DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF VOGLIBOSE IN PHARMACEUTICAL DOSAGE FORMS

Authors

  • Charushila C. Shinde Bharati Vidyapeeth University
  • Shakuntala Chopade Bharati Vidyapeeth University
  • Suneela S. Dhaneshwar Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University , Poona College of Pharmacy , Pune , Maharashtra , India-411038. suneeladhaneshwar@rediffmail.com

Keywords:

Voglibose, HPTLC, Densitometric estimation, Method development, Validation

Abstract

Objective: This paper describes a new, simple, precise, accurate and specific HPTLC method for estimation of voglibose as a bulk drug and in tablet dosage forms.

Methods: Chromatographic separation of the drug was performed on aluminum plates pre-coated with silica gel 60 F254 as the stationary phase and a mobile phase comprising of toluene: ethyl acetate: methanol: 30% ammonia 5:4:1.5:0.2 (v/v/v/v). Densitometric quantification of voglibose was carried out at 292 nm. Voglibose was detected satisfactorily with a Rf value 0.26.

Results: The accuracy and reliability of the method was assessed by evaluation of linearity (0.2-1.2 µg/spot), precision (intra-day RSD 0.6-0.9% and inter-day RSD 0.20-0.25%), accuracy (97.32-102.46%) and specificity according to ICH guidelines.

Conclusion: The proposed HPTLC method is less expensive, simpler, rapid and more flexible than the reported RP-HPLC method for routine analysis of voglibose in bulk and tablet dosage forms.

 

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Author Biography

Suneela S. Dhaneshwar, Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University , Poona College of Pharmacy , Pune , Maharashtra , India-411038. suneeladhaneshwar@rediffmail.com

CURRICULUM VITAE of Scientist mentor:

 

1. Name: Dr. (Mrs.) Suneela Sunil Dhaneshwar

  M. Pharm. Ph. D. (Pharmaceutical Chemistry) Devi Ahilya Vishwavidyalaya,  Indore.

2.         Designation:               Professor of Pharmaceutical Chemistry.

3. Institution/Organization:   Department of Pharmaceutical Chemistry, Poona College of                                                                        Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane, Pune, 411 031.

4. Teaching experience: 20 yrs; Research experience: 23 yrs;

 Industrial experience: 2 yrs, Consultancy: 4 years ( LG Life Sciences, South Korea; SACOOR MEDICAL GROUP;  International Pharmaceutical Industry Consultants, West Byfleet, Surrey, UK.)

  Approved PG. Teacher of Bharati Vidyapeeth University and University of Pune.

   Approved Ph.D. Guide of Bharati Vidyapeeth University and University of Pune.

  1. Patents: 01[Mutual Prodrugs for Colon Specific Drug Delivery: Suneela S Dhaneshwar, Gaurav Vadnerkar and S.S. Kadam. Application No. 248/MUM/2008]                

  2. Publications (52), Total Impact Factor: 65.879, Citation Index: 250

  3. Research Papers Presented in the International Conference: 18

  4. Research Papers Presented in the National Conference: 37

  5. Honours and awards: 22

  • Best Article Award of Indian Journal of Pharmaceutical Education (IJPE) at APTI International Convention at VIZAG.

  • International Best Poster Award from Academy of Pharmaceutical Sciences of Great Britain at British Pharmaceutical Conference – 2006 organized by Royal Pharmaceutical Society of Great Britain .

  • Prof. S. B. Sonawane Memorial Best Paper award in Pharmaceutical Chemistry for the year 2006 for research paper published in Indian Journal of Pharmaceutical Education and Research.

  • 54th IPC Trust’s Research Project of the Year Award 2007 for the projectat the hands of Mr. Harshawardhan Patil, Cabinet Minister, Government of Maharashtra.

  • Prof. M. L. Khorana Memorial Best Paper award in Pharmaceutical and Medicinal Chemistry for the year 2006 for research paper.

  • Best Paper Award (I Prize) for research paper,  was awarded during the 14th Foundation Day of Bharati Vidyapeeth University on 26th April, 2009 at the hands of Vice-chancellor Dr. Shivajirao Kadam and in presence of eminent poet Mr Mangesh Padgaonkar and Mr. Anand Yadav.  

  • Ranbaxy Research Foundation’s Award 2009, for the Project related with IBD.

  • The BEST PRESENTATION AWARD for PHARMAGLOW’S NATIONAL PHARMA PPT COMPETITION – 2009, organized by PHARMAIN.NET.

  • Best oral presentation at University of Pune organized international conference on Emerging trends in chemistry for the work DESIGN AND DEVELOPMENT OF GASTROSPARING PRODRUG OF ACECLOFENAC,.

  • American Association of Indian Pharmaceutical Scientists sponsored AAiPS Distinguished Educator and Researcher Award of 4000$ grant for the year 2010, at the New Orleans Ernest N. Morial Convention Center in New Orleans, LA.

  • Best Oral Presentation at International Conference on Recent Trends in Drug Discovery at University Department of Pharm Sci.. Kakatiya University, Warangal ,SEPT 2010.

  • Best Presentation Award to Samuel Soares, Gaurav Vadnerkar & Suneela Dhaneshwar for the paper Betacyclodextrin-4-aminosalicylic acid prodrug for colon-targeted delivery for inflammatory bowel disease managment†presented in Young Researcher’s Conference organized by Institute of Chemical Technology (UDCT), Mumbai on 10th -13th Jan 2013.

  • Best Poster Award to Dipmala Patil & Suneela Dhaneshwar for the paper Design & development of diacerein codrug for safe management of osteoarthritis†at the Young Researcher’s Conference organized by Institute of Chemical Technology (UDCT), Mumbai on 10th -13th Jan 2013.

  • Invited as a Guest speaker at Prof. Tamara Minko, Chair Dept. of Pharmaceutics and Prof. Patrick Sinko’s Labs at Rutger’s University, NJ, USA and Bristol-Meyers-Squibb NJ, USA.

  • Invited as a Guest speaker at International Conference on Recent Trends in Drug Discovery at University Department of Pharm Sci. Kakatiya University, Warangal   SEPT 2010.

  • Invited as a Guest speaker at International Conference OMICS Pharmaceutica-2012, 2nd World Congress on Pharmaceutics &Novel Drug Delivery Systems, 20-22 February 2012 San Francisco, USA.

  • II Prize poster presentation  at AICTE SPONSORED  2 days National Conference on Advanced Bioanalytical  Techniques (NCABT- 2013) organized by  Poona College of Pharmacy, Pune, 21-22nd Sep 2013.

  • III Prize oral presentation, at AICTE SPONSORED  2 days NATIONAL CONFERENCE ON ADVANCES IN BIOANALYTICAL TECHNIQUES (NCABT- 2013) organized by  Poona College of Pharmacy, Pune, 21st  and 22nd Sept.

  •  P. D. Sethi Best paper award for the paper Synthesis and pharmacokinetic profile of rhein- boswellic acid conjugate, Suneela Dhaneshwar and  Dipmala Patil, Bioorganic and Medicinal Chemistry Letters, 22(24), 2012, 7582-7587  

  • Green synthesis of thioester prodrug of N –acetyl cysteine, Suneela Dhaneshwar, Neha Bhilare,  Kashmira Patil, International conference on  Solid Waste Management Technology, Poona College of Pharmacy, Pune, 8-9th Jan 2014 (I Prize Poster Presentation).

  • Neha Bhilare , Kashmira Patil, Poorvashree Joshi and Suneela Dhaneshwar, Green synthesis of thioester prodrug: a comparative study between conventional and microwave- assisted synthesis, AICTE sponsored Innovation Promotion Scheme (IPS)-2013-14 Two Days National Symposium on Young Minds In Pharmaceutical Innovation & Technical Events.(II Prize Poster Presentation)

  • Mrunal Bulbule, Vedang Kinjawadekar, Dhaval Joshi, Vineet Joshi, Kasmira Patil, Suneela Dhaneshwar, Photosensitizers and photodynamic therapy for cancer, AICTE sponsored Innovation Promotion Scheme (IPS)-2013-14 Two Days National Symposium on Young Minds In Pharmaceutical Innovation & Technical Events. (II Prize Poster Presentation)

 

  1. Achievements:

  • Member- Editorial Board of World Journal of Gastroenterology, China.

  • Member- Editorial Board of Pharmacologia, UK.

  • Member- Editorial Board of Indian Journal of Pharmaceutical Sciences, Kalina, Mumbai.

  • Member- Editorial Board of International Journal of Pharmaceutical, Chemical and Biological Sciences, India.(Jan 2012)

  • Member- Editorial Board of Journal of Pharmaceutical Research and Technology, Bhopal.

  • Lead Discovery’s Daily update service (UK) has chosen the Research Paper Colon-specific mutual amide prodrugs of 4-aminosalicylic acid for their mitigating effect on experimental colitis in rats†published in European J. of Medicinal Chemistry for listing in Immunology & Inflammatory Diseases channel of Daily Updates. This service circulates BREAK THROUGH RESEARCH ARTICLES to the scientific community all over the world.

  • Invited as a Reviewer for Pre-publication E-book Proposal for Bentham Science Publishers.

  • Reviewer for Indian Council of Medical Research (ICMR) Fellowships for Full-time Research Scholars.

  • Felicitation as WOMAN ACHIEVER on the occasion of International Women’s Day at IMED , Bharati Vidyapeeth University , 8th March, 2009.

  • Approved Inspector of Pharmacy Council of India.

  • Resource Person on Prodrug Design and Applications

  • Total number of invited lectures (as resource person/guest speaker ):50

  • Member, Board studies in Pharmacy, Bharati Vidyapeeth University (2009-2011).

  • Invited member, Board studies in Pharmaceutical Chemistry, University of Pune.

  • Member, Local Management Committee, Bharati Vidyapeeth University.

  • Coordinator for Quality Improvement Progrramme for Pharmacy Teachers on Emerging Frontiers in Drug Discovery from 1st-13th Feb 2010.

  • Co-chairman Scientific Committee Polytech 2012: An international conference organized by BVDU, NCL, CMAT, and SPS India.

  • Co-chairman Scientific Committee and Co-chairman Poster session of AICTE-sponsored national conference on advances in bioanalytical techniques organized by PCP, Pune during 21-22nd Sep 2013.

  • Co-chairman Scientific Committee and Co-chairman Oral session of International Conference SWMT 2014 on 8-9th Jan 2014 at Poona College of Pharmacy, Pune.

  • Member BOS, LMC, BVDU, Pune

  1. Reviewer for 26 International Journals

  2. Completed Funded Projects worth 90 lakh from AICTE, UGC, ICMR.

  3. No. of Ph .D. Students Guided: Awarded:01, Pursuing:04

  4. No. of M. Pharm. Students Guided: 30

     

     

  5. Current Research Areas of Interest:

  • Design and development of prodrugs,Colon specific drug delivery, Analytical & bioanalytical method development

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Published

01-05-2015

How to Cite

Shinde, C. C., S. Chopade, and S. S. Dhaneshwar. “DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF VOGLIBOSE IN PHARMACEUTICAL DOSAGE FORMS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 5, May 2015, pp. 288-93, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/5101.

Issue

Vol 7, Issue 5, 2015

Section

Original Article(s)