STANDARDIZATION OF FRIABLE CALLUS DEVELOPMENT IN CATHARANTHUS ROSEUS (LINN.) G. DON
Keywords:
Callus induction, Friable callus, Catharanthus roseusAbstract
Objective: The objective of the study was to develop an effective hormonal combination for the maximum growth of callus and development of friable calli using the same medium with reduced concentration of agar.
Methods: The percentage responses of five varied growth hormonal combinations and concentrations, supplemented with Murashige and Skoog (MS)medium were recorded. The effect of casein hydrolysate on callus induction was also studied. The nature of friable calli obtained from best responsive media fortified with 0.7% and 0.6% agar was observed.
Results: The present study revealed that, three media viz., MS + 1.0 mg/L BAP + 1.0 mg/L NAA, MS + 1.5 mg/L 2,4-D + 1.0 mg/L Kin and MS + 1.5 mg/L 2,4-D + 0.5 mg/L BAP, as the best responsive media in the descending order. The effect of casein hydrolysate supplemented along with the above three media revealed MS + 1.0 mg/LBAP + 1.0 mg/L NAA + 1.0 gm/L casein hydrolysate as the best responsive media. Also, the above media supplemented with 0.6% agar was found to be the effective in terms of nature and amount of friable callus obtained.
Conclusion: The results indicated MS + 1.0 mg/L BAP + 1.0 mg/L NAA + 1.0 gm/L casein hydrolysate + 0.6% agar (85% response) as the best media for the growth and development of both callus and friable callus.
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Ashutosh KV, Singh RR, Seema S. Improved alkaloid content in callus culture of Catharanthus roseus. Bot Serbica 2012;36(2):123-30.
Kiruba S, Mahesh M, Nisha SR, Miller PZ, Jeeva S. Phytochemical analysis of the flower extracts of Rhododendron arboretum Sm. Ssp. Nilagiricum (Zenker) Tagg. Asian Pac J Trop Biomed 2011;284-6.
Tirupathi RG, Suresh BK, Ujwal KJ, Sujana P, Veerabhadr R, Sreedhar AS. Anti-microbial principles of selected remedial plants from southern India. Asian Pac J Trop Biomed 2011;1(4):298-305.
Anpin Raja RD, Jeeva S, Prakash JW, Johnson M, Irudayaraj V. Antibacterial activity of selected ethnomedicinal plants from South India. Asian Pac J Trop Biomed 2011;4(4):375-8.
Mathew R, Sankar PD. Plant cell culture technology and its entrée into the world of Ocimum. Int J Pharm Pharm Sci 2013;5 Suppl 2:6-13.
Soon HT, Chung YL, Hazrina H, Aditya A, Mohammadjavad P, Won FW, et al. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Mol 2013;18:9770-84.
Jaleel CA, Gopi R, Manivannan P, Sankar B, Kishorekumar A, Panneerselvam R. Antioxidant potentials and ajmalicine accumulation in Catharanthus roseus after treatment with giberellic acid. Colloids Surf B 2007;60:195-200.
Renu S, Pushpa K, Kanta R. Rapid micropropagation and callus induction of Catharanthus roseusin vitro using different explants. World J Agric Sci 2011;7(6):699-704.
Bakrudeen AAA, Subha Shanthi G, Gouthaman T, Kavitha MS, Rao MV. In vitro micropropagation of Catharanthus roseus–An anticancer medicinal plant. Acta Bot Hung 2011;53(1-2):197-209.
Jitendra M, Deeksha U, Priyanka P, Rukhshar A, Sunil R, Shalini T. Multiple shoots regeneration of (anti-cancer plant) Catharanthus roseus–An important medicinal plant. Am J Pharm Tech Res 2013;3;1.
Mohammed I, Syeda Sughra Mehjabeen, Mangamoori LN. Pharmacological evaluation of Catharanthus roseus. Int J Pharm Appl 2011;2(3):165-73.
Rupesh KR, Naresh KS, Vandana S. Effect of plant growth regulators on micropropagation of Catharanthus roseus. Int J Adv Biotechnol Res 2013;4(1):123-30.
Van der HR, Jacobs DI, Snoeijer W, Hallard D, Verpoorte R. The Catharanthus roseus alkaloids: pharmacognosy and biotechnology (a review). Curr Med Chem 2004;11:607-28.
Magnotta M, Murata J, Chen J, De LV. Identification of a low vindoline accumulating cultivar of Catharanthus roseus (L.) G. Don. by alkaloid and enzymatic profiling. Phytochem 2006;67:1758-64.
Jaleel CA, Gopi R, Sankar B, Manivannan P, Kishorekumar A, Sridharan R, et al. Alterations in germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedlings under salt stress. South Afr J Bot 2007;73:190-5.
Akira I, Hideki A, Masaru OT, Hideo T. Development of a novel system for producing ajmalicine and serpentine using direct culture of leaves in Catharanthus roseus intact plant. J Biosci Bioeng 2005;99(3):208-15.
Agnieszka P, Miroslawa F, Barbara L. Catharanthus roseus: micropropagation and in vitro techniques. Phytochem Rev 2007;6:459-73.
Sidhu Y. In vitro micropropagation of medicinal plants by tissue culture. Plymouth Student Sci 2010;4(1):432-49.
Rukhama H, Shagufta N, Farah A, Farkhanda M. Comparison of in vitro response of micropropagation and callogenesis of medicinal plant, Vinca rosea L. J Agric Res 2013;51(1):9-17.
Taha HS, El-Bahr MK, El-Nasr MMS. In vitro studies on Egyptian Catharanthus roseus (L.) G. Don: Calli production, direct shootlets regeneration and alkaloids determination. J Appl Sci Res 2008;4(8):1017-22.