ANTIDIABETIC AND ANTIDYSLIPIDEMIC ACTIVITY OF ETHYL ACETATE FRACTIONS OF XYLOCARPUS GRANATUM AND XYLOCARPUS MOLLUCCENSIS ON HIGH FRUCTOSE HIGH FAT AND HIGH SUCROSE HIGH FAT FED-LOW DOSED STREPTOZOTOCIN TREATED DIABETIC RATS

Authors

  • Swayam Prakash Srivastava CSIR-Central Drug Research Institute
  • Akansha Mishra CSIR-Central Drug Research Institute
  • Vijai Lakshmi CSIR-Central Drug Research Institute
  • Akhilesh Kumar Tamrakar CSIR-Central Drug Research Institute
  • Mahendra Nath Srivastava CSIR-Central Drug Research Institute
  • Arvind Kumar Srivastava Senior Principal Scientist & Head, Division of Biochemistry, CSIR-Central Drug Research Institute, Lucknow-226001, India.

Keywords:

HFD fed streptozotocin treated diabetic rats, HSD fed streptozotocin treated diabetic rats, Type 2 diabetes mellitus, Antihyperglycaemic activity, Dyslipidaemic activity, Liver function test, Renal function test

Abstract

Objectives: The present study was carried out to investigate the antidiabetic and antidyslipidemic effect of standardized fractions of X. granatum (CDR-134 F194) and X. molluccensis (CDR-267 F018) by measuring the status of blood glucose, serum insulin, lipid levels, hepatic and renal function markers of high fructose high fed streptozotocin treated rats and high sucrose high fat diet fed-low dosed Streptozotocin treated diabetic rats.

Methods: Male rats of Sprague Dawley strain of body weight around 150 g when kept on high fructose high fat diet and high sucrose high fat diet for two weeks, respectively, showed abnormal glucose tolerance, dyslipidemia and obesity and at this stage when streptozotocin was given intraperitoneally at 45.0 mg/kg body weight caused persistent hyperglycemia in them addition to dyslipidemia along with impairment in their hepatic and renal functions.

Results: The standardized fractions of X. granatum (CDR-134 F194) and X. molluccensis (CDR-267F018) when given to these high fructose high fat fed low dosed streptozotocin treated diabetic rats or high sucrose high fat diet fed-low dosed streptozotocin treated diabetic rats for 10 consecutive days showed significant improvement in their glucose intolerance, decline in their serum triglycerides and LDL-cholesterol levels. These CDR-134 F194 and CDR-267 F018 treated rats also showed elevation in their HDL-cholesterol levels and improvement in their hepatic and renal functions as evidenced by decline in SGOT, SGPT, urea, uric acid and creatinine levels.

Conclusion: The present study thus concludes that the antidiabetic efficacy of standardized fractions of X. granatum (CDR-134 F194) and X. molluccensis (CDR-267F018) have favorable effect in bringing down the severity of hyperglycemia, hyperlipidemia, decline the increased level of renal and hepatic function markers and also improving glucose tolerance activity.

Downloads

Download data is not yet available.

References

Horton ES. Can newer therapies delay the progression of type 2 diabetes mellitus? Endocr Pract 2008;14(5):625-38.

King H, Aubert RE, Herman WH. Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care 1998;21(9):1414-31.

Venkatesh S, Reddy GD, Reddy BM, Ramesh M, Rao AVN. Antihyperglycemic activity of Caralluma attenuata. Fitoterapia 2003;74:274-9.

Suba V, Murugesan T, Arunachalam G, Mandal SC, Saha BP. Anti-diabetic potential of Barleria lupulina extract in rats. Phytomed 2004;11(2-3):202-5.

Lakshmi V, Gupta P. An overview of the genus Xylocarpus. Nat Prod Res 2008;22(14):1197-224.

Zaridah MZ, Idid SZ, Wan Omar A, Khozirah S. In vitro antifilarial effects of three plant species against adult worms of subperiodic Brugia malayi.†J Ethnopharmacol 2001;78(1):79–84.

Uddin SJ, Shilpi JA, Delazar A, Nahar L, Sarker SD. Free radical scavenging activity of some Bangladeshi plant extracts. Oriental Pharm Exp Med 2004;4(3):185-93.

Okorie DA, Taylor DAH. Limonoids from Xylocarpus granatum Koenig. J Chem Soc [Section] C: Organic 1970;2:211-3.

Alvi KA, Crews P, Aalbersberg B, Prasad R. Limonoids from the Fijian medicinal plant Dabi (Xylocarpus). Tetrahedron 1991;47(43):8943-8.

Wu J, Zhang S, Xiao Q, Li Q, Huang J, Xiao Z, et al. Xyloccensin M and N, two new B,D-seco limonoids from Xylocarpus granatum. Z Naturforsch B J Chem Sci 2003;58(12):1216–9.

Wu J, Xiao Q, Huang J, Xiao Z, Qi S, Li Q, et al. Xyloccensins O and P, unique 376 8,9,30-phragmalin ortho esters from Xylocarpus granatum. Org Lett 2004b;6(11):1841-4.

Wu J, Zhang S, Xiao Q, Li Q, Huang J, Long L, et al. Xyloccensin L, a novel limonoid from Xylocarpus granatum. Tetrahedron Lett 2004a;45(3):591-3.

Wu J, Xiao Q, Zhang S, Li X, Xiao Z, Ding H, Li Q. Xyloccensins Q-V, six new 8,9,30-phragmalin ortho ester antifeedants from the Chinese mangrove Xylocarpus granatum. Tetrahedron 2005a;61(35):8382-9.

Wu J, Zhang S, Song Y, Xiao Z, Xiao Q, Li Q. Two new mexicanolides from the fruits of the Chinese mangrove Xylocarpus granatum. Zeitschrift für Naturforschung B Cheml Sci 2005b;60b:1-4.

Wu J, Xiao Z, Song Y, Zhang S, Xiao Q, Ma C, et al. Spectral assignments and reference data: complete assignments of 1H and 13C NMR data for two 3β,8β-epoxymexicanolides from the fruit of a Chinese mangrove Xylocarpus granatum. Magn Reson Chem 2006b;44(1):87-9.

Cui J, Deng Z, Li J, Fu H, Proksch P, Lin W. Phragmalin-type limonoids from the mangrove plant Xylocarpus granatum. Phytochem 2005;66(19):2334-9.

Xiao Z, Wu J, Zhang S, Li Q. Xyloccensin K extracted from Xylocarpus granatum (muguodong) studied by NMR spectroscopy. Bopuxue Zazhi 2005;22(3):315–9.

Chou FY, Hostettmann K, Kubo I, Nakanishi K, Taniguchi M. Isolation of an insect antifeedant Nmethylflindersine and several benz[c]phenanthridine alkaloids from East African plants; a comment on chelerythrine. Heterocycl 1977;7(2):969-77.

Majumdar SG, Patra G. Chemical investigation of some mangrove species. Part II. Carapa obovata Bl. J Indian Chem Soc 1976;53(9):947-8.

Hogg RW, Gillian FT. Fatty acids, sterols and hydrocarbons in the leaves from eleven species of mangrove. Phytochem 1984;23:93-7.

Uddin JS, Nahar L, Shilpi AJ, Shoeb M, Borkowski T, Gibbons S, et al. Gedunin, a Limonoid from Xylocarpus granatum, Inhibits the Growth of CaCo-2 Colon Cancer Cell Line In Vitro. Phytother Res 2007;21:757-61.

Heather Basciano, Lisa Federico, Khosrow Adeli. Fructose, insulin resistance, and metabolic dyslipidemia. Nutr Metab 2005;2:5.

Aurélie Girard MS, Sihem Madani, Farida Boukortt, Mustapha Cherkaoui-Malki, Jacques Belleville, Josiane Prost. Fructose-enriched diet modifies antioxidant status and lipid metabolism in spontaneously hypertensive rats. Nutr 2006;22:758-66.

Wan Huang, Nikolas Dedousis, Robert M, O’Doherty. Hepatic steatosis and plasma dyslipidemia induced by a high-sucrose diet are corrected by an acute leptin infusion. J Appl Physiol 2007;102:2260-65.

Ingrid J Hickman, Graeme A Macdonald. Impact of diabetes on the severity of liver disease. Am J Med 2007;120:829-34.

MJ Reed, K Meszaros, LJ Entes, MD Claypool, JG Pinkett, TM Gadbois, et al. A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat. Metab 2000;49(11):1390-4.

K Srinivasan, B Viswanad, Lydia Asrat, CL Kaul, P Ramarao. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res 2005;52(4):313-20.

Mishra A, Srivsatava R, Srivastava SP, Gautam S, Tamrakar AK, Maurya R, et al. Antidiabetic activity of heart wood of Pterocarpus marsupium Roxb and analysis of phytoconstituents. Indian J Exp Biol 2013;51(5):363-74.

P Bwititi, CT Musabayane, CFB Nhachi. Effects of Opuntia megacan-tha on blood glucose and kidney function in streptozotocin diabetic rats. J Ethnopharmacol 2000;69(3):247–52.

Srivsatava R, Srivastava SP, Jaiswal N, Mishra A, Maurya R, Srivastava AK. Antidiabetic and antidyslipidemic activities of Cuminum cyminum L. in validated animal models. Med Chem Res 2010;20(9):1656-66.

Mishra A, Srivsatava R, Srivastava AK. Comparative antidiabetic profile of ayurvedic herbo-mineral formulation and its constituents on normal and streptozotocin-induced diabetic rats. Int J Pharm Sci Rev Res 2013;22(2):252-63.

Duseja A, Das A, Dhiman RK, Chawla YK, Thumburu KT, Bhadada S, et al. Metformin is effective in achieving biochemical response in patients with nonalcoholic fatty liver disease (NAFLD) not responding to lifestyle interventions. Ann Hepatol 2007;6(4):222-6.

Chavez-Tapia NC, Barrienos-Gutierrez T, Tellez-Avila Felix I, Sanchez-Avila F, Montanes-Reyes MA, Uribe M. Insulin sensitizers in treatment of nonalcoholic fatty liver disease: systematic review. World J Gasteroenterol 2006;12(48):7826-31.

Published

01-02-2015

How to Cite

Srivastava, S. P., A. Mishra, V. Lakshmi, A. K. Tamrakar, M. N. Srivastava, and A. K. Srivastava. “ANTIDIABETIC AND ANTIDYSLIPIDEMIC ACTIVITY OF ETHYL ACETATE FRACTIONS OF XYLOCARPUS GRANATUM AND XYLOCARPUS MOLLUCCENSIS ON HIGH FRUCTOSE HIGH FAT AND HIGH SUCROSE HIGH FAT FED-LOW DOSED STREPTOZOTOCIN TREATED DIABETIC RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 2, Feb. 2015, pp. 537-43, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/4970.

Issue

Section

Original Article(s)

Most read articles by the same author(s)