COMPARATIVE STUDY OF ANTICONVULSANT EFFECT OF THE LEAVES OF SAPINDUS EMARGINATUS AND ACORUS CALAMUS IN EXPERIMENTALLY INDUCED ANIMAL MODELS OF EPILEPSY

PRIYADARSHINI SHOUGRAKPAM; ABHISHEK BHATTACHARJEE; NGANGOM GUNINDRO; SANJENBAM RITA

doi:10.22159/ijpps.2021v13i1.39872

Authors

PRIYADARSHINI SHOUGRAKPAM Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, India 795004
ABHISHEK BHATTACHARJEE Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, India 795004
NGANGOM GUNINDRO Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, India 795004
SANJENBAM RITA Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, India 795004

DOI:

https://doi.org/10.22159/ijpps.2021v13i1.39872

Keywords:

Anticonvulsant, Maximal electric shock, Pentylenetetrazol, Sapindus emarginatus, Acorus calamus

Abstract

Objective: To compare anticonvulsant activity of methanol extracts of Sapindus emarginatus (MESE) and Acorus calamus (MEAC) in experimental seizure models in mice.

Methods: Hind limb tonic extension (HLTE) in Maximal electroshock (MES) seizure and clonic seizure in Pentylenetetrazol (PTZ) seizure models were assessed. Group I (control) mice received 1% gum acacia in distilled water (1 ml/100 g). Topiramate (50 mg/kg) was administered in group II (standard) animals. Group III and IV mice were treated with 200 and 400 mg/kg of MESE, respectively. Mice in group V and VI were given MEAC at the dose of 200 and 400 mg/kg, respectively. Drugs were given orally suspended in 1% gum acacia suspension (1 ml/100 g) for 7 d. Next day after 1 h of drug administration, the seizure was induced for evaluation.

Results: Anticonvulsant property of both extracts was confirmed by reduction (p<0.001) in HLTE phase in MES model; delayed onset of the clonic seizure (p<0.001) and its shortened phase (p<0.001) in PTZ model when compared with the control. MESE-200 mg/kg produced significantly longer (p<0.001) HLTE phase with lower protection (40.34%) among the different doses of the extracts. Clonic seizure onsets and durations in PTZ model were comparable among the different extract-treated groups; however, mortality was higher (66.6%) with MESE-200 mg/kg.

Conclusion: Anticonvulsant activity of MESE and MEAC was evident; however, MESE at the dose of 200 mg/kg was less effective.

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References

Wahab A. Difficulties in treatment and management of epilepsy and challenges in new drug development. Pharmaceuticals 2010;3:2090-110.

Engelborghs SD, Hooge R, De Deyn PP. Pathophysiology of epilepsy. Acta Neurol Belg 2000;100:201-13.

Stafstrom CE, Carmant L. Seizures and epilepsy: an overview for neuroscientists. Cold Spring Harb Perspect Med 2015;5:1-18.

Abdel-Rahman RF, Soliman GA, Yusufoglu HS, Tatli-Çankaya I, Alqasoumi SI, Anul SA, et al. Potential anticonvulsant activity of ethanol extracts of Cichorium intybus and Taraxacum serotinum in rats. Trop J Pharm Res 2015;14:1829-35.

Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 y. J Nat Prod 2007;70:461-77.

Hegde K, Thakker SP, Joshi AB, Shastry CS, Chandrashekhar KS. Anticonvulsant activity of Carissa carandas Linn. root extract in experimental mice. Trop J Pharm Res 2009;8:117-25.

Sulthana S, Naz S. Antiepileptic activity of Sapindus emerginatus vahl. fruit extract in pentylenetetrazol-induced seizure model. Int J Pharm Pharm Sci 2013;5(Suppl 1):280-4.

Shetty GR, Shruthi AM. A review on pharmacology of Acorus calamus-an an endangered medicinal plant. Int J Pharm Bio Sci 2015;6:605-21.

Lin J, Opoku AR, Keller MG, Hutchings AD, Terblanche SE, Jager AK, et al. Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities. J Ethnopharmacol 1999;68:267-74.

Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 45th ed. Pune: Nirali Prakashan; 2009.

Shah B, Seth AK. Textbook of pharmacognosy and phytochemistry. 2nd ed. New Delhi: Elsevier; 2014.

OECD. Test No. 423: Acute oral toxicity-acute toxic class method, OECD guidelines for the testing of chemicals, Section 4, OECD Publishing, Paris; 2002. Available from: https://doi.org/ 10.1787/9789264071001-en. [Last accessed on 18 Aug 2018].

Castel Branco MM, Alves GL, Figueiredo IV, Falcao AC, Caramona MM. The maximal electroshock seizure (MES) model in the preclinical assessment of potential new antiepileptic drugs. Methods Find Exp Clin Pharmacol 2009;31:101-6.

Kulkarni SK. Practical pharmacology and clinical pharmacy. Delhi: Vallabh Publications; 2008.

Gupta SK. Drug screening methods. 3rd ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd; 2016.

Lahon J, Phukan S, Sharma U. Study of the anticonvulsant potential of leaves of Clitoria ternatea linn. In pentylenetetrazole and maximal electroshock seizure-induced convulsions in experimental animals. Asian J Pharm Clin Res 2016;9:258-61.

Remy M. Why do medical researchers use mice? Live Sci; 2010. Available from: http://www.livescience.com/32860-why-do-medical-researchers-use-mice.html. [Last accessed on 12 Jul 2019].

Vogel HG, Hock FJ, Maas J, Mayer D. editors. Drug discovery and evaluation: Safety and pharmacokinetic assays. New York: Springer-Verlag Berlin Heidelberg; 2013.

White HS. Clinical significance of animal seizure models and mechanism of action studies of potential antiepileptic drugs. Epilepsia 1997;38(Suppl I):S9-S17.

Mandhane SN, Aavula K, Rajamannar T. Timed pentylenetetrazol infusion test: a comparative analysis with s. c. PTZ and MES models of anticonvulsant screening in mice. Seizure 2007;16:636-44.

Czapinski P, Blaszczyk B, Czuczwar SJ. Mechanisms of action of antiepileptic drugs. Curr Top Med Chem 2005;5:3-14.

Khoshnoud MJ, Tanideh N, Namdarian S. Anticonvulsant activity of atorvastatin against seizure-induced by pentylenetetrazole and maximal electroshock in mice. Trends Pharmacol Sci 2015;1:44-7.

Chindo BA, Anuka JA, McNeil L, Yaro AH, Adamu SS. Amos S, et al. Anticonvulsant properties of saponins from Ficus platyphylla stem bark. Brain Res Bull 2009;78:276–82.

Singh P, Singh D, Goel RK. Phytoflavonoids: antiepileptics for the future. Int J Pharm Pharm Sci 2014;6:51-66.

Kamboj P, Singh I, Mahadevan N, Chaudhary G. Anticonvulsants from nature. Pharmacogn Rev 2009;3:108-17.