INVESTIGATION OF ANALGESIC ACTIVITY OF XANTHINE OXIDASE INHIBITOR ALLOPURINOL: AN EXPERIMENTAL STUDY

Authors

  • Pise H. N. Assistant Professor, SRTR Govt. Medical College, Ambajogai. Dist-Beed, Maharashtra.
  • Padwal S. L. SRTR Govt. Medical College
  • Motghare V. M. SRTR Govt. Medical College
  • Deshmukh V. S. SRTR Govt. Medical College
  • Jaykare S. C. SRTR Govt. Medical College
  • Jadhav S. S. SRTR Govt. Medical College

Keywords:

Analgesic, Allopurinol, Purines, Tail-flick method, Xanthine oxidase inhibitor

Abstract

Objectives: To investigate the analgesic activity of xanthine oxidase inhibitor, allopurinol in three graded doses and compare it with control and aspirin.

Methods: Analgesic effect of allopurinol in three graded doses (100, 200, 400 mg/kg I. P.), aspirin in analgesic dose (100mg/kg I. P.) and tramadol was evaluated by using Radiant heat Tail-flick method and Acetic-acid induced writhing method in albino rats and mice respectively. The results were analyzed statistically by ANOVA followed by Dunnet's test.

Results: Allopurinol possesses significant analgesic activity in both models of analgesia. In Tail-flick method, in doses of 100mg/kg the percentage of tail flick elongation time with allopurinol is comparable with aspirin at all time intervals with maximum activity at 60 minutes. In doses of 200mg/kg, the percentage increase in reaction time is more than aspirin at 30, 90 & 120 minutes but this difference is not statistically significant. In doses of 400mg/kg, the percent increase in reaction time is more than aspirin but the difference is not statistically significant.

In acetic acid induced writhing method, allopurinol in dose of 100mg/kg differ significantly than aspirin, latter being more effective. The analgesic activity of allopurinol in doses of 200mg/kg & 400mg/kg was comparable to aspirin.

Conclusion: Allopurinol possesses significant analgesic activity comparable with aspirin in tail flick model as well as acetic acid induced model of nociception. And this drug should be further evaluated for this indication

 

Downloads

Download data is not yet available.

Author Biography

Pise H. N., Assistant Professor, SRTR Govt. Medical College, Ambajogai. Dist-Beed, Maharashtra.

Dept. Of Pharmacology,

Assistant Professor

References

Ralevic V, Burnstock G. Receptors for purines and pyrimidines. Pharmacol Rev 1998;50:413-92.

Sawynok J. Adenosine receptor activation and nociception. Eur J Pharmacol 1998;317:1-11.

Bhardwaj A, Northington FJ, Koehler RC, Stiefel T, Hanley DF, Traystman RJ. Adenosine modulates N-Methyl D-Aspartate-stimulated hippocampal nitric oxide production in vivo. Stroke 1995;26:1627-33.

Kittleson MM, Hare JM. Xanthine oxidase inhibitors: an emerging class of drugs for heart failure. Eur Heart J 2005;26:1458-60.

Day RO, Graham GG, Hicks M, McLachlan AJ, Stocker SL, William KM. Clinical Pharmacikinetics and Pharmacodynamics of allopurinol and oxypurinol. Clin Pharmacokinet 2007;46(8):623-44.

Schmidt AP, Böhmer AE, Antunes C, Schallenberger C, Porciúncula LO, Elisabetsky E, et al. Analgesic properties of xanthine oxidase inhibitor allopurinol in mice: role of A1 adenosine receptor. Br J Pharmacol 2009;156(1):163-72.

D’Amour FE, Smith DL. A method for determining loss of pain sensation. J Pharmacol Exp Ther 1941;72:74-9.

Koster R, Anderson M, De-Beer EJ. Acetic acid for analgesic screening. Fed Proc 1959;18:412-8.

Parmar NS, Prakash S. Screening methods in pharmacology: Analgesic activity. New Delhi: Narosa Publishing House Pvt Ltd: 2006.

Sawynok J, Liu XJ. Adenosine in the spinal cord and periphery: release and regulation of pain. Progresia Neurobiol 2003;69:313-40.

Published

01-03-2015

How to Cite

H. N., P., P. S. L., M. V. M., D. V. S., J. S. C., and J. S. S. “INVESTIGATION OF ANALGESIC ACTIVITY OF XANTHINE OXIDASE INHIBITOR ALLOPURINOL: AN EXPERIMENTAL STUDY”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 173-6, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/4449.

Issue

Section

Original Article(s)