SCREENING OF CHITINASE PRODUCTION AND ANTIFUNGAL ACTIVITY OF STREPTOMYCES SP. ACT7 FROM EAST COAST REGION, SOUTH INDIA

Authors

  • D. Thirumurugan Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, Tamilnadu, India
  • D. Sankari SRM University
  • R. Vijayakumar Bharathidasan University Constituent College

Keywords:

East coast region, Sediments, Streptomyces, Chitinase, Biocontrol, Phytopathogens

Abstract

Objective: The objective of the present study was aimed to isolate actinobacteria from the marine environment and to screen the production and antifungal activity of chitinase enzyme against phytopathogenic fungi.

Methods: The actinobacteria were isolated from twelve sediment samples of East Coast Regions (ECR) by spread plate technique on starch casein agar, and the actinobacteria isolates were identified by the methods of International Streptomyces Project (ISP). All the actinobacteria were tested for chitinase activities using enrichment chitin agar media (EMCA) by spot inoculation method, and the potential isolate was used for chitinase production by submerged fermentation technique using 1% colloidal chitin in liquid medium. The efficacy of chitinase activity was assessed by spectrophotometric method along with growth monitoring. The most potential isolate Streptomyces sp. ACT7 was evaluated for its antagonistic activity against phytopathogens namely F. oxysporum and Alternaria sp. by dual culture method.

Results: Totally, 27 isolates of actinobacteria were isolated from marine sediments of ECR. Of them, 9 isolates had chitinase activity. Among the chitinase producers, the isolate ACT7 had prominent chitinase production after 7 days of incubation. The antagonistic activity of the isolate ACT7 was effective in restricting the radial growth of phytopathogens such as F. oxysporum and Alternaria sp.

Conclusion: The actinobacteria has found to possess potent antifungal activity and it can be used as a promising biocontrol agent, thereby reducing the use of chemical fungicides.

 

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Author Biography

D. Thirumurugan, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, Tamilnadu, India

Asst. Professor

Biotechnology

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Published

01-05-2015

How to Cite

Thirumurugan, D., D. Sankari, and R. Vijayakumar. “SCREENING OF CHITINASE PRODUCTION AND ANTIFUNGAL ACTIVITY OF STREPTOMYCES SP. ACT7 FROM EAST COAST REGION, SOUTH INDIA”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 5, May 2015, pp. 38-41, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/4823.

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