ANTIMICROBIAL SUSCEPTIBILITY AND PREVALENCE OF EXTENDED SPECTRUM BETALACTAMASE (ESBL) AND METALLO BETALACTAMASE (MBL) AND ITS CO-EXISTENCE AMONG PSEUDOMONAS AERUGINOSA RECOVERED FROM OCULAR INFECTIONS

Authors

  • Nandagopal Murugan Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India-600 006. Ph.D., Scholar, School of Chemical & Biotechnology, SASTRA University, Thanjavur, Tamil Nadu, India- 613401.
  • Jambulingam Malathi Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India-600 006.
  • Kulandai Lily Therese Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India-600 006.
  • Hajib Naraharirao Madhavan Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India-600 006.

Keywords:

Antimicrobial resistance, Pseudomonas aeruginosa, Keratitis, Ocular infections

Abstract

Objectives: To evaluate the changing trends in antimicrobial susceptibility rate and detection of ESBL and MBL among Pseudomonas aeruginosa isolated from various ocular infections over a 2 year periods with special reference to detection of ESBL and MBL co-existence among P aeruginosa recovered from ocular infections.

Methods: All ocular specimens, culture positive for P aeruginosa (n=110) isolated from clinically suspected patients were submitted to L &T Microbiology Research Centre, Chennai, Tamil Nadu, India. Culture, antimicrobial susceptibility testing and ESBL detection was performed by Standard methods. MBL production was screened by Carbapenem-EDTA combination disk method.

Results: Of the 3247 samples subjected to culture from August 2012– July 2014 by standard method 276 were positive for bacterial growth, thereby 8.5% of ocular infections mediated by bacterial pathogens. Out of 276 culture positives 110 (39.8%) Pseudomonas aeruginosa isolates recovered from ocular infections. The resistance rate for commonly used drugs against ocular infection includes Gentamycin [23.63%], Gatifloxacin [20.9%], Moxifloxacin [20%], Tobramycin [20%], ciprofloxacin [19.09%]. Totally 15 (13.63%) out of 110 isolates were identified as ESBL producer and 11 (10%) out of 110 isolates were identified as MBL producer by screening test, including 7 isolates have co-produced both ESBL and MBL enzymes and 4 isolates were only positive for MBL production.

Conclusion: Though fluoroquinolones remains a good choice for ocular Pseudomonal infection. Gradual emergence of resistance to fluoroquinolones and aminoglycosides also noted from this study. The emergence of ESBL, MBL and pandrug resistance among P aeruginosa from ocular infections is an alarm rational finding which necessitates the earlier detection of both ESBL and MBL production as individual or co-existence in ocular isolates, which may pave the way for appropriate therapy for sight threatening conditions like endophthalmitis.

 

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Author Biography

Jambulingam Malathi, Dept of Microbiology, L & T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, Tamil Nadu, India-600 006.

L & T Microbiology Research Centre

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Published

01-05-2015

How to Cite

Murugan, N., J. Malathi, K. L. Therese, and H. N. Madhavan. “ANTIMICROBIAL SUSCEPTIBILITY AND PREVALENCE OF EXTENDED SPECTRUM BETALACTAMASE (ESBL) AND METALLO BETALACTAMASE (MBL) AND ITS CO-EXISTENCE AMONG PSEUDOMONAS AERUGINOSA RECOVERED FROM OCULAR INFECTIONS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 5, May 2015, pp. 147-51, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/5274.

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