PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND HYPOGLYCEMIC ACTIVITY OF COCCOLOBA UVIFERA LEAVES AND WALTHERIA INDICA ROOTS EXTRACTS

Authors

  • Lawson-evi Povi Laboratory of Physiology/Pharmacology University of Lomé BP 1515 Togo
  • Bakoma Batomayena University of Lomé
  • TITRIKOU AFI HODE University of Lomé
  • EKLU-GADEGBEKU KWASHIE University of Lomé
  • Aklikokou Kodjo University of Lomé
  • Gbeassor Messanvi University of Lomé

Keywords:

hypoglycemia, Oral glucose tolerance test, Antioxidant, Coccoloba uvifera, Waltheria indica

Abstract

Objective: Coccoloba uvifera (Polygonaceae) and Watheria indica (Sterculiaceae) are two medicinal plants used in Togolese traditional medicine to treat diabetes mellitus. The present study was designed to evaluate their antioxidant properties and hypoglycemic activity.

Methods: After phytochemical screening, hydro alcoholic leaves extracts of Coccoloba uvifera and the hydro alcoholic roots extract of Watheria indica were evaluated on blood glucose levels in fasting normal and glucose loaded hyper glycemic rats (oral glucose tolerance test). Antioxydant activities were performed using AAPH (2, 2'-Azobis 2 Amidino propane Dihydrochloride test and nitric oxide radical scavenging activity).

Results: Phytochemical tests revealed the presence of flavonoids, total phenols and tanin in the hydro alcoholic extracts of C. uvifera and W. indica. The hypoglycemic activity measured after oral administration of glucose (2 g/kg) revealed that W. indica leaves and C. uvifera roots induced a significant reduction of hyperglycemia in treated group compared to control group. There was a significant reduction in the hyperglycemic peak with W. indica leaves extract at doses of 250 mg/kg (p<0.05) and 500 mg/kg (p<0.001). Moreover, the administration of extracts (at dose of 500 mg/kg) in fasted rats did not show any significant decrease in basal blood glucose level compared to the control. Hydro alcoholic extracts inhibit hemolysis of erythrocytes induced by AAPH dose-dependent manner and have an antioxidant power comparable to that of the Butylated Hydroxy Toluene (reference drug). The extracts inhibit the production of nitric oxide and possess reducing power.

Conclusion: The results of this study support the use of these plants in the treatment of diabetes in Togolese traditional medicine.

 

Downloads

Download data is not yet available.

Author Biography

Lawson-evi Povi, Laboratory of Physiology/Pharmacology University of Lomé BP 1515 Togo

Lome

References

Bhat M, Smita S, Zinjarde SS, Bhargava Y, Ameeta Ravi Kumar AR, Joshi BN. Antidiabetic Indian Plants: A Good Source of Potent Amylase Inhibitors. 2008. p. 6.

Wild S, Roglic G, Green A, Sicree R. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.

Fédération internationale du diabète (FID), Professeur Jean Claude Mbanya, Plan mondiale contre le diabète; 2011. p. 4.

Agoudavi k. Ministry of Health of Togo. Final Report of the STEPS Togo 2010 investigation; Ministry of Health Togo; 2012.

Rates SMK. Review: Plants as source of drugs. Toxicon 2001;39:603-13.

Vitor R, Mota-Filipe H, Teixeira G, Borges C, Rodrigues A, Teixeira A, et al. Flavonoids of an extract of Pterospartum tridentatum showing endothelial protection against oxidative injury. J Ethnopharmacol 2004;93(2–3):363-70.

Ghedira K. Les flavonoïdes: structure, propriétés biologiques, rôle prophylactique et emplois en thérapeutique. Phytother 2005;3(4):162-9.

Chen HQ, Jin ZY, Wang XJ, Xu XM, Deng L, Zhao JW. Luteolin protects Dopaminergic neurons from inflammation-induced injury through inhibition of microglial activation. Neurosci Lett 2008;448(2):175-9.

Marfak A. Gamma radiolysis flavonoids: study of their reactivity with radicals derived alcohols; training depsides: Ph D thesis, University of Limoges; 2003. p. 24-42.

Kadebe Zoua, Bakoma B, Metowogo K, Lawson-Evi P, Eklu-Gadegbeku K, Aklikokou Kodjo, et al. Effects of Plumeria alba roots hydro alcoholic extract on some parameters of type 2 diabetes. Res J Med Plant 2014;8:140-8.

Lawson–Evi P, Eklu-Gadegbku K, Agbononon A, Aklikokou K, Creppy EE, et al. Antidiabetis activity of Phyllanthus amarus on alloxan induceddiabetes in male wistar rats. J Appl Sci 2011;1:2968-73.

Wiernsperger N. Oxidative stress as a therapeutic target in diabetes: revisiting the controversy. Diabetes Metab 2003;29:579-85.

Robertson R. Chronic oxidative stress as a central mechanism for glucose toxicity in pancreatic islet beta cells in diabetes. J Biol Chem 2004;279:42351-4.

Harbone JB. Phytochemical methods. Chapman and hall. Eds. News York; 1973. p. 354.

Maksimovic Z, Malencic D, Kovacevic N. Polyphenol contents and antioxidant activity of Maydis stigma extracts. Bioresour Technol 2005;96:873-77.

Mimica-Dukic N. Investigation on secondary biomolecules in some Mentha-species. Thesis. University of Novi Sad; 1992. p. 150.

Bakoma B, Eklu-Gadegbeku K, Berké B, Diallo A, Agbonon A, Aklikokou K, et al. Total phenolic content, antioxidant activity and in vitro inhibitorypotential against key enzymes relevant for hyperglycemia of Bridelia ferruginea extracts. Res J Phytochem 2012;6:120-6.

Oyaizu M. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Japen J Nutr 1986;44:307-15.

Sreejayan, Rao MN. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997;49(1):105-8.

Fontaine P. Physiologie de la glycorégulation. Problème Quotidien du Diabète 1987;163:10-1.

Saenz A, Fernandezâ€Esteban I, Mataix A, Ausejo Segura M, Roqué-Figuls M, Moher D. Metformin monotherapy for type 2 diabetes mellitus. Cochrane Database of Systematic Reviews; 2005. p. 3.

Marfak A. Radiolyse gamma flavonoids: study of their reactivity with radicals derived alcohols; despised training. University Doctoral Thesis Limoges 2003. p. 24-42.

Ceriello A. Postprandial hyperglycemia and diabetes complications. Diabetes 2005;54:1-7.

Martin-Nizard F, Saphpaz S, Kandoussi A, Carpentier M, Fruchart J, Duriez P. Natural phenylpropanoidsinhib lipoprotein induced endothelim-1 secretion by endothelials cells. J Pharm Pharmacol 2004;56:1607-11.

Heim EK, Tagliaferro RA, Bobilya JD. Flavonoids antioxidant: chemistry metabolism and structure activity relationship. J Nutr Biochem 2002;13:572-84.

Fidrianny IP, Komar U, Ruslan W. Evaluation of antioxidant capacities, flavonoids, phenolic, carotenoids content from various extracts of four kinds brassica herbs. Int J Pharm Pharm Sci 2014;6:268-72.

Published

01-05-2015

How to Cite

Povi, L.- evi, B. Batomayena, T. A. HODE, E.-G. KWASHIE, A. Kodjo, and G. Messanvi. “PHYTOCHEMICAL SCREENING, ANTIOXIDANT AND HYPOGLYCEMIC ACTIVITY OF COCCOLOBA UVIFERA LEAVES AND WALTHERIA INDICA ROOTS EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 5, May 2015, pp. 279-83, https://mail.innovareacademics.in/journals/index.php/ijpps/article/view/5523.

Issue

Section

Original Article(s)

Most read articles by the same author(s)