ANTIDIABETIC EFFECT AND PHYTOCHEMICAL SCREENING OF ETHANOLIC EXTRACT OF POLYALTHIA CERASOIDES STEM BARK IN STREPTOZOTOCIN INDUCED DIABETIC ALBINO RATS
Keywords:
Polyalthia cerasoides, Antihyperglycemic, Antidiabetic, Streptozotocin, Glibinclamide, HistopathologyAbstract
Objective: Polyalthia cerasoides stem bark is used in treatment the of various diseases. Some tribes of North Orissa used the stem bark in the treatment of diabetes. We investigated the effect of four different solvent (n-hexane, ethyl acetate, ethanol and aqueous) extracts of Polyalthia cerasoides stem bark on streptozotocintozocin (STZ) 45 mg/kg b. wt. induced diabetic rats.
Methods: Normal control and diabetic control groups were allowed with free access to water throughout the experiment. Oral administration of four different solvent (n-hexane, ethyl acetate, ethanol and aqueous extracts of P. cerasoides stem bark to 12 hrs fasted normal and STZ induced diabetic rats at a dose of 200, 400 and 600 mg/kg b. wt for acute studies. (Determination of effective extract and dose: Â Each group was maintained with six rats)
The fasting blood glucose FBG levels were checked for every 2 hrs (1h, 3h, 5h & 7h). Chronic study: Â (Each group was maintained with six rats)
The normal and diabetic groups were supplemented with optimum dosage 400mg ethanol extract of P. cerasoides (PcEE) stem bark and 20 mg of glibinclamide (Glb) a standard drug treated diabetic group for 21 days.
Results: Acute administration of P. cerasoides stem bark reduced FBG[A1]Â levels in ethanol extract atthe dosee 400 and 600 mg/ kg b. wt (48.5% and 32.4%, P<0.05) in the diabetic rats only. It does not affect the normal FBG levels in normal rats. In chronic treatment significant reduction in FBG levels in diabetic rats (51.6%). Body weight also increased in extract treated animals. The maximal antidiabetic effect was obtained in 400 mg PcEE[A2]Â which was similar to Glb treated group. The PcEE treated diabetic rats also confirmed the significant recovery of liver and kidney destruction.
Conclusion: Our study has revealed the therapeutic effect of PcEE for diabetes and its related complications.
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References
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