ANTI-OXIDANT AND ANTI-MICROBIAL ACTIVITIES OF SYNTHETIC 3-FORMYL, 7-FLAVONOL INTERMEDIATES OBTAINED BY MICROWAVE ASSISTED TECHNIQUE
Keywords:
3-formyl, 7-flavonol, Anti-microbial, Disc diffusion method, Anti-oxidant, Hydrogen peroxide method, Nitric oxide method, Alkaline DMSO methodAbstract
Objective: The synthesized compounds of 3-formyl, 7-flavonols*, after characterization, aimed to be tested for their anti-oxidant and anti-microbial activities.
Methods: i) anti-oxidant activities by hydrogen peroxide-nitric oxide-and by alkaline DMSO-methods and ii) anti-microbial activities against various gram-negative and gram-positive pathogens and against candida albicans by disc diffusion method.
Results: Findings were found to be dose dependent and IC50 value was 30-60 µg/ml and the results revealed that the dinitro-, trinitro-and acetyl, dinitro derivatives showed better and/or equipotent activity to that of the standard, ascorbic acid. The synthesized compounds at a concentration of (1 μg/10 μl/disc) showed variable inhibitory activities against all bacteria with inhibition zone diameters ranging from 7-26 mm and a good antifungal activity against Candida albicans at the concentration of (1 μg/10 μl/disc) with inhibition of 10-24 mm. Klebsiella tribatta are more susceptible to the action of the formylated samples, giving high inhibition values comparing to the other organisms. Compounds Ie and Ih resulted to a higher activity index (AI>1); compounds Id, Ig and Ii showed an equal value (AI=1); whereas, Ia, Ib, Ic and If showed only a moderate activity (AI<1) compared to the standard, Amikacin.
Conclusion: The findings confirmed that the synthetic compounds of 3-formyl, 7-flavonol derives have significant anti-oxidant and anti-microbial activities.
*Synthesis and characterization work of 3-formyl, 7-flavonols has already been accepted for publication by the journal Elseveir, Procedio Chemistry and is in process.
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