;5:1638-46.

Rizzo C, Genovese G, Morabito M, Faggio C, Pagano M, Spano A, et al. Potential antibacterial activity of marine macroalgae against pathogens relevant for aquaculture and human health. J Pure Appl Microbiol 2017;11:1695-706.

Wagih A, El-shouny Reda M, Gaafar Gehan A, Ismail Marwa M, Elzanaty M. Seasonal variation of the antibacterial activity of some seaweeds against multi drug resistant pathogenic bacterial strain. Egypt J Exp Biol 2017;13:341-51.

Bouhraoua J, Lakhdar F, Mabrouki S, Benba J, Assobhei O, Etahiri S. Antifungal activity of 23 seaweeds from the coast of el jadida morocco against Fusarium culmorum and Alternria alternate. Int J Pharm Sci Rev Res 2018;51:53-60.

Erturk O, Taş B. Antibacterial and antifungal effects of some marine algae. J Faculty Veterinary Med Kafkas University 2011;17:121-4.

Oumaskour K, Boujaber N, Etahiri S, Assobhei O. Screening of antibacterial and antifungal activities in green and brown algae from the coast of Sidi Bouzid (El Jadida, Morocco). Afr J Biotech 2012;11:16831-7.

Grozdanic N, Kosanic M, Etahiri S, Assobhei O, Kljajic Z, Stanojkovic T. An insight into cytotoxic and antimicrobial effects of Cystoseira humilis crude extract. Stud Mar 2011;29:21-30.

Sonia A, Santos O, Stephanie ID, Trindade S, Catia S, Oliveira D, et al. Lipophilic fraction of cultivated Bifurcaria bifurcata. R. ross: detailed composition and in vitro prospection of current challenging bioactive properties. Mar Drugs 2017;15:340.

Alves C, Pinteus S, Simoes T, Horta A, Silva J, Tecelao C, et al. Bifurcaria bifurcata: a key macro-alga as a source of bioactive compounds and functional ingredients. Int J Food Sci 2016;51:1638-46.

Saleh B, Al-Mariri A. Antifungal activity of crude seaweed extracts collected from latakia coast, Syria. J Fish Aquat Sci 2018;13:49-55.