METABOLITES ACTIVITY OF ENDOPHYTIC STREPTOMYCES SP. IPBCC. B.15.1539 FROM TINOSPORA CRISPA L. MIERS: ALPHA-GLUCOSIDASE INHIBITOR AND ANTI-HYPERGLYCEMIC IN MICE
Keywords:
Streptomyces sp, Tinospora crispa, Nil, Anti-hyperglycemic, miceAbstract
Objective: This research work aimed to assess the capability of endophytic Streptomyces sp. IPBCC. b.15.1539 isolated from Tinospora crispa inproducing α-glucosidase inhibitor compound and examined the effect of its ethyl acetate extract containing α glucosidase inhibitor in lowering blood glucose in streptozotozin mice.
Methods: Streptomyces sp. IPBCC. b.15.1539 was grown in a bioreactor filled with International Streptomyces Project 2 medium, for 5, 10, 15, and
20 days, and assayed for its in vitro α-glucosidase inhibitory activity. The ethyl acetate extract was examined for its IC50
Results: The crude extract produced value. An in vivo experiment
was set up using thirty mice, which were divided into five treatment groups: (a) acarbose (0.03 mg/30 g body weight) used as a positive control, (b) placebo used as a negative control, (c-e) treatment groups were treated with ethyl acetate extract at 0.036 mg/30 g body weight (P1), 0.36 mg/30 gbody weight (P2), 0.036 mg/30 g body weight (P3).
98.5% α-glucosidase inhibitory activity, with 15,6 biomass after 10 days of production. The ethyl acetateextract at a concentration of 1000 µg/ml produced 96.08% α-glucosidase inhibitory activity, while acarbose at the same concentration gave 97.46%
inhibition. The IC50 for the ethyl acetate extract
Conclusions: The was 0.047 µg/ml, while for acarbose was 0.003 µg/ml. The ethyl acetate extract applied as the P1
treatment group lowered blood glucose levels in streptozotozin mice by 26%. α-glucosidase inhibitor of Streptomyces sp. IPBCC. b.15.1539 of T. crispa has the potency as an antidiabetic agent for type 2 DM
therapy.
Downloads
References
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.
[WHO] World Health Organization. 10 Facts about Diabetes. Geneva; 2014.
Laube H. Acarbose. An update of its therapeutic use in diab etes treatment. Clin Drug Invest 2002;22:141-56.
Wehmeier UF, Piepersberg W. Biotechnology and molecular biology of theï€ ï¡-glucosidase inhibitor acarbose. Appl Microbiol Biotechnol 2004;63:613-25.
Bnouham M, Ziyyat A, Mekhfi H, Tahri A. Medicinal plants with potential antidiabetic activity–a review of ten years of herbal medicine research. Int J Diabetes Metab 2006;14:1-25.
Klein G, Jaekyung Kim, Klaus Himmeldirk, Yanyan Cao, Xiaozhuo Chen. Antidiabetes and anti-obesity activity of Lagerstroemia speciosa. eCAM 2007;4:401-7.
Lamba HS, Bhargava CS, Thakur M, Bhargava S. Alpha glucosidase and aldolase reductase inhibitory activity in vitro and anti diabetic activity in vivo of Tribulus terrestris L. (Dunal). Int J Pharm Pharm Sci 2011;3(3):270-2.
Morshed MA, Haque A, Rokeya B, Ali L. Anti-hyperglycemic and lipid lowering effect of Terminalia arjuna bark extract on streptozotozin induced type 2 diabetic model rats. Int J Pharm Pharm Sci 2011;3(4):449-53.
Patela MB, Mishrab SM. Magnoflorine from Tinospora cordifolia stem inhibits α-glucosidase and is antiglycemic in rats. J Funct Foods 2012;4:78-86.
Puranik N, Kammar KF, Devi S. Anti-diabetic activity of Tinospora cordifolia (Willd) in Streptozotozin diabetic rats; does it act like surfonylureas? Turk J Med Sci 2010;40:265-70.
Subramanian R, Asmawi MZ, Sadikun A. Effect of ethanolic extract of Andrographis paniculata nees on a combination of fat-fed diet and low dose streptozotozin induced chronic insulin resistance in rats. Diabetol Croat 2008;37:13-22.
Upwar N, Patel R, Waseem N, Mahobia NK. Hypoglycemic effect of methanolic extract of Berberis aristata DC stem on normal and streptozotozin induced diabetic rats. Int J Pharm Pharm Sci 2011;3(1):222-4.
Noor H, Aschroft SJH. Pharmacological characterisation of the antihyperglycemic properties of Tinospora crispa extract. J Ethnopharm 1998;62:7-13.
Sriyapai C, Rawadee DU, Somkiat S, Ngampong, Sarinee K. Hypoglycemic effect of Tinospora crispa dry powder in outpatients with metabolic syndrome at king chulalongkorn memorial hospital. J Health Res 2009;23:125-33.
Grover JK, Vats V, Rathi SS, Dawar R. Tradition Indian antidiabetic plants attenuate progressive renal damage in streptozotozin induced diabetic mice. J Ethnopharmocol 2003;81:233-40.
Stratmann A, Mahmud T, Lee S, Distler J, Floss HG, Piepersberg W. The AcbC Protein from Actinoplanes Species Is a C7-cyclitol synthase related to 3-dehydroquinate synthases and is involved in the biosynthesis of the α-glucosidase inhibitor acarbose. J Biol Chem 199;274:10889-96.
Zhang. Identification of a 1-epi-valienol 7-kinase activity in the producer of acarbose, Actinoplanes sp. FEBS Lett 2003;540:53-7.
Choi BT, Shin CS. Reduced formation of byproduct component c in acarbose fermentation by Actinoplanes sp. CKD485-16. Biotechnol 2003;19(6):1677-82.
Rockser Y, Wehmeier UF. The gac-gene cluster for the production of acarbose from Streptomyces glaucescens GLA. O: Identification, isolation and characterization. J Biotechnol 2008;140:114-23.
Suthindhiran K. Alpha glucosidase and alpha amylase inhibitory activity of Micromonospora sp. VITSDK3 (EU551238). IJIB 2009;6(3):115-20.
Wei S. Medium optimization for acarbose production by Actinoplanes sp. A56 using the response surface methodology. Afr J Biotechnol 2010;9(13):1949-54.
McGown J. Diabetes drug produced by a microbe in out of africa: mysteries of access and benefit sharing. Beth Burrows (Ed). Washington (US): The Edmonds Institute; 2006.
Pujiyanto S, Yulin L, Antonius S, Sri B, Latifah KD. Alpha-glucosidase inhibitor activity and characterization of endophytic actinomycetes isolated from some Indonesian diabetic medical plants. Int J Pharm Pharm Sci 2012;4:328-33.
Moon HE, Islam MN, Ahn Br, Chowdry SS, Shin HS, Jung HA, et al. Protein tyrosine 1 B and α-glucosidases inhibitory phylotonins from edible brown algae, Eicklonia stolonifera and Eisena bicyclis. Biosci Biotechnol Biochem 2011;75:1472-80.
Pujiyanto S. The activity of α-glucosidase inhibitor and characterization of endophytic actinomycetes isolated from several antidiabetes medicinal plants. [Disertation]. Bogor Agricultural University; 2012.
Kambouche N, Merah B, Derdour A, Bellahouel S, Bouayed J, Dicko A, Younos C. Hypoglycemic and antihyperglycemic effects of Anabasis articulata (Forssk) Moq (Chenopodiaceae), an Algerian medicinal plant. Afr J Biotechnol 2009;8:5589-94.
Wu KK, Youming H. Streptozotozin-induced diabetic models in mice and rats. Pharmacol 2008;47:1-14.
Maier RM, Pepper IL, Gerba CP. Environmental Microbiology. Canada (CA): Academic Pr; 2000.
Waites. Industrial Microbiology: An Introduction. London (UK): Blackwell Science; 2001.
Mahmud T. The C7N aminocyclitol family of natural products. Nat Prod Rep 2003;20:137-66.
Ghadyale V, Shrihari T, Vivek H, Akalpita A. Effective control of postprandial glucose level through inhibition of intestinal alpha glucosidase by Cymbopogon martinii (Roxb.). eCAM 2012;10:1-6.
Jun. Comparisont of antioxidant activities of isoflavones from kudzu root (Pueraria labata ohwl). J Food Sci 2003;68:2117-22.
Tadera K, Yuji M, Kouta T, Tomoko M. Inhibition of α-glucosidase and α-amylase by flavonoids. J Nutr Sci Vitaminol 2006;52:149-53.
Bramachari G. Bio-flavonoids with promising antidiabetic potentials: A critical survey. Res Signpost India 2011;1:187-212.
Fawzy C, Hossam MA, Mohammed SAM, Fathy MS, Amani AS. Antidiabetic and antioxidant activities of major flavonoids of Cynanchum acutum L. (Asclepiadaceae) Growing in Egypt. Z Naturforsch 2008;63:658-62.
Mahesh T, Menon PV. Quercetin alleviates oxidative stress in streptozotozin induced diabetic rats. Phytother Res 2004;18:123-7.