SCREENING OF CHITINASE PRODUCTION AND ANTIFUNGAL ACTIVITY OF STREPTOMYCES SP. ACT7 FROM EAST COAST REGION, SOUTH INDIA
Keywords:
East coast region, Sediments, Streptomyces, Chitinase, Biocontrol, PhytopathogensAbstract
Objective: The objective of the present study was aimed to isolate actinobacteria from the marine environment and to screen the production and antifungal activity of chitinase enzyme against phytopathogenic fungi.
Methods: The actinobacteria were isolated from twelve sediment samples of East Coast Regions (ECR) by spread plate technique on starch casein agar, and the actinobacteria isolates were identified by the methods of International Streptomyces Project (ISP). All the actinobacteria were tested for chitinase activities using enrichment chitin agar media (EMCA) by spot inoculation method, and the potential isolate was used for chitinase production by submerged fermentation technique using 1% colloidal chitin in liquid medium. The efficacy of chitinase activity was assessed by spectrophotometric method along with growth monitoring. The most potential isolate Streptomyces sp. ACT7 was evaluated for its antagonistic activity against phytopathogens namely F. oxysporum and Alternaria sp. by dual culture method.
Results: Totally, 27 isolates of actinobacteria were isolated from marine sediments of ECR. Of them, 9 isolates had chitinase activity. Among the chitinase producers, the isolate ACT7 had prominent chitinase production after 7 days of incubation. The antagonistic activity of the isolate ACT7 was effective in restricting the radial growth of phytopathogens such as F. oxysporum and Alternaria sp.
Conclusion: The actinobacteria has found to possess potent antifungal activity and it can be used as a promising biocontrol agent, thereby reducing the use of chemical fungicides.
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Surajit Das, Lyla PS, Ajmal Khan. Marine microbial diversity and ecology, importance and future perspectives. Curr Sci 2006;90:1325-34.
Dhanasekaran D, Panneerselvam A, Thajuddin N. Antifungal actinomycetes in marine soils of Tamil Nadu. Geobios 2005;32:37-40.
Mincer TJ, Jensen PR, Christopher AK, Fenical W. Widespread and persistent populations of a major new marine actinomycete taxon in ocean sediments. Appl Environ Microbiol 2002;68(3):5005-11.
Burja AM, Banaigs B, Abou-Mansour E, Burgess JG, Wright PC. Marine cyanobacteria-a prolific source of natural products. Tetrahedron 2001;57(6):9347-77.
Vijayakumar R, Panneerselvam K, Muthukumar C, Thajuddin N, Panneerselvam A, Saravanamuthu R. Antimicrobial potentiality of a halophilic strain of Streptomyces sp. VPTSA18 isolated from the salt pan environment of Vedaranyam, India. Annl Microbiol 2012;62:1039-47.
Nopakarn p, Abhinya P, Yano S, Marunu W, Takashi T. Utilization of shrimp, shellfish waste as a substrate for solid-state cultivation of Aspergillus sp. S-1-13. J Biosci Bioeng 2002;93:550-6.
Gohel V, Singh A, Vimal M, Ashwini P, Chhatapar HS. Review: Bioprospecting and antifungal potential of chitinolytic microorganisms. Afr J Biotechnol 2006;5:54-72.
Miyashita K, Fujii T, Sawada Y. Molecular cloning and characterization of chitinase genes from Streptomyces lividans 66. J Gen Microbiol 1991;137:2065-72.
Gupta R, Saxena RK, Chatuverdi P. Virdi JS. Chitinase production by Streptomyces viridificans: it’s potential in fungal cell wall lysis. J Appl Bacteriol 1995;78:378-83.
Joo JG. Purification and Characterization of an extracellular chitinase from the antifungal biocontrol agent Streptomyces halstedii. Biotechnol Lett 2005;27:1483-6.
Chuan GK. Environmental management. Singap J Trop Geogr 2006;13:14-24.
Williams ST, Shameemullah M, Watson ET, Mayfield CI. Studies on the ecology of the actinomycetes in soil, VI: The influence of moisture tension on growth and survival. Soil Biol Biochem 1972;4:215-25.
Ravel J, Amoroso MJ, Colwell RR, Hill RT. Mercury-resistant actinomycetes from the Chesapeake Bay. FEMS Microbiol Lett 1998;162:177-84.
Jensen PR, Dwight R, Fenical W. Distribution of actinomycetes in near shore tropical marine sediments. Appl Environ Microbiol 1991;57:1102-08.
Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16(3):313-40.
Das S, Lyla PS, Khan SA. Characterization and identification of marine actinomycetes existing systems, complexities and future directions. Natl Acad Sci Lett 2008;31(6):149-60.
Wiwat C, Siwayaprahm P, Bhumiratana A. Purification and characterization of chitinase from Bacillus circulans No. 4.1. Curr Microbiol 1999;39:134-40.
Hsu SC, Lockwood JL. Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. Appl Microbiol 1975;29:422-6.
Taechowisan T, Peberdy JF, Lumyong S. Chitinase production by endophytic Streptomyces aureofaciens CMU Ac 130 and its antagonism against phytopathogenic fungi. Ann Microbiol 2003;53:447-61.
Green F, Clausen CA, Highley TL. Adaptation of the Nelson-Somogyi reducing-sugar assay to a microassay using microtiter plates. Anal Biochem 1989;182:197-9.
Anitha A, Rabeeth M. Degradation of fungal cell walls of phytopathogenic fungi by lytic enzyme of Streptomyces griseus. Afr J Plant Sci 2010;4:61-6.
Prapagdee B, Kuekulvong C, Mongkolsuk S. Antifungal potential of extracellular metabolites produced by Streptomyces hygroscopicus against phytopathogenic fungi. Int J Biol Sci 2008;4:330-7.
Quecine MC, Araujo WL, Marcon J Gai, CS Azevedo JL, Pizzirani-Kleiner AA. Chitinolytic activity of endophytic Streptomyces and potential for biocontrol. Lett Appl Microbiol 2008;47:486-91.