SPECTROSCOPIC ANALYSIS AND ANTIBACTERIAL EFFICACY OF BIOACTIVE COMPOUNDS FROM LIMONIA ACIDISSIMA L. FRUIT EXTRACT AGAINST CLINICAL PATHOGENS

Authors

  • Srinivasan Ponnuraj RESEARCH SCHOLAR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA
  • Dineshbabu Jaganathan RESEARCH SCHOLAR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA
  • Manimekalai Kanagarajan RESEARCH SCHOLAR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA
  • Teepica Priya Darsini Deivamarudachalam ASSOCIATE PROFESSOR, DEPT. OF BIOTECHNOLOGY, KARPAGAM UNIVERSITY, POLLACHI MAIN ROAD, COIMBATORE-21, TAMIL NADU, INDIA

Keywords:

Limonia acidissima, Phytochemical, Bioactive compounds, FT-IR, GC-MS, Antibacterial activity

Abstract

Objective: To determine the phytochemical composition using spectral analysis and antibacterial activity of Limonia acidissima fruit methanol extract.

Methods: Phytochemical analysis was performed to determine the total alkaloids, saponins, total phenols and flavonoids content of fruit extract. Secondary metabolite compounds were determined performed by FT-IR and GC-MS analysis. Antibacterial activity assay was performed using agar well diffusion technique.

Results: The alkaloid and saponins contents of the crude methanol extract were found to be 38.47 g/100 g and 0.13 g/100 g dry matter respectively. The LF methanol extract showed total phenol contents of 33.38 µg/mg and the flavonoids was found to be 33.80 µg/mg extract. FT-IR analysis indicated the presence of phenols, alkanes, amino acids, α, β-unsaturated esters, alkenes, nitro compounds, aromatics, aliphatic amines, carboxylic acid, alkenes, and alkyl halides functional groups. The GC-MS analysis revealed the presence of linoleic acid, octadecanoic acid, hexadecanoic acid, maltol, vinly guaiacol, furanone, and ascorbic acid. The antibacterial activity assay showed dose dependent inhibition against clinical pathogens.

Conclusion: This study elucidated the prospective value of L. acidissima fruit as a nutritional and medicinal source.


 

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References

Guarrera PM. Traditional phytotherapy in central italy (Marche, Abruzzo, and Latium). Fitoterapia 2005;76:1–25.

Matu EN, Van Staden J. Antibacterial and anti-inflammatory activities of some plants used for medicinal purposes in Kenya. J Ethnopharmacol 2003;87:35–41.

Robert CMJ. Discovering new antimicrobial agents. Int J Antimicrob Agents 2011;37:2–9.

Allen BM. Malayan Fruits. An introduction to cultivated species. Donald Moore Press Ltd. Singapore; 1967.

Khare CP. Indian medicinal plants: an illustrated dictionary, Springer Science, Springer Verlag, Berlin/ Heidelberg, Germany; 2007. p. 453.

Teepica Priya Darsini D, Maheshu V, Vishnupriya M, Nishaa S, Sasikumar JM. Antioxidant potential and amino acid analysis of underutilized tropical fruit Limonia acidissima L. Free Rad Antiox 2013;3(S):62-9.

Vidhya R, Narain A. Development of preserved products using under exploited fruit wood apple (Limonia Acidissima). Am J Food Technol 2011;6(4):279-88.

Yusuf M, Chowdhury JU, Wahab MA, Begum J. Medicinal plants of Bangladesh BCSIR Lab. Bangladesh; 1994. p. 62.

Rathayake RMRNK, Sumithra HJ, Fernando MD, Keerthi B, Palipane. Effect of GRAS compounds on Aspergillus rot of wood–apple (Feronia Limonia). Pytoparasit 2009;37:431-6.

Muniappan Ayyanar, Pandurangan Subash-Babu. Limonia acidissima Linn. (L.) Skeels: A review of its phytochemical constituents and traditional uses. Asian Pac J Trop Biomed 2012;240-6.

Senthilkumar KL, Kumawat KB, Rajkumar M, Senthilkumar. Anti-diarrhoeal activity of bark extracts of Limonia acidissima Linn. Res J Pharm Biol Chem 2010;1(4):550-3.

Bandara BMR, Gunatilaka AAL, Wijeratne EMK, Adikaram NKB. Antifungal constituents of Limonia acidissima. Planta Med 1988;54:374-5.

Rahman AA, Gopalakrishnan G, Ghouse BS, Arumugam S, Himalayan B. Effect of Feronia limonia on mosquito larvae. Fitoterapia 2000;71(5):553–5.

Kamat CD, Khandelwal KR, Bodhankar SL, Ambawade SD, Mhetre NA. Hepatoprotective activity of leaves of Feronia elephantum Correa (Rutaceae) against CCl4-induced liver damage in rats. J Nat Remed 2003;3:148-54.

Ahamed SM, Swamy SK, Jayaverra KN, Rao JV, Kumar VS. Anti-inflammatory antipyretic and analgesic activity of methanolic extract of Feronia limonia fruit pulp. Pharmacologyonline 2008;3:852-7.

Ilango K, Chitra V. Wound healing and anti-oxidant activities of the fruit pulp of Limonia acidissima Linn. (Rutaceae) in Rats. Trop J Pharm Res 2010;37:120-5.

Herborne JB. Phytochemical methods. Chapman and Hall London; 1973. p. 110−3.

Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extract of some homeostatic plants in Edo and Delta States of Nigeria. Global J Pure Appl Sci 2001;8:203−8.

McDonald S, Prenzler PD, Antolovich M, Robards K. Phenolic content and antioxidant activity of olive extract. Food Chem 2001;73:73-84.

Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10, 178-82.

Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med 2011;8(1):1-10.

Clinical and laboratory standards institute. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard. 7th ed. Clinical and Laboratory Standards Institute document M7-A7. Wayne, PA: Clinical and Laboratory Standards Institute; 2006.

Vaya J, Belinky PA, Aviram M. Antioxidant constituents from licorice roots: Isolation, structure elucidation and antioxidative capacity towards LDL Oxidation. Free Rad Biol Med 1997;23:302-13.

Shipra Pandey, Gouri Satpathy, Rajinder K Gupta. Evaluation of nutritional, phytochemical, antioxidant and antibacterial activity of exotic fruit Limonia acidissima. J Pharm Phytochem 2014;3(2):81-8.

Fu L, Xu BT, Xu X, Qin XU, Gan R, Li HB. Antioxidant capacities and total phenolic contents of 56 wild fruits from South China. Mol 2010;15:8602-17.

Veigas JM, Narayan MS, Laxman PM, Neelwarne B. Chemical nature, stability and bioefficacies of Anthocyanin from fruit peel of Syzygium cumini skeels. Food Chem 2007;105:619-27.

Havsteen BH. The biochemistry and medical significance of the flavonoids. Pharmacol Therap 2002;96:67-202.

Sandhya B, Thomas S, Isabel W, Shenbugarathai R. Ethnomedical plants used by the valaiyan community at Piranmalai Hills, Tamil Nadu, India. A pilot study. Afr J Trad Comp Alt Med 2006;3:101-14.

Muruganantham S, Anbalagan G, Ramamoorthy N. FT-IR and SEM-EDS comparative analysis of medicinal plants, Eclipta alba and Eclipta prostrate. Rom J Biophys 2009;19:285-94.

Das UN, Ramos EJ, Meguid MM. Metabolic alterations during inflammation and its modulation by central actions of omega-3 fatty acids. Curr Opin Clin Nutr Metab Care 2003;6:413–9.

Yong Q Chen. Modulation of prostate cancer genetic risk by omega-3 and omega-6 fatty acids. J Clin Invest 2007;117(7):1866–75.

Zhao YU, Peter VG, Irina AHe, Donghong W, Ting MV, Liliana G, et al. "Regulation of COX-2 Expression and IL-6 release by particulate matter in airway epithelial cells". Am J Respir Cell Mol Biol 2009;40(1):19–30.

Scorletti E, Byrne CD. Omega-3 Fatty Acids, Hepatic Lipid Metabolism, and Nonalcoholic fatty liver disease. Annu Rev Nutr 2013;33:231-48.

Pariza MW, Park Y, Cook ME. Mechanisms of action of conjugated linoleic acid: evidence and speculation. Proc Soc Exp Biol Med 2000;223:8–13.

O’Shea M, Bassaganya-Riera J, Mohede ICM. Immunomodulatory properties of conjugated linoleic acid. Am J Clin Nutr 2004;79(Suppl):1199S–206S.

Choi JS, Ha YM, Joo CU, Cho KK, Kim SJ, Choi IS. Inhibition of oral pathogens and collagenase activity by seaweed extracts. J Environ Biol 2012;33:115-21.

www.fao.org/ag/agn/jecfa-additives/specs/./Additive-182.pdf

Lasekan OO, Feijao JP, Salva TJG. Volatile flavour compounds of cooked acha†(Digitaria exilis). Food Chem 2001;15:131-6.

Hunter WJ, Manter DK, Van Der Lelie D. Biotransformation of ferulic acid to 4-vinylguaiacol by Enterobacter soli and E. aerogenes. Curr Microbiol 2012;65(6):752-7.

Koziol JM, Macia JM. Chemical composition, nutritional evaluation and economic prospects of Spondias purpurea (Anacardiaceae). Econ Bot 1998;52(4):373−80.

Patil SP, Kalkar SA, Kulkarni AS. Phytochemical Screening, Antibacterial and Antioxidant activity of Limonia acidissima (L). Bionano Front 2012;5(2):131-3.

Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 2009;22(1):102-6.

Sun CQ, O’Connor CJ, Roberton AM. Antibacterial actions of fatty acids and monoglycerides against Helicobacter pylori. FEMS Immunol Med Microbiol 2003;36:9-17.

Jae-Suk Choi, Nam-Hee Park, Seon-Yeong Hwang, Jae Hak Sohn, Inseok Kwak, Kwang Keun Cho, et al. The antibacterial activity of various saturated and unsaturated fatty acids against several oral pathogens. J Env Biol 2013;34:673-6.

Published

01-03-2015

How to Cite

Ponnuraj, S., D. Jaganathan, M. Kanagarajan, and T. P. D. Deivamarudachalam. “SPECTROSCOPIC ANALYSIS AND ANTIBACTERIAL EFFICACY OF BIOACTIVE COMPOUNDS FROM LIMONIA ACIDISSIMA L. FRUIT EXTRACT AGAINST CLINICAL PATHOGENS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 383-9, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/4711.

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