BIO-POTENTIATION AND IN VIVO PHARMACOKINETICS STUDIES OF POLYPHENOLS FROM PUNICA GRANATUM BY FORMING PHOSPHOLIPID CONJUGATES FOR BETTER ANTIOXIDANT & ANTI-INFLAMMATORY ACTIVITY

Authors

  • Deep Bhattacharya Molecular Simulations Group, Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, University of Mumbai, Kalina, Santacruz [East], Mumbai 400098. India.
  • Priscilla M D’mello Prin. K. M. Kundnani, College of Pharmacy
  • Tarun Kumar Dasgupta Prin. K. M. Kundnani, College of Pharmacy

Keywords:

Punica granatum, Flavonoids, In vivo, Glucuronide

Abstract

Objective: Polyphenols belong to the chemical class of flavonoids and are widely distributed in vegetables and plants. In this study, we aimed to potentiate the antioxidant and anti-inflammatory effects of polyphenols by forming a phospholipid complex of extracts from Punica granatum and also evaluating its in vivo pharmacokinetic parameters.

Methods: This method was used for determination and in vivo pharmacokinetics studies for the biomarkers and their lipid complexes from P. granatum extract. Anti-inflammatory and antioxidant studies ever performed on both uncomplexed and complexed flavonoids. Pharmacokineticbehaviour of biomarker was significantly improved with higher Cmax, t1/2 and shorter tmax& AUC0-t and AUC0-t values of glucuronide metabolites indicating the greater bioavailability.

Results: Anti lipid peroxidation inhibitory potential was found greater in case of phospholipid complexes with IC50 value 17.41 µg for biomarker complex as compared to biomarker mixture (63.52 µg) & anti-inflammatory activity of 100 mg/kg of agro waste extract complex of P. granatum was found very significant at 3rd hour with (21.95±0.071 %) inhibition in rat paw edema which was greater than the extract alone (11.13±0.084 %).

Conclusion: Bio-enhancement of polyphenols was observed and achieved through phospholipids complexes and was demonstrated by in vitro and in vivo activity testing by forming a supramolecular complex.

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Author Biography

Deep Bhattacharya, Molecular Simulations Group, Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, University of Mumbai, Kalina, Santacruz [East], Mumbai 400098. India.

Molecular Simulations Group, Department of Pharmaceutical Chemistry, Bombay College of Pharmacy, University of Mumbai, Kalina, Santacruz [East], Mumbai 400098. India.

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Published

01-03-2015

How to Cite

Bhattacharya, D., P. M. D’mello, and T. K. Dasgupta. “BIO-POTENTIATION AND IN VIVO PHARMACOKINETICS STUDIES OF POLYPHENOLS FROM PUNICA GRANATUM BY FORMING PHOSPHOLIPID CONJUGATES FOR BETTER ANTIOXIDANT & ANTI-INFLAMMATORY ACTIVITY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 164-72, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/4117.

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