INTERACTION BETWEEN CHOLINERGIC AND OPIOID SYSTEMS IN RELAPSE TO ALCOHOL DEPENDENCE
Keywords:
Alcohol, Morphine, Conditioned place preference, Relapse, AcetylcholinesteraseAbstract
Objective: To confirm the interaction between cholinergic and opioid systems in alcohol dependence using an animal model.
Methods: Experiments were conducted using Conditioned Place Preference (CPP) paradigm. Mice were conditioned with alcohol, nicotine and combination of both. They were then subjected to postconditioning test, in which their preference scores were measured. Following a period of drug abstinence, they were reinstated by morphine at doses of 5, 10, 20 and 40 mg/kg BW to induce relapse. Acetylcholinesterase (AChE) activity measurements were performed at the end of the behavioral tests using Ellman's method.
Results: Priming dose of morphine of 10 mg/kg, 20 mg/kg and 40 mg/kg  BW  increased significantly the preference score during relapse to alcohol compared with the score in post conditioning test. AChE activity in animal at the time of relapse was significantly different compared to saline treated group. The highest enzyme activity was shown after priming dose of 20 mg/kg BW in group conditioned with alcohol and nicotine. There were no significant differences between the activity of AChE in groups receiving 5 mg/kg, 10 mg/kg, 20 mg/kg and 40 mg/kg BW of morphine challenge.
Conclusion: Result of the present study indicated that morphine challenge in alcohol dependent animals during drug abstinence induces relapse to alcohol dependence. This is accompanied by increased AChE activity suggesting cholinergic-opioid system interaction.
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References
Alwan A. Global status report of alcohol and health. WHO 2011:25-9.
Drews E, Zimmer A. Modulation of alcohol and nicotine respone through the endogenous opioid system. Prog Neurobiol 2009;90:1-15.
Davis TJ, de Fiebre CM. Alcohol’s action on neural nicotinic acetylcholine receptors. Biol Mech 2006;29(3):179-85.
Valiki A, Tayebi K, Jafari MR, Zarrindast MR, Djahanguiri B. Effect of ethanol on morphine state dependent learning in the mouse: involvement of gabaergic, opioidergic and cholinergic system. Alcohol and Alcoholism 2004;39(5):427-30.
Laurence L Brunton, Keith L Parker, editor. Goodman and gilman, manual of pharmacology and therapeutics. 8th ed. San Diego: Mc Graw Hill Company; 2008.
Marinelli PW, Quirion R, Gianoulakis C. An In vivo profile of beta-endorphin release in the arcuate nucleus and nucleus accumbens folowing exposure to stress or alcohol. Neurosci 2004;127:777-84.
Isola R, Zhang H, Tejwani GA, Neff NH, Hadjiconstantinou M. Acute nicotine change dynorphin and prodynorphin mRNA in the striatum. Psychopharm 2009;201:507-16.
Alexander G Karczmar, editor. Exploring the vertebrate central cholinergic nervous system. 1st ed. Berlin: Springer; 2007.
Trudeau S, Cartier GS. Biochemical methods to determined cholinesterase activity in wildlife expose to pesticides. national wildlife research center canadian wildlife service. Tech Rep Ser 2000;338:2-5.
Gerard Vogel H, editor. Drug discovery and evaluation, pharmacological assay. 3rd ed. New York: Springer-Verlag Berlin Heidelberg; 2008.
Ellman GL, Courtney KD, Andreas V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharm 1960;7:88-90.
Cunningham L, Groblewski PA, Voorhees CM. Place conditioning, animal models of drug addiction. Neuromethods 2011;53:167.
Rezayof A, Serenjeh FN, Zarrindast MR, Sepheri H, Delphi L. Morphine-induce place preference, involvement of cholinergic receptors of ventral tegmental area. Eur J Pharmacol 2007;562:92-102.
Le AD, Li Z, Funk D, Sharm M, Li TK, Shaham Y. Increase vulnerability to nicotine self-administration and relapse in alcohol-naive offspring of rats selectively bred for high alcohol intake. J Neurosci 2006;26(6):1872-9.
Le AD, Wang A, Harding S, Juzytsch W, Shaham Y. Nicotine increase alcohol self-administration and reinstates alcohol seeking in rats. Psychopharm 2003;168:216-21.
Moreno JAP, Trigo-Diaz JM, de Fonseca FR, Cuevas GG. Nicotine in alcohol deprivation increases alcohol operant self-administration during reinstatement. Neuropharmacol 2004;47:1036-44.
Taylor DH, Steffensen SC, Wu J. Nicotinic acetylcholine receptors in ventral tegmental area are important targets for nicotine and ethanol co-dependence. Biochem Pharmacol 2013;13:1-6.
Cami J, Farre M. Mechanism of disease, drug addiction. New Engl J Med 2003;349(10):979-80.
Clapp P, Bhave SV, Hoffman PL. How adaptation of the brain to alcohol leads to dependence. Alcohol Res Health 2008;31(4):311-24.
Olive MF, Koenig HN, Nannini MA, Hodge CW. Stimulation of endorphin neurotransmission in the nucleus accumbens by ethanol, cocaine, and amphetamine. Neurosc 2001;21(23):1-5.
Xiao C, Zhang J, Krnjevic K, Ye JH. Effects of ethanol on midbrain neurons: role of opioid receptors. Alcoholism Clin Exp Res 2007;31(7):1106-13.
Johnson SW, North RA. Opioid excite dopamine neurons by hyperpolarization of local interneurons. J Neurosci 1992;12(2):483-8.
Marco EM, Granstrem O, Moreno H, Llorente R, Adriani W, Laviola G, Viveros MP. Subchronic nicotine exposure in adolesence induce long-term effects on hippocampal and striatal cannabinoids-CB1 and µ-opioid receptors in rats. Eur J Pharmacol 2007;557(1):37-43.
Wewers ME, Dhatt RK, Snively TA, Tejwani GA. The effect of chronic administration of nicotine on antinociceptive, opioid receptors binding and met-enkelphalin level in rats. Brain Res 1999;822(2):107-13.
Huston JP, Silva MAS, Topic B, Muller CP. What’s conditioned in conditioned place preference?. Pharm Sci 2013;34(3):163-4.
Quirk GJ. Memory for extinction of conditioned fear is long-lasting and persist following spontaneous recovery. Learn Mem 2002;9:402-7.
Hyman SE, Malenka RC, Nestler EJ. Neural mechanism of addiction: the role of reward-related learning and memory. Annu Rev Neurosci 2006;29:565-98.
Acheson SK, Ross EL, Swartzwelder HS. Age-independent and dose-response effect of ethanol on spatial memory in rats. Alcohol 2001;23:167-75.
Henn C, Klein J, Loffelholz K. Stimulatory Influence of Ethanol on the septohippocampal cholinergic pathway. A role for GABA receptors. J Physio 1998;92:439-40.
Prediger RD, Takahashi RN. Ethanol improves short-term social memory in rats. involvement of opioid and muscarinic receptors. Eur J Pharmcol 2003;462:115-23.
Meilandt WJ, Rodriguez EB, Harvey SAK, Martinez Jr JL. Role of Hippocampal CA3µ-Opioid receptors in spatial learning and memory. Neurosci 2004;24(12);2953-62.
Taraschenko OD, Rubbinaccio HY, Shulan JM, Glick SD, Maissonneuve IM. Morphine-induce change in acetylcholine release in the interpenducular nucleus and relationship to changes in motor behaviour in rats. Nat Inst Health 2007;53(1):18-26.
Schoffelmeer ANM, Hongenboom F, Mulder A. κ1-and κ2-Opi id receptors mediating presynaptic inhibition of dopamine and acetylcholine release in rat neostriatum. Br J Pharmcol 1997;122:523.